The Effects of Hydroxyapatite-Chitosan Membrane on Bone Regeneration in Rat Calvarial Defects
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: Recently, interest in chitosan has increased due to its excellent biological properties such as biocompatibility, antibacterial effect, and rapid healing capacity. On the other hand, hydroxyapatite is used as a bone substitute in the fields of orthopedics and dentistry. The hydroxyapatite-chitosan (HA-CS) complex containing hydroxyapatite nanoparticles was developed for synergy of both biomaterials. The objective of this study was to evaluate the effect of hydroxyapatite (HA)-chitosan (CS) membrane on bone regeneration in the rat calvarial defect. Methods: Eight-millimeter critical-sized calvarial defects were created in 70 male Sprague-Dawley rats. The animals were divided into 7 groups of 10 animals and received either 1) chitosan (CS) 100% membrane, 2) hydroxyapatite (HA) 30%/CS 70% membrane, 3) HA 30%/CS 70%, pressed membrane, 4) HA 40%/CS 60% membrane, 5) HA 50%/CS 50% membrane, 6) HA 50%/CS 50%, pressed membrane, or 7) a sham – surgery control. The amount of newly formed bone from the surface of the rat calvarial defects was measured using histomorphometry, following 2- or 8- week healing intervals. Results: Surgical implantation of the HA-CS membrane resulted in enhanced local bone formation at both 2 and 8 weeks compared to the control group. The HA-CS membrane would be significantly more effective than the chitosan membrane in early bone formation. Conclusions: Concerning the advantages of biomaterials, the HA-CS membrane would be an effective biomaterial for regeneration of periodontal bone. Further studies will be required to improve the mechanical properties to develop a more rigid scaffold for the HA-CS membrane. (J Korean Acad Periodontol 2009;39:213-222)
Abstract
Purpose: Recently, interest in chitosan has increased due to its excellent biological properties such as biocompatibility, antibacterial effect, and rapid healing capacity. On the other hand, hydroxyapatite is used as a bone substitute in the fields of orthopedics and dentistry. The hydroxyapatite-chitosan (HA-CS) complex containing hydroxyapatite nanoparticles was developed for synergy of both biomaterials. The objective of this study was to evaluate the effect of hydroxyapatite (HA)-chitosan (CS) membrane on bone regeneration in the rat calvarial defect. Methods: Eight-millimeter critical-sized calvarial defects were created in 70 male Sprague-Dawley rats. The animals were divided into 7 groups of 10 animals and received either 1) chitosan (CS) 100% membrane, 2) hydroxyapatite (HA) 30%/CS 70% membrane, 3) HA 30%/CS 70%, pressed membrane, 4) HA 40%/CS 60% membrane, 5) HA 50%/CS 50% membrane, 6) HA 50%/CS 50%, pressed membrane, or 7) a sham – surgery control. The amount of newly formed bone from the surface of the rat calvarial defects was measured using histomorphometry, following 2- or 8- week healing intervals. Results: Surgical implantation of the HA-CS membrane resulted in enhanced local bone formation at both 2 and 8 weeks compared to the control group. The HA-CS membrane would be significantly more effective than the chitosan membrane in early bone formation. Conclusions: Concerning the advantages of biomaterials, the HA-CS membrane would be an effective biomaterial for regeneration of periodontal bone. Further studies will be required to improve the mechanical properties to develop a more rigid scaffold for the HA-CS membrane. (J Korean Acad Periodontol 2009;39:213-222)
참고문헌 (32)
- Magnusson I, "New attachment formation following controlled tissue regeneration using biodegradable membranes", J Periodontol, 59, pp.1~6 (1998)
- Aquirre-Zorzano LA, "Periodontal regeneration of infrabony defectsusing resorbable membrane: determinant of the healing response. An observational clinical study", Int J Periodontics Rest Dent, 19, pp.363~371 (1999)
- Zitzmann NU, "Resorbable versus nonresorbable membranes in combination with Bio-Oss for guided bone regeneration", Int J Oral Maxillofac Implant, 12, pp.844~852 (1997)
- Becker W, "A prospective multi- center study evaluation periodontal regeneration for class II furcation invasions and intrabony defects after treatment with a bioabsorbable barrier membrane: 1-year results", J Periodontol, 67, pp.641~649 (1996)
- 홍승범, "흡수성 차폐막의 조직반응에 관한 비교연구", The Journal of the Korean Academy of Periodontology (JPIS), 32(3), pp.475~488 (2002), 대한치주과학회
- Amano K, "The action of lysozyme on partially deacetylate chitin", European J Biochem, 85, pp.97~104 (1978)
- Pangburn SH, "Lysozyme degradation of partially deacetylated chitin, its films and hydrogels", Biomaterials, 3, pp.105~108 (1982)
- Reynolds, B.L., "Wound healing III: Artificial maturation of arrested regenerate with an acetylated amino sugar", The American Surgeon, 26, pp.113~117 (1960)
- 김옥수, "키토산이 치은섬유아세포에 미치는 영향", The Journal of the Korean Academy of Periodontology (JPIS), 32(1), pp.235~248 (2002), 대한치주과학회
- Paik JW, "The effects of chitosan on the human periodontal ligament fibroblasts in vitro", J Korean Acad Periodontol, 31, pp.823~832 (2001)
- Park JS, "Eight-week histological analysis on the effect of chitosan on surgically created one-wall intrabony defects in beagle dogs", J Clin Periodontol, 30, pp.443~453 (2003)
- Salata LA, "Bone healing following the use of hydroxyapatite or ionomericbone substitutes alone or combined with a guided bone regeneration technique: an animal study", Int J Oral Maxillofac Implants, 13, pp.44~51 (1998)
- Zhang C, "A study on a tissue-engineered bone using rhBMP-2 induced periosteal cells with a porous nano-hydroxyapatite", collagen, Biomed Mater, pp.2006, 2[DOI]
- Wang HL, "Utilization of a resorbable collagen membrane in repairing gingival recession defects", Pract Periodont Aesthet Dent, 8, pp.441~448 (1996)
- Wang HL, "Evaluation of and absorbale collagen membrane in treating class II furcation defects", J Periodontol, 65, pp.1029~1036 (1994)
- Polson AM, "Guided tissue regeneration in human furcation defects after using biodegradable barrier. : A multi-center feasibility study", J Periodontol, 66, pp.377~385 (1995)
- Bouchard P, "Clinical evaluation of a bioabsorble regenerative material in mandibular class II furcation therapy", J Clin Periodontol, 24, pp.511~518 (1997)
- Jung UW, "Effects of a chitosan membrane coated with polylactic and polyglycolic acid on bone regeneration in a rat calvarial defect", Biomed Mater, 3, pp.s101~s105 (2007)
- Webster TJ, "Enhanced functions of osteoblasts on nanophase ceramics", Biomaterials, 21, pp.1803~1810 (2000)
- Zhang YF, "Three-dimensional nanohydroxyapatite", chitosan scaffolds as potential tissue engineered periodontal tissue, 2007, pp.21, 333~349
- Kong L, "Preparation and characterization of nano-hydroxyapatite", chitosan composite scaffolds, 2005, pp.75, 275~282
- Guobao W, "Ma.Structure and properties of nano-hydroxyapatite", polymer composite scaffolds for bone tissue engineering, 2004, pp.25, 4749~4757
- Schmitz JP, "The critical size defect as an experimental model for craniomandibulofacial nonunoins", Clin Orthop, 205, pp.299~308 (1986)
- Caton J, "Non-human primate models for testing the efficacy and safety of periodontal regeneration procedures", J Periodontol, 65, pp.1143~1150 (1994)
- Selvig KA, "Discussion: Animal models in reconstructive therapy", J Periodontol, 65, pp.1169~1172 (1994)
- Jung UW, "The bone regenerative effects of chitosan on the calvarial critical size defect in Sprague- Dawley rats", J Korean Acad Periodontol, 30, pp.851~868 (2000)
- Pang EK, "Effects of chitosan on human periodontal ligament fibroblasts in vitro and on bone formation in rat calvarial defects", J Periodontol, 76, pp.1526~1533 (2005)
- Oonishi H, "Comparative bone growth behavior in granules of bioceramic materials of various sizes", J Biomed Mater Res, 44, pp.31~43 (1999)
- Manjubala I, "Bioactivity and osseointegration study of calcium phosphate ceramic of different chemical composition", J Biomed Mater Res, 63, pp.200~208 (2002)
- Liljensten E, "Resorbable and nonresorbable hydroxyapatite granules as bone graft substitutes in rabbit cortical defects", Clin Implant Dent Relat Res, 5, pp.95~101 (2003)
- Andrade JC, "Behavior of dense and porous hydroxyapatite implants and tissue response in rat femoral defects", J Biomed Mater Res, 62, pp.30~36 (2002)
- Takeshita F, "Histological comparison of early wound healing following dense hydroxyapatite granule grafting and barrier placement in surgically-created bone defects neighboring implants", J Periodontol, 68, pp.924~932 (1997)