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표피성장인자가 치주인대 섬유 모세포의 증식과부착에 미치는 영향

The effects of EGF on proliferation and attachment of humanperiodontal fibroblasts

김종관|최형준|김성오|이종은|김연태
대한소아치과학회 (The Korean Academy Of Pediatric Dentistry)|2005.09|대한소아치과학회지 (Journal of the Korean academy of pediatric dentistry)|제32권 제3호|pp. 395-402

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키워드

EGF표피성장인자섬유결합소치주인대 섬유모세표치아 완전탈구FibronectinPDL fibroblastsTooth avulsion14
주제분류치의학

초록

손상 받은 치주 조직의 치유과정은 치주인대 섬유 모세포의 세포 활성도에 영향을 받는다. 또한 치유과정 중 치주인대 섬유모세포간의 재부착이 이루어져야 한다. 본 연구의 목적은 표피성장인자가 치주인대 세포의 증식과 부착에 미치는 영향을 알아보는 것으로 이를 바탕으로 향후 완전 탈구된 치아의 보관용액이나 재식전 처리제로서의 표피성장인자의 효용성을 고찰해보기 위함이다. 발치된 사람의 제 1소구치에서 치주인대 섬유모세포를 채취 및 배양한 후, 세포독성을 및 세포의 최고 활성도를 평가 하기 위해 MTT assay를 시행하였다. 표피성장인자가 첨가된 실험군과 대조군의 세포증식의 차이를 비교하였고, western blot을 통해서 세포 부착에 관여하는 섬유결합소의 발현을 실험군과 대조군을 통해 비교하여 다음의 결과를 얻었다. 표피성장인자는 치주인대 섬유모세포에 대하여 세포독성을 보이지 않으며, 최고의 활성도를 보이는 농도는 10ng/㎖였다. 또한 치주인대 섬유모세포의 배지내 증식정도는 10ng/㎖의 실험군에서 대조군에 비해 유의하게 높았다. 세포간 부착에 관여하는 섬유결합소의 발현율이 실험군에서 대조군에 비해 유의하게 증가하였다. 본 연구를 통해 표피성장인자는 치주인대 섬유모세포의 재생을 촉진하며 따라서 완전 탈구된 치아의 보관용액이나 재식전 처리제로 사용될 수 있음을 시사한다.

Abstract

In considering the healing process of injured periodontal tissue, healing rate would be influenced by the cellular activity of periodontal fibroblasts (PDLFs). In addition, the reattachment among PDLFs should be induced for healing process. The purpose of this study was to evaluate the effects of epidermal growth factor(EGF) on the proliferation and attachment of PDLFs and to verify the efficacy of EGF as a storage media or a pre-replantation conditioner of traumatically avulsed tooth. Human recombinant epidermal growth factor(hrEGF) and human periodontal fibroblasts from first premolar were prepared. At first, MTT assay was done to evaluate the toxic effect on human periodontal fibroblast and the maximum cellular growth of EGF. Cellular proliferation rate was then compared between control group and 10ng/ml EGF added group. Also, western blot was done to evaluate the expression of fibronectin in both groups. The results were as follows: 1. From MTT assay, EGF showed no toxic effect on PDL fibroblasts. The highest proliferation was shown at 10ng/ml EGF. 2. In 10ng/ml EGF added group, the degree of proliferation of PDLFs was significantly higher than that in control group. 3. Fibronectin expression of EGF added group was also significantly higher than that of control group. From this study we could conclude that EGF enhanced the regeneration rate of periodontal fibroblast, which could be used as a pretreatment agent or a storage media for traumatically avulsed teeth.

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EGF, T3, HB-EGF 가 치주인대섬유모세포에 미치는 영향김성오, 김연태, 차정헌, 최병재, 홍은경대한소아치과학회 · 대한소아치과학회지2007 · 제34권 제3호법랑기질유도체가 탈회 치근표면에서 치주인대섬유아세포의 생물학적 성상에 미치는 영향구영, 김태일, 류인철, 설양조, 이강운, 이용무, 정종평, 한수부대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제4호Effects of enamel matrix derivatives on the proliferation and the release of growth factors of human periodontal ligament cells방은경, 정겨운대한치과보철학회 · 대한치과보철학회지2016 · 제54권 제3호법랑기질유도체가 치주인대세포, 불멸화 조골세포, 백악질 유래세포의 증식과 유전자 발현에 미치는 영향구영, 김경화, 김태일, 류인철, 설양조, 이용무, 이해준, 정유지, 정종평, 한수부대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제2호Mineral trioxide aggregate (MTA)가 치주인대 섬유아세포에서 분비되는 cytokine과 성장인자 TGF- β1, FGF-2 발현에 미치는 영향강명회, 권지윤, 배광식, 백승호, 이우철, 임성삼대한치과보존학회 · Restorative Dentistry and Endodontics2007 · 제32권 제3호Effect of fibroblast growth factor on injured periodontal ligament and cementum after tooth replantation in dogs김병옥, 박주철, 유상준, 이중석, 정의원, 최성호대한치주과학회 · Journal of Periodontal & Implant Science2015 · 제45권 제3호

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