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Enamel Matrix Derivatives가 사람 치주인대 세포의 특이유전자인 PDLs17, PDLs22의 발현에 끼치는 효과

Effect of Enamel Matrix Drivatives application on the expression of PDLs17, PDLs22 of cultured human periodontal ligament cells in vitro

김흥중|국중기|김종관|장현선|박주철|김병옥|한근아
대한치주과학회 (The Korean Academy Of Periodontology)|2004.06|Journal of Periodontal & Implant Science|제34권 제2호|pp. 333-344

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Abstract

The enamel matrix derivative (EMD) has been recently used in the periodontal regenerative techniques. The present study was established to investigate the influence of EMD on human periodontal ligament cells using expression of mRNA of periodontal ligament specific gene (PDLs)17, PDLs22, type I collagen when EMD applied to periodontal ligament cells. Periodontal ligament cells were obtained from a healthy periodontium and cultured in Dulbecco's modified Eagle's medium (DMEM) plus 10% fetal bovine serum and β -glycerophosphate with ascorbic acid. Test groups were two; One adds EMD in culture media and another added EMD and Dexamethasone (DEX) in culture media. Positive control group added DEX in culture media, and negative control group adds niether of EMD nor DEX. EmdogainⓇ (Biora, Sweden, 30 ㎎/㎖) was diluted by 75 ㎍/㎖ concentration to culture media. For reverse transcription-polymerase chain reaction (RT-PCR), total RNA isolated on days 0, 7, 14 and 21. mRNA of PDLs17 was expressed on days 14 and 21 in EMD or DEX group, and expressed on days 7, 14 and 21 in EMD plus DEX group, the other side, expressed on days 21 in negative control group. mRNA of PDLs22 expressed on days 7, 14 and 21 in EMD group, and expressed on days 14 and 21 in DEX group, and expressed on days 7, 14 and 21 in EMD plus DEX group. Negative control group expressed on days 14 and 21. Type I collagen was expressed on all days and all groups. These results indicate that EMD promotes differentiation of periodontal ligament cells, and this is considered to offer basis that can apply EMD to periodontal tissue regeneration technique.

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관련 논문AI추천

법랑기질 단백질 유도체가 치주인대양세포 및 조골양세포에 미치는 영향고선일, 김동운, 임성빈, 정진형대한치주과학회 · Journal of Periodontal & Implant Science2003 · 제33권 제2호고농도 포도당 환경에서 EMD(Enamel Matrix Derivatives)가 인간 치주인대 세포와 뼈모세포양 세포(MC3T3-E1)에 미치는 영향권용대, 김선욱, 이백수, 주성숙대한구강악안면외과학회 · 대한구강악안면외과학회지2008 · 제34권 제5호Effects of enamel matrix derivatives on the proliferation and the release of growth factors of human periodontal ligament cells방은경, 정겨운대한치과보철학회 · 대한치과보철학회지2016 · 제54권 제3호배양된 치주인대세포와 치은섬유아세포에서 상이하게 발현된 유전자들의 검토 양상김문규, 김정철, 박진우, 서조영, 여신일, 윤혜정, 최미혜, 최병주대한치주과학회 · Journal of Periodontal & Implant Science2006 · 제36권 제3호치주인대세포와 치은섬유아세포의 분화에 미치는 rhBMP-2의 효과에 대한 연구구영, 김경화, 김현종, 류인철, 이용무, 정종평, 최상묵, 한수부대한치주과학회 · Journal of Periodontal & Implant Science2002 · 제32권 제2호법랑기질유도체가 치주인대세포, 불멸화 조골세포, 백악질 유래세포의 증식과 유전자 발현에 미치는 영향구영, 김경화, 김태일, 류인철, 설양조, 이용무, 이해준, 정유지, 정종평, 한수부대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제2호

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