무혈성 괴사에 이환된 대퇴골두 연골세포의 세포외 기질 mRNA 발현
The Expression of Extracellular Matrix mRNA in the Chondrocytes of the Femoral Head Affected by Avascular Necrosis
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목적: 대퇴골두 무혈성 괴사의 관절 연골세포에서 제 2형 교원질, aggrecan, vascular endothelial growth factor A (VEGF-A), pigmented epithelium-derived factor (PEDF) mRNAs의 발현 정도를 분석하였다. 대상 및 방법: 대퇴골두 무혈성 괴사로 인공 고관절 전치환술을 시행한 환자의 대퇴골두 관절연골에서 분리 배양한 연골세포를 삼차원 배양하여 제 2형 교원질, aggrecan, VEGF-A mRNA, PEDF mRNA 발현을 실시간 중합효소 연쇄반응법(Real-time PCR)으로 측정하였다. 결과: 대퇴골두 무혈성 괴사군의 관절 연골세포에서 제2 형 교원질과 aggrecan mRNA의 발현은 감소하였다(p<0.05). VEGFA mRNA와 PEDF mRNA의 발현은 정상 연골에 비해 차이가 없었다(p>0.05). 결론: 무혈성 괴사에 이환된 대퇴골두 연골세포의 연골 기질 형성 능력이 떨어져 있었다.
Abstract
Purpose: The aim of this study was to evaluate the expression of type Ⅱ collagen, aggrecan, VEGF-A and PEDF mRNAs in the human chondrocytes derived from the articular cartilage of the femoral heads with avacular necrosis (AVN). Materials and Methods: We cultured human chondrocytes that were primarily derived from the articular cartilage of femoral heads with AVN. We evaluated the mRNA expression of type Ⅱ collagen, aggrecan, VEGF-A and PEDF. Results: The chondrocytes of the AVN group showed decreased expressions of type Ⅱ collagen mRNA and aggrecan mRNA (p<0.05). The expression of VEGF-A mRNA and PEDF mRNA in the AVN group was the same as that of the normal group (p>0.05). Conclusion: The cartilage matrix’s formation ability was found to be decreased in the chondrocytes of the femoral heads affected by AVN.
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