Mineralization-inducing potentials of calcium silicate-based pulp capping materials in human dental pulp cells
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Background: To provide a long-term bacterial seal through the formation of reparative dentin bridge, calcium silicate-based pulp capping materials have been used at sites of pulpal exposure. The aim of this study was to evaluate the mineralization-inducing potentials of calcium silicate-based pulp capping materials (ProRoot MTA [PR], Biodentine [BD], and TheraCal LC [TC]) in human dental pulp cells (HDPCs). Methods: Specimens of test materials were placed in deionized water for various incubation times to measure the pH variation and the concentration of calcium released. The morphology of HDPCs cultured on the specimens was examined using a confocal laser scanning microscope (CLSM). Alizarin red S staining and alkaline phosphatase assays were used to evaluate mineralization-inducing potentials of the capping materials. Results: BD showed the highest calcium release in all test periods, followed by PR and TC. (p
Abstract
Background: To provide a long-term bacterial seal through the formation of reparative dentin bridge, calcium silicate-based pulp capping materials have been used at sites of pulpal exposure. The aim of this study was to evaluate the mineralization-inducing potentials of calcium silicate-based pulp capping materials (ProRoot MTA [PR], Biodentine [BD], and TheraCal LC [TC]) in human dental pulp cells (HDPCs). Methods: Specimens of test materials were placed in deionized water for various incubation times to measure the pH variation and the concentration of calcium released. The morphology of HDPCs cultured on the specimens was examined using a confocal laser scanning microscope (CLSM). Alizarin red S staining and alkaline phosphatase assays were used to evaluate mineralization-inducing potentials of the capping materials. Results: BD showed the highest calcium release in all test periods, followed by PR and TC. (p
참고문헌 (30)
- Smith AJ, "Seltzer and Bender’s dental pulp", pp.27~46 (2012), Quintessence Publishing
- Sangwan P, "Tertiary dentinogenesis with calcium hydroxide : a review of proposed mechanisms", Int Endod J, 46, pp.3~19 (2013)
- Okiji T, "Reparative dentinogenesis induced by mineral trioxide aggregate : a review from the biological and physicochemical points of view", Int J Dent, 2009, pp.464280~ (2009)
- Mente J, "Mineral trioxide aggregate or calcium hydroxide direct pulp capping : an analysis of the clinical treatment outcome", J Endod, 36, pp.806~813 (2010)
- Bogen G, "Direct pulp capping with mineral trioxide aggregate : an observational study", J Am Dent Assoc, 139, pp.305~315 (2008)
- Chang SW, "Effects of calcium silicate endodontic cements on biocompatibility and mineralization-inducing potentials in human dental pulp cells", J Endod, 40, pp.1194~1200 (2014)
- Gandolfi MG, "In vitro screening of the apatite-forming ability, biointeractivity and physical properties of a tricalcium silicate material for endodontics and restorative dentistry", Dent J, 1, pp.41~60 (2013)
- Suh B, "Polymerizable dental pulp healing, capping, and lining material and method for use. International patent A61K33", 42, 2008
- Hebling J, "Cytotoxicity of resin-based light-cured liners", Am J Dent, 22, pp.137~142 (2009)
- Camilleri J, "Hydration of Biodentine, Theracal LC, and a prototype tricalcium silicate-based dentin replacement material after pulp capping in entire tooth cultures", J Endod, 40, pp.1846~1854 (2014)
- Rashid F, "The effect of extracellular calcium ion on gene expression of bone-related proteins in human pulp cells", J Endod, 29, pp.104~107 (2003)
- Takita T, "Effect of mineral trioxide aggregate on proliferation of cultured human dental pulp cells", Int Endod J, 39, pp.415~422 (2006)
- Estrela C, "Calcium hydroxide : study based on scientific evidences", J Appl Oral Sci, 11, pp.269~282 (2003)
- Formosa LM, "The chemical properties of light-and chemical-curing composites with mineral trioxide aggregate filler", Dent Mater, 29, pp.e11~e19 (2013)
- Grech L, "Characterization of set Intermediate Restorative Material, Biodentine, Bioaggregate and a prototype calcium silicate cement for use as root-end filling materials", Int Endod J, 46, pp.632~641 (2013)
- Moghaddame-Jafari S, "Effect of ProRoot MTA on pulp cell apoptosis and proliferation in vitro", J Endod, 31, pp.387~391 (2005)
- Maeda H, "Mineral trioxide aggregate induces bone morphogenetic protein-2 expression and calcification in human periodontal ligament cells", J Endod, 36, pp.647~652 (2010)
- Camilleri J, "Characterization of hydration products of mineral trioxide aggregate", Int Endod J, 41, pp.408~417 (2008)
- Camilleri J, "Hydration characteristics of Biodentine and Theracal used as pulp capping materials", Dent Mater, 30, pp.709~715 (2014)
- Poggio C, "Cytocompatibility and antibacterial properties of capping materials", ScientificWorldJournal, 2014, pp.181945~ (2014)
- Corral Nunez CM, "Biodentine and mineral trioxide aggregate induce similar cellular responses in a fibroblast cell line", J Endod, 40, pp.406~411 (2014)
- Burridge K, "Focal adhesions, contractility, and signaling", Annu Rev Cell Dev Biol, 12, pp.463~518 (1996)
- Gronthos S, "Postnatal human dental pulp stem cells(DPSCs)in vitro and in vivo", Proc Natl Acad Sci U S A, 97, pp.13625~13630 (2000)
- Laurent P, "Biodentine(TM)induces TGF-β1 release from human pulp cells and early dental pulp mineralization", Int Endod J, 45, pp.439~448 (2012)
- Nowicka A, "Tomographic evaluation of reparative dentin formation after direct pulp capping with Ca(OH)2, MTA, Biodentine, and dentin bonding system in human teeth", J Endod, 41, pp.1234~1240 (2015)
- Tziafa C, "Dentinogenic responses after direct pulp capping of miniature swine teeth with Biodentine", J Endod, 40, pp.1967~1971 (2014)
- Chung H, "Evaluation of physical and biologic properties of the mixture of mineral trioxide aggregate and 4-META", MMA-TBB resin, 2011, pp.112, e6~e11
- Hanks CT, "Cytotoxic effects of resin components on cultured mammalian fibroblasts", J Dent Res, 70, pp.1450~1455 (1991)
- Michelsen VB, "Identification of organic eluates from four polymer-based dental filling materials", Eur J Oral Sci, 111, pp.263~271 (2003)
- Ji Hyun Kwon, "Inhibition of odontogenic differentiation of human dental pulp cells by dental resin monomers", 생체재료학회지, 19(2), pp.99~105 (2015), 한국생체재료학회[DOI]