Investigation of bone formation using calcium phosphate glass cement in beagle dogs
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Purpose: Among available biomaterials, bioceramics have drawn special interest due to their bioactivity and the possibility of tailoring their composition. The degradation rate and formulation of bioceramics can be altered to mimic the compositions of the mineral phase of bone. The aim of this study was to investigate the bone formation effect of amorphous calcium phosphate glass cement (CPGC) synthesized by a melting and quenching process. Methods: In five male beagle dogs, 4 × 4 mm 1-wall intrabony defects were created bilaterally at the mesial or distal aspect of the mandibular second and fourth premolars. Each of the four defects was divided according to graft materials: CPGC with collagen membrane (CM), biphasic calcium phosphate (BCP) with CM, CM alone, or a surgical flap operation only. The dogs were sacrificed 8 weeks post-surgery, and block sections of the defects were collected for histologic and histometric analysis. Results: There were significant differences in bone formation and cementum regeneration between the experimental and control groups. In particular, the CPGC and BCP groups showed greater bone formation than the CM and control groups. Conclusions: In conclusion, CPGC was replaced rapidly with an abundant volume of new bone; CPGC also contributed slightly to regeneration of the periodontal apparatus.
Abstract
Purpose: Among available biomaterials, bioceramics have drawn special interest due to their bioactivity and the possibility of tailoring their composition. The degradation rate and formulation of bioceramics can be altered to mimic the compositions of the mineral phase of bone. The aim of this study was to investigate the bone formation effect of amorphous calcium phosphate glass cement (CPGC) synthesized by a melting and quenching process. Methods: In five male beagle dogs, 4 × 4 mm 1-wall intrabony defects were created bilaterally at the mesial or distal aspect of the mandibular second and fourth premolars. Each of the four defects was divided according to graft materials: CPGC with collagen membrane (CM), biphasic calcium phosphate (BCP) with CM, CM alone, or a surgical flap operation only. The dogs were sacrificed 8 weeks post-surgery, and block sections of the defects were collected for histologic and histometric analysis. Results: There were significant differences in bone formation and cementum regeneration between the experimental and control groups. In particular, the CPGC and BCP groups showed greater bone formation than the CM and control groups. Conclusions: In conclusion, CPGC was replaced rapidly with an abundant volume of new bone; CPGC also contributed slightly to regeneration of the periodontal apparatus.
참고문헌 (17)
- Parikh SN, "Bone graft substitutes in modern orthopedics", Orthopedics, 25, pp.1301~1309 (2002)
- Ahlmann E, "Comparison of anterior and posterior iliac crest bone grafts in terms of harvest-site morbidity and functional outcomes", J Bone Joint Surg Am, 84A, pp.716~720 (2002)
- Kurashina K, "Kobayashi H. Ectopic osteogenesis with biphasic ceramics of hydroxyapatite and tricalcium phosphate in rabbits", Biomaterials, 23, pp.407~412 (2002)
- Jarcho M, "Tissue, cellular and subcellular events at a bone-ceramic hydroxylapatite interface", J Bioeng, 1, pp.79~92 (1977)
- Rabalais ML, Jr, "Evaluation of durapatite ceramic as an alloplastic implant in periodontal osseous defects. I. Initial six-month results", J Periodontol, 52, pp.680~689 (1981)
- Gao H, "Effect of composition on the release kinetics of phosphate controlled release glasses in aqueous medium", J Control Release, 96, pp.21~28 (2004)
- Bagambisa FB, "Interaction of osteogenic cells with hydroxylapatite implant materials in vitro and in vivo", Int J Oral Maxillofac Implants, 5, pp.217~226 (1990)
- Kim YK, "Clinical evaluations of OSTEON as a new alloplastic material in sinus bone grafting and its effect on bone healing", J Biomed Mater Res B Appl Biomater, 86, pp.270~277 (2008)
- Hosono H, "Porous glass-ceramics composed of a titanium phosphate crystal skeleton: a review", J Non-Cryst Solids, 190, pp.185~197 (1995)
- Lee BH, "Biodegradable bone cement using calcium phosphate glass", Key Eng Mater, 309, pp.861~864 (2006)
- Nery EB, "Biphasic calcium phosphate ceramic combined with fibrillar collagen with and without citric acid conditioning in the treatment of periodontal osseous defects", J Periodontol, 61, pp.166~172 (1990)
- Bohner M, "Physical and chemical aspects of calcium phosphates used in spinal surgery", Eur Spine J, 10(Suppl 2), pp.S114~121 (2001)
- Bunker BC, "Phosphate glass dissolution in aqueous solutions", J Non-Cryst Solids, 64, pp.291~316 (1984)
- Murphy KG, "Guided tissue regeneration for the treatment of periodontal intrabony and furcation defects.A systematic review", Ann Periodontol, 8, pp.266~302 (2003)
- Dias AG, "In vitro degradation studies of calcium phosphate glass ceramics prepared by controlled crystallization", J Non-Cryst Solids, 330, pp.81~89 (2003)
- Maus U, "BMP-2 incorporated in a tricalcium phosphate bone substitute enhances bone remodeling in sheep", J Biomater Appl, 22, pp.559~576 (2008)
- Wolff KD, "Degradable injectable bone cement in maxillofacial surgery: indications and clinical experience in 27 patients", J Craniomaxillofac Surg, 32, pp.71~79 (2004)