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키토산이 백서 태자두개관세포의 세포외기질 발현과 석회화에 미치는 영향

Effect of chitosan on bone matrix expression and mineralization in primary rat calvarial cell

최득철|김영준|정현주|김옥수|김재철
대한치주과학회 (The Korean Academy Of Periodontology)|2004.12|Journal of Periodontal & Implant Science|제34권 제4호|pp. 759-769

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참고문헌

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초록

Abstract

Periodontal therapy has dealt primarily with attempts at arresting progression of disease, however, more recent techniques have focused on regenerating the periodontal ligament having the capacity to regenerate the periodontium. The effect of chitosan, a carbohydrate biopolymer extracted from chitin, on periodontal ligament regeneration is of particular interest. The purpose of this study was to evaluate the effect of chitosan on the expression of extracellular matrix proteins in primary rat calvarial cells in vitro. In the control group, cells was cultured with BGJb media. In the experimental groups, cells were cultured with chitosan in concentration of 0.01, 0.1, 1.0 and 2.0 ㎎/㎖. Then each group was characterized by examining alkaline phosphatase activity at 3 and 7 days, and the ability to produce mineralized nodules of rat calvarial cells at 14 and 21 days. Synthesis of type I collagen (COL-I), osteocalcin (OCN), bone sialoprotein (BSP) was evaluated by RT-PCR at 14 days. The results were as follows: 1. Alkaline phosphatase activity was significantly higher in the concentration of chitosan 0.01㎎/㎖, 0.1㎎/㎖ and 1.0㎎/㎖ compared to control (p<0.05). 2. The percentage of mineralized bone nodule was more in the concentration of chitosan 0.1㎎/㎖ and 1.0㎎/㎖ than the control. 3. At 14 day culture, the expression of OCN was increased by chitosan in concentration of 1.0 ㎎/㎖ and 2.0 ㎎/㎖. These results suggested that chitosan in concentration of 0.1 and 1.0 ㎎/㎖ stimulate the extracellular matrix of primary rat calvarial cells and may facilitate the formation of bone.

참고문헌 (32)

  1. "On the repair potential of periodontal tissue. J Periodontol 1976", pp.256~260
  2. "Progenitor cell kinetics during tissue regeneration in experimental periodontal wounds. J Periodont Res 1988", pp.107~117
  3. "Applications and environmental aspects of chitin and chitosan", 32, pp.629~640 (1995)
  4. "Role of fibroblast subpopulations in periodontal physiology and pathology. J Periodont Res 1991", pp.144~154
  5. "Chitin and chitosan in the polysaccharides", pp.386~390 (1983), Academic press
  6. "Biological activity of chitosan", 9, pp.247~252 (1988)
  7. "Regeneration of the connective tissue attachment on surgically exposed roots using a fibrin-fibronectin adhesive system. J Periodont Res 1987", pp.320~326
  8. "Clinical evaluation of freeze-dried bone allografts in periodontal osseous defects. J Periodontol 1976", pp.125~131
  9. "Commercial uses and potential applications In Chitin and Chitosan", pp.51~70 (1989)
  10. "The discovery of a potent pure chemical wound-healing accelerator", 119, pp.560~564 (1970)
  11. "Collagen membranes prevent apical migration of epithelium and support new connective tissue attachment during periodontal wound healing in dogs. J Periodontal Res 1989", (ff m), pp.247~253
  12. "potential role of bone morphogenic proteins. J Periodont Res 1994", pp.225~235
  13. "A new topical agent for diffuse capillary bleeding in brain tissue", pp.9~13
  14. "Evidence for up-regulation of epidermal growth factors on rat periodontal ligament fibroblastic cells associated with stabilization of phenotype in vitro. Arch Oral Biol 1993", pp.559~569
  15. "Effect of chitosan on lingual hemostasis in rabbits", 49, pp.858~863 (1991)
  16. "Chitosan in dentistry Chitin in Nature and Technology", pp.507~512 (1986)
  17. "Bone formation by osteclast-like cells in a three-dimension cell culture. Calcif Tissue Int 1990", pp.46~56
  18. "Reconstruction of periodontal tissue with chitosan", 14, pp.39~43 (1993)
  19. "Chitosan과 chitosan-cellulose를 이용한 차폐막의 골조직 재생유도능력에 관한 연구", 28, pp.611~630 (1998)
  20. "Further biochemical and molecular characterization of primary rat parietal bone cell culture", 3, pp.401~408 (1988)
  21. "In vitro formation of mineralized nodules by periodontal ligament cells from the rat", 50, pp.459~467 (1992)
  22. "Synthesis of cementum-like tissue in vitro by cells cultured from bone", pp.592~612
  23. "Chemical and physical property modifications induced by ion irradiation inpolymers. In: Material and Processes for surface and interface engineering", 290, pp.597~640 (1995)
  24. "Chitin in nature and technology", pp.435~442 (1986), Pleum Press
  25. "Osteogenesis enhanced by chitosan in vitro", 67, pp.1170~1175 (1996)
  26. "Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes", 15(51), pp.586~595 (2000)
  27. "키토산이 치주인대 섬유아세포에 미치는 영향", 31, pp.823~832 (2001)
  28. "Calcification of dentinal collagen by cultured rabbit periodontium The role of alkaline phosphatase", pp.159~171
  29. "Mechanism of mineral formation in bone", 60, pp.320~330 (1989)
  30. "The possible roles of osteocalcin osteopontin and bone sialoprotein in bone mineralization and resorption", pp.617~628, Why does bone matrix contain non-collagenous proteins
  31. "A role for osteocalcin in osteoblast differentiation", 45, pp.292~302 (1991)
  32. "Osteoconductive properties of methylpytrrolidine chitosan in an animal model", 14, pp.925~929 (1993)

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