본문 바로가기
KERD
논문KCI 등재

Differential Proteomic Analysis of Chinese fir Clone Leaf Response to Salicylic Acid

Mei Yang|Sizu Lin|Guangqiu Cao
산림과학연구소 (The institute of forest science, kangwon national university)|2010.08|Journal of forest and environmental science|제26권 제2호|pp. 83-94

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

Chinese firSalicylic acid stressTwo-dimensional gel electrophoresisMALDI-TOF-TOF/MS

초록

Chinese fir (Latin name: Cunninghaimia lanceolata) is one of the major commercial coniferous trees. Most of Chinese fir forests are managed in successive rotation sites, which lead productivity to decline. Autotoxicity is the important reason for soil degradation of Chinese fir plantation, especially, phenolic acids are considered as the major allelopathic toxins which induce autotoxicity in Chinese fir rotation stands. We performed here proteomic approach to investigate the response of proteins in Chinese fir leaves to salicylic acid. The tube plantlets of Chinese fir clone were treated with 120 mg/L salicylic acid for 1, 3 and 5th day. 2-DE, coupled with MALDI-TOF-TOF/ MS, was used to separate and identify the responsive proteins. We found 12, 7, and 12 candidate protein spots that were up- or down-regulated by at least 2.5 fold after 1, 3, and 5th day of the stress, respectively. Of these protein spots, 16 spots were identified successfully. According to the putative physiological functions, these proteins were categorized into five classes (1) the proteins involved in protein stability and folding, including 26S proteome, Grp78, Hsp70, Hsp90 and PPIase; (2) the protein involved in photosynthesis and respiration, including OEC 33 kDa subunit, GAPDH; (3) the protein related to cell endurance to acid, F-ATPase; (4) the protein related to cytoskeleton, tubulin; (5) the protein related to protein translation: prolyl-tRNA synthetase. These results give new insights into autotoxic substance stress response in Chinese fir leaves and provide preliminary footprints for further studies on the molecular signal mechanisms induced by the stress.

Abstract

Chinese fir (Latin name: Cunninghaimia lanceolata) is one of the major commercial coniferous trees. Most of Chinese fir forests are managed in successive rotation sites, which lead productivity to decline. Autotoxicity is the important reason for soil degradation of Chinese fir plantation, especially, phenolic acids are considered as the major allelopathic toxins which induce autotoxicity in Chinese fir rotation stands. We performed here proteomic approach to investigate the response of proteins in Chinese fir leaves to salicylic acid. The tube plantlets of Chinese fir clone were treated with 120 mg/L salicylic acid for 1, 3 and 5th day. 2-DE, coupled with MALDI-TOF-TOF/ MS, was used to separate and identify the responsive proteins. We found 12, 7, and 12 candidate protein spots that were up- or down-regulated by at least 2.5 fold after 1, 3, and 5th day of the stress, respectively. Of these protein spots, 16 spots were identified successfully. According to the putative physiological functions, these proteins were categorized into five classes (1) the proteins involved in protein stability and folding, including 26S proteome, Grp78, Hsp70, Hsp90 and PPIase; (2) the protein involved in photosynthesis and respiration, including OEC 33 kDa subunit, GAPDH; (3) the protein related to cell endurance to acid, F-ATPase; (4) the protein related to cytoskeleton, tubulin; (5) the protein related to protein translation: prolyl-tRNA synthetase. These results give new insights into autotoxic substance stress response in Chinese fir leaves and provide preliminary footprints for further studies on the molecular signal mechanisms induced by the stress.

참고문헌 (64)

  1. Ahsan, N., "Excess copper induced physiological and proteomic changes in germinating rice seeds", Chemosphere, 67, pp.1182~1193 (2007)
  2. Beckmann, R.P., "Interaction of Hsp 70 with newly synthesized proteins: implications for protein folding and assembly", Science, 248(4957), pp.850~854 (1990)
  3. Berry, M.D., "Glyceraldehyde-3-phosphate dehydrogenase and apoptosis", J Neurosci Res, 60, pp.150~154 (2000)
  4. Bongani, K. N., "Identification of Arabidopsis salt and osmotic stress responsive proteins using two-dimensional difference gel electrophoresis and mass spectrometry", Proteomics, 5, pp.4185~4196 (2005)
  5. Boston, R.S., "Molecular chaperones and protein folding in plants", Plant molecular biology, 32(1-2), pp.191~222 (1996)
  6. Bradford, M. M, "A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding", Anal Biochem, 72, pp.248~254 (1976)
  7. Cao, G.Q., "Effects of the ferulic acid and cinnamic acid on the germination of Chinese fir seeds", Journal of Plant Resources and environment, 10(2), pp.63~64 (2001)
  8. Chen, L.C., "Effect of phenolics on 15N nutrient absorption and distribution of Cunninghahia lanceolata", Acta Phytoecologica Sinic, 26(5), pp.525~532 (2002)
  9. Chen, L.C., "Effect of vanillin and P-hydroxybenzoic acid on physiological characteristics of Chinese fir seedlings", Chinese Journal of Applied Ecology, 13(10), pp.1291~1294 (2002)
  10. Chen, L.C., "Preliminary study of allelopathy of root exudates of Chinese fir", Acta Ecologica Sinica, 23(2), pp.393~398 (2003)
  11. Cho, E. K., "Over-expression of tobacco NtHSP 70-1 contributes to drought-stress tolerance in plants", Plant cell reports, 25(4), pp.349~358 (2006)
  12. Chu, B, "Alteration of β-tubulin gene expression during low-temperature exposure in leaves of Arabidopsis thaliana", Plant Physiol, 103, pp.371~377 (1993)
  13. Cotler, P.D, "Surviving the acid test responses of gram-positive bacteria to low pH", Micrnbiol MoL BioL Rez, 67(3), pp.429~453 (2003)
  14. Damerval, C., "Technical improvements in two-dimensional electrophoresis increase the level of benetic variation detected in wheat-seeding proteins", Electrophoresis, 7, pp.52~54 (1986)
  15. Du, L., "Allelopathic effect of extractor of Chinese-fir rhizosphere soil on germination of Chinese-fir seed", Acta Botanica Boreali- Occidentalia Sinica, 23(2), pp.323~327 (2003)
  16. Duy, N. V., "The proteome and transcriptome analysis of Bacillus subtilis in response to salicylic acid", Proteomics, 7, pp.698~710 (2007)
  17. Fruman, D. A., "Immunophilins in protein folding and immunosuppression", FASEB J, 8, pp.391~ (1994)
  18. Fu, Y., "Glucose regulated proteins in cancer progression, drug resistance and immunotherapy", Cancer Biol Ther, 5(7), pp.741~744 (2006)
  19. Gao, Z.X., "Study on phenol degradation in Ralstonia metallidurans CH-34", Microbiology, 32(1), pp.44~ (2005)
  20. Goldberg, A.L, "Protein degradation and protection against misfolded or damaged proteins", Nature, 426(6968), pp.895~899 (2003)
  21. Guy, C.L., "The organization and evolution of the spinach stress 70 molecular chaperone gene family", Plant cell, 10(4), pp.539~556 (1998)
  22. Hati, S., "Pretransfer editing by class II prolyl-tRNA synthetase: role of aminoacylation active site in “selective release” of noncognate amino acids", J Biol Chem, 281, pp.27862~27872 (2006)
  23. He, H.Q., "Analyzing functional proteomics of weed-suppressing effect of allelopathic rice", Journal of genetics and genomics, 31(9), pp.1018~1023 (2004)
  24. Henmi, T., "Release and reactive-oxygen-mediated damage of the oxygen-evolving complex subunits of PSⅡ during photoinhibition", Plant Cell Physiol, 45(2), pp.243~250 (2004)
  25. Hepler, P. K., "Behavior of microtubules in living plant cells", Plant Physiol, 112, pp.455~461 (1996)
  26. Herbik, A., "Iron and copper nutritiondependent changes in protein expression in a tomato wild type and the nicotianamine-free mutant chloronerva", Plant Physiol, 111(2), pp.533~540 (1996)
  27. Jian, L. C., "Study on the relationship between cold stability of microtubules and freezing tolerance of plant", Acta Botanica Sinica, 31, pp.737~747 (1989)
  28. Kuhnert, W. L., "Genetic and biochemical characterization of the F-ATPase operon from Streptococcus sanguis 10904", J Bacteriol, 185(5), pp.1525~1533 (2003)
  29. Li, J., "Stress induction of GRP78", BiP and its role in cancer, 2006, pp.6(1), 45~54
  30. Lin, S.K., "Proteomic analysis of the expression of proteins related to rice quality during caryopsis development and the effect of high temperature on expression", Proteomics, 5(8), pp.2140~2156 (2005)
  31. Lin, S.Z, "Changes of the chlorophyll content, membranous permeability and stoma of Chinese fir treated by aqueous extracts of several source plants", Chinese Journal of Ecological Agriculture, 11(3), pp.29~31 (2003)
  32. Lin, S.Z., "Autointoxication of Chinese fir", Chinese Journal of Applied Ecology, 10(6), pp.661~664 (1999)
  33. Lipman, R.S., "Amino acid activation of a dual-specificity tRNA synthetase is independent of tRNA", J Mol Bio, 316(3), pp.421~427 (2002)
  34. Lu,C.M., "Heat-induced multiple effects on PSII in wheat plants", J Plant Physiol, 156, pp.259~265 (2000)
  35. Lu, Y.K., "Carbonic anhydrase activity of the photosystem ii oec33 protein from pea", Plant Cell Physiol, 46, pp.1944~1953 (2005)
  36. Ma, X Q, "A study on selfpoisoning efects of Chinese fir plantation", Journal of Nanjing Forestry University, 24(1), pp.12~16 (2000)
  37. Ma, Y.Q, "Effect of vanillin on the growth of Chinese fir (Cunningghamia lanceolata) seedlings", Chinese Journal of Aapplied Ecology, 9(2), pp.128~132 (1998)
  38. Manjunath, S., "Molecular characterization and promoter analysis of the maize cytosolic glyceraldehydes-3-phosphate dehydrogenase gene family and its expression during anoxia", Plant Mol Bio1, 33, pp.97~ll2 (1997)
  39. Mayer, M. P., "Molecular chaperones: The busy life of Hsp90", Curr Biol, 9, pp.322~325 (1999)
  40. Mitsopoulos, C., "The prostate-derived sterile 20-like kinase (PSK) regulates microtubule organization and stability", J Biol Chem, 278, pp.18085~18091 (2003)
  41. Pi, R. B., "Glyceraldehyde-3-phosphate Dehydrogenase Neuronal Apoptosis", Chinese Journal of Neuroscience, 16(1), pp.73~75 (2000)
  42. Practorius-Ibba, M, "Association between Archaeal prolyl- and leucyl-tRNA synthetases enhances tRNA(Pro) aminoacylation", J Biol Chem, 280, pp.26099~27104 (2005)
  43. Rho S B, "Genetic dissection of protein interaction in multitRNA synthetase complex", Proc Natl Acad Sci USA, 96(8), pp.4488~4493 (1999)
  44. Rinfret, A., "The N-terminal cyclophilin-homologous domain of a 150-kilodalton tumor recognition molecule exhibits both peptidylprolyl cis-transisomerase and chaperone activities", Biochemistry, 33, pp.1668~ (1994)
  45. Sheng, J., "Enhanced acid resistance of oral streptococci at lethal pH values associated with acid-tolerant catabolism and with ATP synthase activity", FEMS Microbiol Lett, 262(1), pp.93~98 (2006)
  46. Shutova, T., "Analysis of pH-induced structural changes of the isolated extrinsic 33 kilodalton protein of photosystem II", Biochemistry, 36, pp.6350~6358 (1997)
  47. Stathopoulos, C., "Cysteinyl-tRNA synthetase is not essential for viability of the archaeon Methanococcus maripaludis", Proc Natl Acad Sci USA, 98, pp.14292~14297 (2001)
  48. Suzuki, T., "Role of F1F0-ATPase in the browth of Streptococcus mutants GS5", J Appl Microbiol, 88, pp.555~562 (2000)
  49. Svensson, B., "Structural studies of the manganese stabilizing subunit in photosystem II", Biophysical Journal, 86, pp.1807~1812 (2004)
  50. Wang,Q., "Over expression of endoplasmic reticulum molecular chaperone GRP94 and GRP78 in human lung cancer tissues and its significance", Cancer Detect Prev, 29(6), pp.544~551 (2005)
  51. Wang, S.L., "Effects of three kinds of allelochemicals on growth of Chinese fir seedlings", Chinese Journal of Applied and Environmental Biology, 8(6), pp.588~591 (2002)
  52. Wang, W., "Plant responses to drought, salinity and extreme temperatures, Towards genetic engineering for stress tolerance", Planta, 218, pp.1~14 (2003)
  53. Welin, J., "Effect of acid shock on protein expression by biofilm cells of Streptococcus mutans", TEMS Microbiol Lett, 227, pp.287~293 (2003)
  54. Yamane, Y., "Effects of high temperatures on the photosynthetic systems in spinach, oxygen-evolving activities, fluorescence characteristics and the denaturation process", Photosynthesis Research, 57, pp.51~59 (1998)
  55. Yamazaki, D., "Target proteins of the cytosolic thioredoxins in Arabidopsis thaliana", Plant Cell Physiol, 45(1), pp.18~27 (2004)
  56. Zhen, Y., "Comparative proteome analysis of differentially expressed proteins induced by Al toxicity in soybean", Physiologia Plantarum, 131, pp.542~554 (2007)
  57. Yang, M., "Optimization of two-dimensional polyacrylamide gel electrophoresis for proteome of Chinese fir leaves", Journal of Tropical and Subtropical Botany, 15(5), pp.438~442 (2007)
  58. Yang, M., "Allelopathic Effects of salicylic acid stress on membrane lipid peroxidation and osmosis-regulating substances in different Chinese fir clones", Acta Botanica Boreali-Occidentalia Sinica, 26(10), pp.2088~2093 (2006)
  59. Yang, M., "Variation characteristics of free amino acids in Chinese fir leaves under salicylic acid stress", Journal of Northeast Forestry University, 35(2), pp.40~41 (2007)
  60. Yang, Y., "Stress responses and metabolic regulation of glyceraldehydes-3-phosphate dehydrogenase genes in Arabidopsis", Plant Physiol, 101, pp.209~216 (1993)
  61. Yonehara, M., "Heat-induced chaperone activity of HSP90", Journal of Biological Chemistry, 271(5), pp.2641~2645 (1996)
  62. Zhang, J.W., "Influence of repeatedly planting soil on Cunninghaimia lanceolata sapling growth", Chinese Journal of Applied Ecology, 5(4), pp.241~244 (1994)
  63. Zhao, J.L., "Relationship between freezing tolerance of root-tip cells and cold stability of microtubules and tubulin in cucumber", Journal of Hebei University (Natural Science Edition), 26(2), pp.188~192 (2006)
  64. Zouni, A., "Crystal structure of photosystem II from synechococcus elongatus at 3.8Å resolution", Nature, 409(6821), pp.739~743 (2001)

관련 논문AI추천

Proteomic analysis of Korean ginseng (Panax ginseng C. A. Meyer) following exposure to salt stress강규영, 이경희, 임채오, 황정은, Dong-Won Bae, Dong Yun Hyun, Kyong-Hwan Bang, Nam Hee Yoo, Ok-Tae Kim, Sun Tae Kim, Young-Chang Kim한국식물생명공학회 · Journal of Plant Biotechnology2008 · 제35권 제3호Protein Profiles in Response to Salt Stress in Seedling of Salt Tolerant Rice Mutants강시용, 김동섭, 김상훈, 김진백, 송재영, 이경준, 이명철, 이영근, 하보근(사)한국방사선산업학회 · 방사선산업학회지2012 · 제6권 제2호초기 염류 스트레스 반응 인삼 잎 단백질체 분석김기홍, 김선태, 김소운, 김영창, 민철우, 방경환, 조익현, GUPTARAVI한국약용작물학회 · 한국약용작물학회지2014 · 제22권 제5호Comparative proteomic analysis of plant responses to sound waves in Arabidopsis권영상, 박수철, 배한홍, 신성철, 정우식, Dong-Won Bae, Jaeyul Cha1, Mi-Jeong Jeong, Sung Woo Jeong한국식물생명공학회 · Journal of Plant Biotechnology2012 · 제39권 제4호Identification of γ-Radiation-responsive Proteins in Arabidopsis thaliana김진홍, 박용대, 배형우, 이동기, 이성범, 이승식, 정광우, 정병엽, 조철오(사)한국방사선산업학회 · 방사선산업학회지2019 · 제13권 제2호Korean plant proteomics: pioneers in plant stress physiology강규영, 김상곤, 김선태, 김선형, 서상규, 심이성, Suhyeon Bea, Youngwoo Lee한국식물생명공학회 · Journal of Plant Biotechnology2011 · 제38권 제2호

이 간행물의 다른 논문 (8건)

Biodiversity in the Context of Management and Conservation of Forest Resource김지홍, 이종규, 김준순pp. 65-73Characterization of Biometry and Chemical and Morphological Properties of Fibers from Bagasse, Corn, Sunflower, Rice, and Rapeseed Residues in IranJafar Ebrahimpour Kasmani, Ahmad Samariha, Majid Kiaeipp. 75-82Floral Biology and Flowering Phenology of Jatropha CurcasRanbir S. Fougat, Tanmay K. Patel, Nitish Kumar, Amritpal S. Singh, Mukesh P. Patel, D. R. Delvadia, Diwaker R. Patel, Subhash Naraynanpp. 95-102Implications of Mangrove Wetland in Socio-environmental Sector: Experiences from Southeast Coast of Chittagong, BangladeshMd. Riaz Uddin, Md. Azim Ferdous, Md. Atiqul Islam Mondal, Md. Hadayet Ullahpp. 103-111Infestation of the Longhorned Beetles Species (Cerambycidae) on Acacia seyal Del var. seyal in the Gum Arabic Belt of SudanMaymoona Ahmed Eisa, Yahia Omar Adampp. 113-116
원문 보기