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법랑기질유도체가 탈회 치근표면에서 치주인대섬유아세포의 생물학적 성상에 미치는 영향

Effects of enamel matrix derivatives on biologic activities of human periodontal fibloblasts to demineralized root surface

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초록

Purpose: The aim of this study was to investigate the effects of EMD on demineralized root surface using human periodontal ligament cells and compare the effects of root conditioning materials(tetracycline(TCN), EDTA). Material and Methods: Dentin slices were prepared from the extracted teeth and demineralized with TCN and EDTA. Demineralized dentin slices were incubated at culture plate with 25, 50 and 100 ㎍/ml concentration of EMD. Cell attachment, alkaline phosphatase activity test, protein synthesis assay and scanning electronic microscopic examination were done. Results: Cells were attached significantly higher in EMD treated group at 7 and 14 days. Cell numbers were significantly higher in EMD treated group. Alkaline phosphatase activity was significantly higher in EMD treated group at 7 and 14 days. Protein synthesis was significantly higher in EMD treated group at 7 and 14 days. Conclusion: Enamel matrix derivatives enhance the biologic activities of human periodontal ligament cells on demineralized root surface and its effects are dependent on the concentration of EMD. (J Korean Acad Periodontol 2008;38:679-690)

Abstract

Purpose: The aim of this study was to investigate the effects of EMD on demineralized root surface using human periodontal ligament cells and compare the effects of root conditioning materials(tetracycline(TCN), EDTA). Material and Methods: Dentin slices were prepared from the extracted teeth and demineralized with TCN and EDTA. Demineralized dentin slices were incubated at culture plate with 25, 50 and 100 ㎍/ml concentration of EMD. Cell attachment, alkaline phosphatase activity test, protein synthesis assay and scanning electronic microscopic examination were done. Results: Cells were attached significantly higher in EMD treated group at 7 and 14 days. Cell numbers were significantly higher in EMD treated group. Alkaline phosphatase activity was significantly higher in EMD treated group at 7 and 14 days. Protein synthesis was significantly higher in EMD treated group at 7 and 14 days. Conclusion: Enamel matrix derivatives enhance the biologic activities of human periodontal ligament cells on demineralized root surface and its effects are dependent on the concentration of EMD. (J Korean Acad Periodontol 2008;38:679-690)

참고문헌 (30)

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관련 논문AI추천

Effects of enamel matrix derivatives on the proliferation and the release of growth factors of human periodontal ligament cells방은경, 정겨운대한치과보철학회 · 대한치과보철학회지2016 · 제54권 제3호법랑기질 단백질 유도체가 치주인대양세포 및 조골양세포에 미치는 영향고선일, 김동운, 임성빈, 정진형대한치주과학회 · Journal of Periodontal & Implant Science2003 · 제33권 제2호법랑기질유도체가 치주인대세포, 불멸화 조골세포, 백악질 유래세포의 증식과 유전자 발현에 미치는 영향구영, 김경화, 김태일, 류인철, 설양조, 이용무, 이해준, 정유지, 정종평, 한수부대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제2호치주염에 이환되어 발거된 치아의 치주인대가 없는 치근 표면에 EDTA처리를 한 후 3차원적으로 배양한 사람치주인대섬유모세포의 부착에 관한 주사전자현미경적 연구김병옥, 김흥중, 박주철, 장현선, 조아영대한치주과학회 · Journal of Periodontal & Implant Science2007 · 제37권 제1호Enamel Matrix Derivatives가 사람 치주인대 세포의 특이유전자인 PDLs17, PDLs22의 발현에 끼치는 효과국중기, 김병옥, 김종관, 김흥중, 박주철, 장현선, 한근아대한치주과학회 · Journal of Periodontal & Implant Science2004 · 제34권 제2호표피성장인자가 치주인대 섬유 모세포의 증식과부착에 미치는 영향김성오, 김연태, 김종관, 이종은, 최형준대한소아치과학회 · 대한소아치과학회지2005 · 제32권 제3호

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