Protein Profiles in Response to Salt Stress in Seedling of Salt Tolerant Rice Mutants
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초록
Proteomic analysis was performed in order to identify proteomic changes by salt stressbetween the Japonica cv. Donganbyeo (WT) and two salt-tolerant (ST) mutant lines by using theSDS-PAGE and 2-DE. Two salt tolerant rice mutant lines, ST-87 and ST-301, were selected by invitro mutagenesis with gamma-ray. Three-week-old seedlings were treated with 171 mM NaCl for7 days. In the SDS-PAGE, three proteins with molecular weights of 27, 46 and 58 kDa were highlyincreased under salt treatment. Total proteins from shoots of both WT and ST-lines were separatedby two-dimensional gel electrophoresis. In 2-DE, 201, 226, 217 and 213 protein spots were detectedin the untreated-or treated-WT and untreated- or treated-ST-87, respectively. Of theses, 17 and 10protein spots were up- and down-regulated under salt stress in the WT, respectively. While, 16 and8 protein spots were up- and down-regulated under salt stress in the ST-87, respectively, comparedwith the untreated plants. High intensity or de novo synthesized proteins were analyzed by MALDITOF/MS analysis.
Abstract
Proteomic analysis was performed in order to identify proteomic changes by salt stressbetween the Japonica cv. Donganbyeo (WT) and two salt-tolerant (ST) mutant lines by using theSDS-PAGE and 2-DE. Two salt tolerant rice mutant lines, ST-87 and ST-301, were selected by invitro mutagenesis with gamma-ray. Three-week-old seedlings were treated with 171 mM NaCl for7 days. In the SDS-PAGE, three proteins with molecular weights of 27, 46 and 58 kDa were highlyincreased under salt treatment. Total proteins from shoots of both WT and ST-lines were separatedby two-dimensional gel electrophoresis. In 2-DE, 201, 226, 217 and 213 protein spots were detectedin the untreated-or treated-WT and untreated- or treated-ST-87, respectively. Of theses, 17 and 10protein spots were up- and down-regulated under salt stress in the WT, respectively. While, 16 and8 protein spots were up- and down-regulated under salt stress in the ST-87, respectively, comparedwith the untreated plants. High intensity or de novo synthesized proteins were analyzed by MALDITOF/MS analysis.
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