Interaction between odontoblast and bio-calcium phosphate cement reinforced with chitosan
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Purpose: Calcium phosphate cement (CPC) is one of many useful materials for restoring tooth defects, periodontium and maxillofacial area. Chitosan is a biodegradable material that has been shown to promote the growth and differentiation of osteoblasts in culture. This study examined the interaction between odontoblasts and bio-calcium phosphate cement reinforced with chitosan. Materials and Methods: 5×10^3 odontoblastic cells were seeded into each well. Various concentrations of bio-calcium phosphate cement reinforced with chitosan (10, 20, 50, 100, 200, 500 μg/ml, 1, 2, 4 mg/ml) were diluted and added to the wells. The well was incubated for 24 h, 48 h and 72 h. After incubation, the number of cells was assessed to determine the cell viability. A cytokinesis-block micronucleus assay and chromosomal aberration test were carried out to estimate the extent of chromosomal abnormalities. Microscopic photographs and RT-PCR were performed to examine the adhesion potential of bio-calcium phosphate cement reinforced with chitosan. Results: Bio-CPC-reinforced chitosan did not show significant cytotoxicity. The number of damaged chromosomes in the cells treated with Bio-CPC-reinforced chitosan was similar to that in the control cells. There was no significant increase in the number of chromosomal aberrations in the Bio-CPC reinforced chitosan exposed cells. Microscopic photographs and RT-PCR confirmed the adhesive potential of bio-CPC reinforced chitosan to odontoblasts. Conclusion: Bio-CPC-reinforced chitosan did not affect the odontoblastic cell viability, and had no significant cytotoxic effect. Bio-CPC-reinforced chitosan showed adhesive potential to odontoblasts. These results are expected form the basis of future studies on the effectiveness of dental restorative materials in Bio-CPC reinforced with chitosan.
Abstract
Purpose: Calcium phosphate cement (CPC) is one of many useful materials for restoring tooth defects, periodontium and maxillofacial area. Chitosan is a biodegradable material that has been shown to promote the growth and differentiation of osteoblasts in culture. This study examined the interaction between odontoblasts and bio-calcium phosphate cement reinforced with chitosan. Materials and Methods: 5×10^3 odontoblastic cells were seeded into each well. Various concentrations of bio-calcium phosphate cement reinforced with chitosan (10, 20, 50, 100, 200, 500 μg/ml, 1, 2, 4 mg/ml) were diluted and added to the wells. The well was incubated for 24 h, 48 h and 72 h. After incubation, the number of cells was assessed to determine the cell viability. A cytokinesis-block micronucleus assay and chromosomal aberration test were carried out to estimate the extent of chromosomal abnormalities. Microscopic photographs and RT-PCR were performed to examine the adhesion potential of bio-calcium phosphate cement reinforced with chitosan. Results: Bio-CPC-reinforced chitosan did not show significant cytotoxicity. The number of damaged chromosomes in the cells treated with Bio-CPC-reinforced chitosan was similar to that in the control cells. There was no significant increase in the number of chromosomal aberrations in the Bio-CPC reinforced chitosan exposed cells. Microscopic photographs and RT-PCR confirmed the adhesive potential of bio-CPC reinforced chitosan to odontoblasts. Conclusion: Bio-CPC-reinforced chitosan did not affect the odontoblastic cell viability, and had no significant cytotoxic effect. Bio-CPC-reinforced chitosan showed adhesive potential to odontoblasts. These results are expected form the basis of future studies on the effectiveness of dental restorative materials in Bio-CPC reinforced with chitosan.
참고문헌 (27)
- Pereira JC, "Human pulpal response to direct pulp capping with an adhesive system", Am J Dent, 13, pp.139~147 (2000)
- Cox CF, "Biocompatibility of primer, adhesive and resin composite systems on non-exposed and exposed pulps of non-human primate teeth", Am J Dent, 11, pp.S55~S63 (1998)
- Machida Y, "Use of chitosan and hydroxypropylchitosan in drug formulations to effect sustained release", Drug Dis Deliv, 1, pp.119~130 (1986)
- Muzzarelli RAA, "Osteoconduction exerted by methylpyrrolidinone chitosan used in dental surgery", Biomaterials, 14(1), pp.39~43 (1993)
- Muzzarelli RAA, "Amphoteric derivatives of chitosan and their biological significance. In Chitin and chitosan", pp.87~99 (1989), Elsevier
- Yamaguchi I, "Precipitation and microstructure analysis of chitosan", hydroxyapatite nanocomposites, 2001, pp.55, 20~27
- LeGeros RZ, "Biodegradation and bioresorption of calcium phosphate ceramics", Clin Mater, 14, pp.65~88 (1993)
- Chang MC, "Preparation of hydroxyapatitegelatin nanocomposites", Biomaterials, 24, pp.2853~2862 (2003)
- Brown WE, "A new calcium phosphate water setting cement. In Cements research progress", Am. Ceram. Soc, pp.352~379 (1986)
- Shindo ML, "Facial skeletal augmentation using hydroxyapatite cement", Arch Otolaryngol Head Neck Surg, 119, pp.185~190 (1993)
- Friedman CD, "BoneSourceTM hydroxyapatite cement: a novel biomaterial for craniofacial skeletal tissue engineering and reconstruction", J Biomed Mater Res, 43B, pp.428~432 (1998)
- Sugawara A, "Histopathological reaction of a calcium phosphate cement root canal filler", J Hard Tissue Biol, 4, pp.1~7 (1995)
- Cherng AM, "In vitro evaluation of a calcium phosphate cement root canal filler", sealer, 2001, pp.27, 613~615
- Dickens-Venz SH, "Physical and chemical properties of resin-reinforced calcium phosphate cements", Dent Mater, 10, pp.100~106 (1994)
- Dickens SH, "Mechanical properties and biochemical activity of remineralizing resin-based a-PO4 cements", Dent Mater, 19, pp.558~566 (2003)
- Takagi S, "Properties of novel resorbable chitosan-calcium phosphate composites", Dent Mater, 19, pp.797~804 (2003)
- Xu HHK, "Synergistic reinforcement of in situ hardening calcium phosphate composite scaffold for bone tissue engineering", Biomaterials, 25, pp.1029~1037 (2004)
- Xu HHK, "Fast setting calcium phosphate-chitosan scaffold: mechanical properties and biocompatibility", Biomaterials, 26, pp.1337~1348 (2005)
- Di Martino A, "Chitosan: a versatile biopolymer for orthopaedic tissue-engineering", Biomaterials, 26, pp.5983~5990 (2005)
- Prabaharan M, "Review paper: chitosan derivatives as promising materials for controlled drug delivery", J Biomater Appl, 23, pp.5~36 (2008)
- Kim IY, "Chitosan and its derivatives for tissue engineering applications", BIOTECHNOLOGY ADVANCES, 26(1), pp.1~21 (2008), PERGAMON-ELSEVIER SCIENCE LTD
- Li X, "Collagen-based implants reinforced by chitin fibres in a goat shank bone defect model", Biomaterials, 27, pp.1917~1923 (2006)
- Padois K, "Effects of chitosan addition to self-setting bone cement", Biomed Mater Eng, 17, pp.309~320 (2007)
- Khashaba RM, "Comparative study of biocompatibility of newly developed calcium phosphate-based root canal sealers on fibroblasts derived from primary human gingiva and a mouse L929 cell line", Int Endod J, 42, pp.711~718 (2009)
- Dagang G, "Physicochemical properties of TTCP", DCPA system cement formed in physiological saline solution and its cytotoxicity, 2006, pp.77, 313~323
- Matsuya S, "Effect of mixing ratio and pH on the reaction between Ca4(PO4)2O and CaHPO4", J Mater Sci Mater Med, 11, pp.305~311 (2000)
- Sun-Kyung Lee, "Effect of Calcium Phosphate Cements on Growth and Odontoblastic Differentiation in Human Dental Pulp Cells", JOURNAL OF ENDOCRINOLOGY, 36(9), pp.1537~1542 (2010), SOC ENDOCRINOLOGY