망간이 백서 두개관세포에 미치는 영향
Effect of Manganese on primary rat calvarial cell
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Abstract
Chronic exposure to high levels of manganese leads a pronounce and debilitating disorder known as manganism. Research on the toxic manifestation of manganese have focused primarily on its neurological effects because exposure to high levels of the metal produces a distinct and irreversible extrapyramidal dysfunction resembling the dystonic movements associated with Parkinson's physiological and biochemical systems in the body. The purpose of this study was to evaluate the effect of manganeses on primary rat calvarial cell growth and toxicity. The experimental groups were in concentration of 0, 10, 30, 60, 100, 300 μ M. Cell activity was assessed at day 1 and day 3 using a fluorescent molecular probe. Cell proliferation was evaluated at day 1 and day 3 by MTT assay. The amount of total protein synthesis was measured at day 3 and day 7. The results were as follows: The proliferation of primary rat calvarial cells were inhibited by MnCl2 in the concentration exceeding 100μM. The primary rat calvarial cells treated with MnCl2 showed similar protein synthesis to the control group except in 100 μM. These result suggest that manganese suppress the viability and protein synthesis of primary rat calvarial cells in concentration exceeding 100μM.
참고문헌 (25)
- Petersdorf RG, "Harrison's principles of internal medicine. McGraw Hill", pp.443~445 (1991)
- "Manganese. National Academy of science. III" (1973)
- Verity MA, "Manganese neurotoxicity; A mechanism hypothesis", 20, pp.489~497 (1999)
- The major Mn, "enzyme in mammalian brain. curr Top Cell Regul 24", pp.153~69 (1984)
- Domingo JL, "Maternal and develomental toxicity of manganese in the mouse. Toxicology Letters 69", pp.45~52 (1993)
- Donaldson J, "The physiopathologic significance of manganese in brain its relation to schizoprenia and neurodegenerative disorders", pp.451~8 462 (1987)
- Torrente M, "Interactions in developmental toxicology: effects of combined administration of manganese and hydrocortisone", 17, pp.173~179 (2000)
- Burkhard PR, "Chronic parkinsonism associated with cirrhosis a distinct subset of acquired hepatocerebral degeneration", 60, pp.521~528 (2003)
- Anderson M, "Mn ions pass through calcium channels", pp.805~827 (1983)
- Roth JA, "Mechanism of manganese-induced rat pheochromocytoma (PC12) cell death and cell differentiation", 23, pp.147~157 (2002)
- Roth JA, "Manganese-induced rat pheochromocytoma (PC12) cell death is independent of caspase activation", 61, pp.162~171 (2000)
- Kita H, "Mn and Mg influxes through calcium channels of motor nerve terminals are prevented by verapamil in frogs. Brain Res 510", pp.289~295 (1990)
- Choi DW, "Excitotoxic cell death", 23, pp.1261~1276 (1992)
- Gunter KK, "Manganese and calcium efflux kinetics in brain mitochondria. Biochem J 266", pp.329~334 (1990)
- Bernasconi S, "Role of manganese in MG-63 osteosarcoma cell attachment to fibrinogen and von willebrand factor", 65, pp.96~103 (1991)
- Centrella M, "Further biochemical and molecular characterization of primary rat parietal bone cell culture", pp.401~408 (1988)
- I, "Some trace elements which are essential for human nutrition Progress in Food and Nutrition Science", pp.371~391 (1975)
- New York, "Trace element in human and animal Nutrition" (1971), Academic Press
- Romano MA, "Nramp2 is mutated in the anemic Belgrade (b) rat; evidence of a role for Nramp2 in endosomal iron transport", 95, pp.1148~1153 (1998)
- Mackenzie B, "Cloning and characterization of a mammalian proton-coupled metal-ion transporter", 388, pp.482~488 (1997)
- Olatunbosun D, "Interrelation of intestinal transport system for manganese and iron", 78, pp.642~655 (1971)
- Sandri G, "Transport of Ca2+ and Mn2+ by mitochondria from rat liver", (rdenbrand k), pp.1241~67 1250 (1985)
- Grahm DG, "A proposal for molecular pathogenesis of manganese neurotoxicity and Parkinson's disease", pp.83~5 96 (1984)
- Adachi K, "Activation of JNK pathway and induction of apoptosis by manganese in PC12 cells", 71, pp.1607~1615 (1998)
- Martin SJ, "Apoptosis and cancer; the failure of controls on cell death and cell survival", 18, pp.137~153 (1995)