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키토산이 백서 태자 두개관세포에 미치는 영향

Effect of chitosan in primary rat calvarial cell

정현주|김영준|김옥수|김정경
대한치주과학회 (The Korean Academy Of Periodontology)|2004.12|Journal of Periodontal & Implant Science|제34권 제4호|pp. 747-757

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참고문헌

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초록

Abstract

The effect of chitosan, a carbohydrate biopolymer extracted from chitin, on periodontal regeneration is of particular interest. The purpose of this study was to evaluate the effect of chitosan on primary rat calvarial cells in vitro, with special focus on their proliferative properties by cell activity and the amount of total protein synthesis. The experimental groups were cultured with chitosan in concentration of 0.01, 0.1, 1.0, 2.0 and 5.0 ㎎/㎖ for MTT assay. In the experimental groups, cells were cultured with chitosan in concentration of 0.01, 0.1, 1.0 and 2.0 ㎎/㎖. Each group was characterized by examining alkaline phosphatase activity at 3 and 7 days and the ability to produce mineralized nodules of rat calvarial cells at 14 and 21 days. The results were as follows: 1. The cell activity was not reduced in the concentration of 0.01∼1.0 ㎎/㎖ whereas the cell activity was reduced in the concentration of 5.0 ㎎/㎖ than the control at day 1 and 3 (p<0.05). 2. Primary rat calvarial cells treated with chitosan in the concentration 0.01 ㎎/㎖ and 0.1 ㎎/㎖ showed more protein synthesis than the control at day 3 (p<0.01). But primary rat calvarial cells treated with chitosan showed more protein synthesis than in control but they didn't have statistically difference among groups at day 7. 3. At 3 and 7 days, alkaline phosphatase activity was significantly increased in the concentration of 0.01 ㎎/㎖. 0.1 ㎎/㎖ and 1.0 ㎎/㎖ (p<0.05). 4. The percentage of mineralized bone nodule was more in the concentration of chitosan 0.1 ㎎/㎖ and 1.0 ㎎/㎖ than the control. These results suggested that chitosan has a positive effect on the bone formation of primary rat calvarial cells in the concentration of 0.1 ㎎/㎖ and 1.0 ㎎/㎖

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관련 논문AI추천

키토산이 백서 태자두개관세포의 세포외기질 발현과 석회화에 미치는 영향김영준, 김옥수, 김재철, 정현주, 최득철대한치주과학회 · Journal of Periodontal & Implant Science2004 · 제34권 제4호키토산이 조골세포에 미치는 영향김기홍, 박영주, 박준우, 안병근, 이용찬, 조병욱대한구강악안면외과학회 · 대한구강악안면외과학회지2002 · 제28권 제4호키토산이 치은섬유아세포에 미치는 영향김옥수, 정현주대한치주과학회 · Journal of Periodontal & Implant Science2002 · 제32권 제1호백서두개골 결손부에서 키토산/흡수성 콜라겐 전달체의 골재생김수경, 김종관, 김창성, 석헌주, 조규성, 채중규, 최성호대한치주과학회 · Journal of Periodontal & Implant Science2003 · 제33권 제3호망간이 백서 두개관세포에 미치는 영향김영준, 김옥수, 김지은, 정현주대한치주과학회 · Journal of Periodontal & Implant Science2004 · 제34권 제2호망간이 백서 두개관세포의 골형성능에 미치는 영향김영준, 김옥수, 정현주, 최득철, 한웅택대한치주과학회 · Journal of Periodontal & Implant Science2004 · 제34권 제4호

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