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Effects of Chitosan on the Differentiation of MDPC-23 Cells

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초록

The effects of chitosan upon the experimentally induced differentiation of MDPC-23 cells, derived from mouse dental papilla cells, were investigated by RT-PCR, observations of cell morphology and Alizaline red-S staining. Chitosan was found to significantly increase and accelerate the expression of ALP mRNA but decrease the ColI transcript levels, as compared with the control, in a time-dependent manner during the differentiation of MDPC-23 cells. Chitosan also significantly downregulated ON mRNA expression and accelerated mineralization in differentiating MDPC-23 cells. These results suggest that chitosan facilitates odontoblast differentiation and mineralization and may have potential clinical applications as a dentin regeneration material.

Abstract

The effects of chitosan upon the experimentally induced differentiation of MDPC-23 cells, derived from mouse dental papilla cells, were investigated by RT-PCR, observations of cell morphology and Alizaline red-S staining. Chitosan was found to significantly increase and accelerate the expression of ALP mRNA but decrease the ColI transcript levels, as compared with the control, in a time-dependent manner during the differentiation of MDPC-23 cells. Chitosan also significantly downregulated ON mRNA expression and accelerated mineralization in differentiating MDPC-23 cells. These results suggest that chitosan facilitates odontoblast differentiation and mineralization and may have potential clinical applications as a dentin regeneration material.

참고문헌 (39)

  1. About I, "Human dentin production in vitro", Exp Cell Res, 258, pp.33~41 (2000)
  2. Begue-Kirn C, "Comparative analysis of TGF beta s, BMPs, IGF1,msxs, fibronectin, osteonectin and bone sialoprotein gene expression during normal and in vitro-induced odontoblast differentiation", Int J Dev Biol, 38, pp.405~420 (1994)
  3. Bosshardt DD, "Hertwig's epithelial root sheath,enamel matrix proteins, and initiation of cementogenesis in porcine teeth", J Clin Periodontol, 31, pp.184~192 (2004)
  4. Chien HH, "Expression of TGF-beta isoforms and their receptors during mineralized nodule formation by rat periodontal ligament cells in vitro", J Periodontal Res, 34, pp.301~309 (1999)
  5. Dodane V, "Effect of chitosan on epithelial permeability and structure", Int J Pharm, 182, pp.21~32 (1999)
  6. D'Souza RN, "Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo", J Bone Miner Res, 12, pp.2040~2049 (1997)
  7. Finisie MR, "Synthesis of calcium-phosphate and chitosan bioceramics for bone regeneration", An Acad Brad Cienc, 73, pp.525~332 (2001)
  8. Foster BL, "Regulation of cementoblast gene expression by inorganic phosphate in vitro", Calcif Tissue Int, 78, pp.103~112 (2006)
  9. Gallaher CM, "Gallaher DD. Cholesterol reduction by glucomannan and chitosan is mediated by changes in cholesterol absorption and bile acid and fat excretion in rats", J Nutr, 130, pp.2753~2759 (2000)
  10. Gronthos S, "Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo", Proc Natl Acad Sci USA, 97, pp.13625~13630 (2000)
  11. Hanks CT, "Dentin-specific proteins in MDPC-23 cell line", Eur J Oral Sci, 106(Suppl 1), pp.260~266 (1998)
  12. Hanks CT, "Wataha JC,Ritchie HH, Butler WT.(b) Cloned 3T6 cell line from CD-1 mouse fetal molar dental papillae", Connect Tissue Res, 37, pp.233~249 (1998)
  13. Hirano S, "Chitin biotechnology applications", Biotechnol Annu Rev, 2, pp.237~258 (1996)
  14. In-Nam Hwang, "Mechanism underlying NO-induced apoptosis in human gingival fibroblasts", International Journal of Oral Biology, 34(1), pp.7~14 (2009), 대한구강생물학회
  15. Illum L, "Chitosan and its use as a pharmaceutical excipient", Pharm Res, 15, pp.1326~1331 (1998)
  16. 장민자, "유전자 전달체로서의 응용을 위한 키토산-DNA 복합체의 특성", Journal of Chitin and Chitosan, 11(3), pp.175~179 (2006), 한국키틴키토산학회
  17. Ji Hae Jun, "Bone Morphogenetic Protein 2-induced MAPKs Activation Is Independent of the Smad1", 5 Activation, 2009, pp.34(2), 115~121[DOI]
  18. Lee YM, "Tissue engineered bone formation using chitosan", tricalcium phosphate sponges, 2000, pp.71, 410~417
  19. Mina M, "The induction of odontogenesis in nondental mesenchyme combined with early murine mandibular arch epithelium", Arch Oral Biol, 32, pp.123~127 (1987)
  20. 남정표, "저분자량 수용성 N,O-carboxymethyl 키토산 제조 및 NMR를 이용한 구조 분석", Journal of Chitin and Chitosan, 12(3), pp.151~155 (2007), 한국키틴키토산학회
  21. Nanci A, "Ten Cate's oral histology: development, structure and function. 6th ed", pp.78~103 (2003), Mosby
  22. Narayanan K, "Differentiation of embryonic mesenchymal cells to odontoblast-like cells by overexpression of dentin matrix protein 1", Proc Natl Acad Sci USA, 98, pp.4516~4521 (2001)
  23. Ormrod DJ, "Dietary chitosan inhibits hypercholesterolaemia and atherogenesis in the apolipoprotein E-deficient mouse model of atherosclerosis", Atherosclerosis, 138, pp.329~334 (1998)
  24. Pae HO, "Induction of granulocytic differentiation in acute promyeloocytic leukemia cells (HL-60) by water-soluble chitosan oligomer", Leuk Res, 25, pp.339~346 (2001)
  25. Papagerakis P, "Investigation of osteocalcin,osteonectin, and dentin sialophosphoprotein in developing human teeth", Bone, 30, pp.377~385 (2002)
  26. Peluso G, "Chitosan-mediated stimulation of macrophage function", Biomaterials, 15, pp.1215~1220 (1994)
  27. Porporatto C, "Chitosan induces different L-arginine metabolic pathways in resting and inflammatory macrophages", Biochem Biophys Res Commun, 304, pp.266~272 (2003)
  28. Ranaldi G, "The effect of chitosan and other polycations on tight junction permeability in the human intestinal Caco-2 cell line", J Nutr Biochem, 13, pp.157~167 (2002)
  29. Ritchie HH, "Six decades of dentinogenesis research. Historical and prospective views on phosphophoryn and dentin sialoprotein", Eur J Oral Sci, 106(Suppl 1), pp.211~220 (1998)
  30. Schipper NGM, "Chitosans as absorption enhancers for poorly absorbable drugs : Mechanism of absorption enhancement", Pharm Res, 14, pp.923~929 (1997)
  31. Schipper NGM, "Chitosans as absorption enhancers for poorly absorbable drugs : Influence of molecular weight and degree of acetylation on drug transport across human intestinal epithelial (Caco-2) cells", Pharm Res, 13, pp.1686~1692 (1996)
  32. Schipper NGM, "Chitosans as absorption enhancers of poorly absorbable drugs: Influence of mucus on absorption enhancement", Eur J Pharm Sci, 8, pp.335~343 (1999)
  33. Shibuya N, "Retinoic acid is a potential negative regulator for differentiation of human periodontal ligament cells", J Periodontal Res, 40, pp.432~440 (2005)
  34. Sreenath T, "Dentin sialophosphoprotein knockout mouse teeth display widened predentin zone and develop defective dentin mineralization similar to human dentinogenesis imperfecta type III", J Biol Chem, 278, pp.24874~24880 (2003)
  35. Sun ZL, "Expression of dentin sialoprotein (DSP) and other molecular determinants by a new cell line from dental papillae, MDPC-23", Connect Tissue Res, 37, pp.251~261 (1998)
  36. Telles PD, "Lipoteichoic acid up-regulates VEGF expression in macrophages and pulp cells", J Dent Res, 82, pp.466~470 (2003)
  37. Tokoro A, "Growth-inhibitory effect of hexa-N-acetylchitohexanose and chitohexanose against Meth-A solid tumor", Chem Pharm Bull, 86, pp.784~790 (1988)
  38. Tziafas D, "Inductive influences of demineralized dentin and bone matrix on pulp cells: an approach of secondary dentinogenesis", J Dent Res, 69, pp.75~81 (1990)
  39. Tziafas D, "Role of exogenous TGF-beta in induction of reparative dentinogenesis in vivo", Eur J Oral Sci, 106(Suppl 1), pp.192~196 (1998)

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