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랫드의 두개골세포 활성 및 mRNA 발현에 미치는 홍화자의 영향

Effects of Carthami Tinctorius on Rat Calvarial Cell Activity and MG63 Cell mRNA Synthesis

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참고문헌

주제분류한의학

초록

Abstract

This study was performed to evaluate the effect of Carthami tinctorius(HH) on osteoblast function and gene expression. The osteoblasts separated from the rat calvariae were cultivated to evaluate the cell function, and MG-63 cell was also cultivated for the test of mRNA synthesis. In this experiments, cell proliferation of rat calvarial cells was increased by HH. PKC activity, intracellular free calcium level and collgen synthesis from calvarial cells were increased by HH, but not PKA activity. And the mRNA of PLA2, COX-2, and PGE2 synthase from MG-63 were decreased by HH, but the mRNA of prostacyclin synthase was increased. It is concluded that HH might increase the proliferation of calvarial celll resulted from augumentation of osteoblast activity and its mRNA synthesis.

참고문헌 (20)

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