Effects of enamel matrix derivatives on the proliferation and the release of growth factors of human periodontal ligament cells
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Purpose: Stimulating the proliferation and migration of periodontal ligament cells (PDLCs) has become the main goal of periodontal regeneration. To accomplish this goal, regeneration procedures have been developed, but results have not been predictable. Recently, tissue engineering using enamel matrix derivatives (EMDs) and growth factors has been applied to periodontal regeneration; however, the mechanism of EMDs is largely unknown. The aim of this study was to investigate the effects of EMDs on the proliferation and release of growth factors from PDLCs. Materials and methods: Human PDLCs were removed from individually extracted 3rd molars of healthy young adults, and cultured in the media containing EMDs (Emdogain, Biora, Malmo, Sweden) at concentration of 0, 12.5, 25, 50, 100, and 200 μg/mL each. Cell proliferation and ALP (alkaline phosphatase) activity were measured. The evaluation of growth factors released by PDLCs was also performed by one-way analysis of variance (ANOVA) and Bonferroni's multiple comparison test. Results: Significantly increased proliferation and ALP activity were observed in PDLCs treated with over 25 μg/mL and 50 μg/mL EMDs, respectively. Additionally, treatment of PDLCs with 50 μg/mL resulted in significantly increased release of vascular endothelial growth factor (VEGF) and transforming growth factor (TGF)-βafter 24 h and 48 h, respectively. Conclusion: EMDs enhance the proliferation and ALP activity of PDLCs, and promote the release of growth factors, includingVEGF and TGF-β, from PDLCs. Therefore EMDs could be one of the effective methods for periodontal regeneration.
Abstract
Purpose: Stimulating the proliferation and migration of periodontal ligament cells (PDLCs) has become the main goal of periodontal regeneration. To accomplish this goal, regeneration procedures have been developed, but results have not been predictable. Recently, tissue engineering using enamel matrix derivatives (EMDs) and growth factors has been applied to periodontal regeneration; however, the mechanism of EMDs is largely unknown. The aim of this study was to investigate the effects of EMDs on the proliferation and release of growth factors from PDLCs. Materials and methods: Human PDLCs were removed from individually extracted 3rd molars of healthy young adults, and cultured in the media containing EMDs (Emdogain, Biora, Malmo, Sweden) at concentration of 0, 12.5, 25, 50, 100, and 200 μg/mL each. Cell proliferation and ALP (alkaline phosphatase) activity were measured. The evaluation of growth factors released by PDLCs was also performed by one-way analysis of variance (ANOVA) and Bonferroni's multiple comparison test. Results: Significantly increased proliferation and ALP activity were observed in PDLCs treated with over 25 μg/mL and 50 μg/mL EMDs, respectively. Additionally, treatment of PDLCs with 50 μg/mL resulted in significantly increased release of vascular endothelial growth factor (VEGF) and transforming growth factor (TGF)-βafter 24 h and 48 h, respectively. Conclusion: EMDs enhance the proliferation and ALP activity of PDLCs, and promote the release of growth factors, includingVEGF and TGF-β, from PDLCs. Therefore EMDs could be one of the effective methods for periodontal regeneration.
참고문헌 (18)
- Hoang AM, "In vitro wound healing responses to enamel matrix derivative", J Periodontol, 71, pp.1270~1277 (2000)
- Chong CH, "Human periodontal fibroblast response to enamel matrix derivative, amelogenin, and platelet-derived growth factor-BB", J Periodontol, 77, pp.1242~1252 (2006)
- Slavkin HC, "Towards a cellular and molecular understanding of periodontics. Cementogenesis revisited", J Periodontol, 47, pp.249~255 (1976)
- Gestrelius S, "In vitro studies on periodontal ligament cells and enamel matrix derivative", J Clin Periodontol, 24, pp.685~692 (1997)
- Hammarström L, "Enamel matrix, cementum development and regeneration", J Clin Periodontol, 24, pp.658~668 (1997)
- Heijl L, "Enamel matrix derivative (EMDOGAIN) in the treatment of intrabony periodontal defects", J Clin Periodontol, 24, pp.705~714 (1997)
- Froum SJ, "A comparative study utilizing open flap debridement with and without enamel matrix derivative in the treatment of periodontal intrabony defects:a 12-month re-entry study", J Periodontol, 72, pp.25~34 (2001)
- Sculean A, "Treatment of intrabony defects with enamel matrix proteins or bioabsorbable membranes. A 4-year follow-up split-mouth study", J Periodontol, 72, pp.1695~1701 (2001)
- Van der Pauw MT, "Enamel matrix-derived protein stimulates attachment of periodontal ligament fibroblasts and enhances alkaline phosphatase activity and transforming growth factor beta1 release of periodontal ligament and gingival fibroblasts", J Periodontol, 71, pp.31~43 (2000)
- Melcher AH, "On the repair potential of periodontal tissues", J Periodontol, 47, pp.256~260 (1976)
- Boyan LA, "Mitogenic and chemotactic responses of human periodontal ligament cells to the different isoforms of platelet-derived growth factor", J Dent Res, 73, pp.1593~1600 (1994)
- Lyngstadaas SP, "Autocrine growth factors in human periodontal ligament cells cultured on enamel matrix derivative", J Clin Periodontol, 28, pp.181~188 (2001)
- Grandin HM, "Enamel matrix derivative:a review of cellular effects in vitro and a model of molecular arrangement and functioning", Tissue Eng Part B Rev, 18, pp.181~202 (2012)
- Schlueter SR, "In vitro effects of enamel matrix derivative on microvascular cells", J Periodontol, 78, pp.141~151 (2007)
- Mirastschijski U, "Effects of a topical enamel matrix derivative on skin wound healing", Wound Repair Regen, 12, pp.100~108 (2004)
- Pfeilschifter J, "Stimulation of bone matrix apposition in vitro by local growth factors: a comparison between insulin-like growth factor I, platelet-derived growth factor, and transforming growth factor beta", Endocrinology, 127, pp.69~75 (1990)
- Gao J, "Expression of transforming growth factor-beta receptors types II and III within various cells in the rat periodontium", J Periodontal Res, 34, pp.113~122 (1999)
- Hobbs HC, "Periodontal ligament cells from insulin-dependent diabetics exhibit altered alkaline phosphatase activity in response to growth factors", J Periodontol, 70, pp.736~742 (1999)