대퇴골두 무혈성 괴사증에서 지질대사 및 PeroxisomeProliferator-Activated Receptor-γ2 유전자 발현의 의의
Significance of Lipid Profiles and Peroxisome Proliferator-Activated Receptor-γ2 Expression inOsteonecrosis of Femoral Head
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목적: 지질대사의 변화 및 PPARγ2 mRNA 의 발현이 대퇴골두 무혈성 괴사에 미치는 영향에 대하여 조사하고자 한다 대상 및 방법: 130명의 대퇴골두 무혈성 괴사 환자 및 30명의 대조군에서 lipid profile 을 조사하였다. 17명의 고관절 치환술 환자의 수술 시 얻은 골수세포를 이용하여 RT-PCRs 방법으로 PPARγ2 mRNA의 발현을 보았다. 결과: 유리 지방산은 스테로이드군이 666.5±163.4 mg/dl, 알코올군이 676.9±264.7 mg/dl로서, 468.5±194.1 mg/dl인 특 발성군과 453.5±169.3 mg/dl인 대조군에 비하여 유의하게 높았다. 중성지방은 스테로이드군이 183.6±58.4 mg/dl, 알코올 군이 223.7±70.9 mg/dl로서, 130.9±63.1 mg/dl인 특발성군과 93.1±79.0 mg/dl인 대조군에 비하여 유의하게 높았다. PPARγ2 mRNA 의 발현은 대퇴골두 무혈성 괴사 환자군이 다른 군에 비하여 유의하게 증가되어 있었다. 결론: 대퇴골두 무혈성 괴사에서 고지혈증은 위험인자로서 인식되며 의미있게 증가된PPARγ와의 관계는 향 후 지속적인 연구 가 필요할 것으로 사료된다.
Abstract
Purpose: To analyze the possible involvement of a change in the lipid metabolism and PPARγ2 level in the occurrence of ON of the femoral head. Material and Methods: The lipid profiles (Cholesterol, Triglyceride, LDL, HDL and Free fatty acid) of 130 patients with ON of the femoral head and 30 control persons were evaluated. The level of PPARγ2 mRNA expression was examined by performing, RT-PCR using the bone marrow stromal cells obtained from 17 patients during THA. Results: Among the 130 patients, the free fatty acid level in the alcohol (676.9±264.7 mg/dl,) and steroid (666.0 ±163.4 mg/dl) induced ON groups was higher than the control (453.5±169.3 mg/dl) and idiopathic (468.5±194.1 mg/dl) groups. The triglyceride level in the alcohol (223.7±70.9 mg/dl) and steroid (183.6?58.4 mg/dl) induced groups was higher than the control (93.1±79.0 mg/dl) and idiopathic groups (130.9±63.1 mg/dl). The level of PPARγ2 mRNA expression in the ON patients was significantly higher than the other groups (P <0.05). Conclusion: These results are significant and suggest hyperlipidemia as a risk factor for ON of the femoral head. Higher expression of PPARγ2 mRNA was found in ON. However, functional studies of PPAR?2 mRNA in vivo would be needed to reveal the pathogenesis of ON of the femoral head.
참고문헌 (21)
- Auwerx J, "Transcription, adipocyte differentiation, and obesity", 74, pp.347~352 (1996)
- Braissant O, "Differential expression of peroxisome proliferator-activated receptors (PPARs): tissue distribution of PPAR-alpha, -beta, and -gamma in the adult rat", 137(l), pp.354~366 (1996)
- Diascro DD Jr, "High fatty acid content in rabbit serum is responsible for the differentiation of osteoblasts into adipocyte-like cells", 13, pp.96~106 (1998)
- "Understanding the mechanism of LCAT reaction may help to explain the high predictive value of LDL", HDL cholesterol ratio, 1998, pp.47, 387~397
- "Molecular cloning, expression and characterization of human peroxisome proliferator activated receptors gamma 1 and gamma 2", 224, pp.431~437 (1996)
- "PPAR gamma:an essential role in metabolic control", 11, pp.64~69 (2001)
- "Association of antithrombotic factor deficiencies and hypofibrinolysis with Legg-Perthes disease", 78(1), pp.3~13 (1996)
- "major cause of osteonecrosis", hypofibri(lysis), pp.156 492~166 (1994)
- "Activation of a member of thesteroid hormone receptor superfamily by peroxisomeproliferators", pp.645~650 (1990)
- "Steroid-inducedaccumulation of lipid in the osteocytes of the rabbitfemoral head", pp.755~763 (1985)
- "A prostaglandin J2 metabolite binds peroxisome proliferator-activated receptor gamma and promotes adipocyte differentiation", 83, pp.813~819 (1995)
- "Nontraumatic necrosis of bone", pp.1473~1479 (1992)
- "Influence of alcohol intake andoccupational ststus on idiopathic osteonecrosis of thefemoral head", pp.115~123 (1988)
- "Non-traumatic avascular necrosis of the femoral head", 77A, pp.459~474 (1995)
- "Hypercholesterolemia: an associatiation with osteonecrosis of the femoral head", 26, pp.609~612 (1997)
- "Human trabecular bone cells are able to express both osteoblastic and adipocytic phenotype: implications for osteopenic disorders", 13, pp.371~382 (1998)
- "risk factors ofavascular necrosis of the femoral head in patients withSLE under high-dose corticosteroid therapy", pp.89~97 (1992)
- "Leptin acts on human marrow stromal cells to enhance differentiation to osteoblasts and to inhibit differentiation to adipocytes", 140(l), pp.1630~1638 (1999)
- "Stimulation ofadipogenesis in fibroblasts by PPAR gamma 2", tonto(z p), pp.1147~1156 (1994)
- "Steroid-induced femoral head pressure changes and theirresponse to lipid-clearing agents", pp.298~302 (1983)
- "Alcohol-induced adipogenesis in bone and marrow: a possible mechanism for osteonecrosis", 410, pp.213~224 (2003)