Effect of Zoledronate on the Expression of Vascular Endothelial Growth Factor-A by Articular Chondrocytes and Synovial Cells: An in Vitro Study
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Background: The aim of this in vitro study was to determine the effect of zoledronate,which is frequently used to treat osteoporosis, on osteoarthritis by analyzing zoledro-nate-induced expression of vascular endothelial growth factor-A (VEGF-A) in chondro-cytes and synovial cells. Methods: After chondrocytes and synovial cells were separatedand cultured, zoledronate was added, and VEGF-A and pigment epithelium-derived fac-tor (PEDF) expression were quantified by real-time polymerase chain reaction and West-ern blotting. Results: There was no significant difference in the expression of VEGF-AmRNA in chondrocytes between the zoledronate group and the control group on the8th day of culture. The expression of both VEGF-A and PEDF mRNA in synovial cells wassignificantly decreased in the zoledronate group (P<0.05). Conclusions: Zoledronatedecreases the expression of VEGF-A in synovial cells and may affect the developmentand progression of osteoarthritis.
Abstract
Background: The aim of this in vitro study was to determine the effect of zoledronate,which is frequently used to treat osteoporosis, on osteoarthritis by analyzing zoledro-nate-induced expression of vascular endothelial growth factor-A (VEGF-A) in chondro-cytes and synovial cells. Methods: After chondrocytes and synovial cells were separatedand cultured, zoledronate was added, and VEGF-A and pigment epithelium-derived fac-tor (PEDF) expression were quantified by real-time polymerase chain reaction and West-ern blotting. Results: There was no significant difference in the expression of VEGF-AmRNA in chondrocytes between the zoledronate group and the control group on the8th day of culture. The expression of both VEGF-A and PEDF mRNA in synovial cells wassignificantly decreased in the zoledronate group (P<0.05). Conclusions: Zoledronatedecreases the expression of VEGF-A in synovial cells and may affect the developmentand progression of osteoarthritis.
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