Identification of γ-Radiation-responsive Proteins in Arabidopsis thaliana
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Due to an increase in public concern about radiation after the Fukushima nuclearaccident, an array of studies have been executed to understand the biological effects of radiationusing model organisms such as Arabidopsis rather than humans. Although a large amount ofinformation about the biological effect of radiation has been accumulated, most of the studieswere performed at the transcriptional level. To systematically understand the biological effects ofradiation, here, we performed proteome analysis using A. thaliana in the two vegetative stages [early(10-day-old) and late (21-day-old) vegetative stages] and identified radiation-responsive proteins. This analysis revealed that the proteins involved in the oxidative stress, molecular chaperone,and general stress responses showed an abundant change post γ-radiation exposure. Notably, weidentified four proteins (acryl carrier protein 4, glycine cleavage system H protein 1, peptidylprolylcis-trans isomerase CYP20-3, and calreticulin-1) that showed protein expression changes inboth early and late vegetative stages after γ-radiation exposure. Furthermore, the Gene Ontology(GO) analysis revealed that proteins changed post γ-radiation exposure were mainly involved inprotein binding activities and responses to stress. Collectively, we reported that proteins involvedin oxidative stress and molecular chaperones showed an abundant change in response to radiationexposure in the vegetative stage of the A. thaliana.
Abstract
Due to an increase in public concern about radiation after the Fukushima nuclearaccident, an array of studies have been executed to understand the biological effects of radiationusing model organisms such as Arabidopsis rather than humans. Although a large amount ofinformation about the biological effect of radiation has been accumulated, most of the studieswere performed at the transcriptional level. To systematically understand the biological effects ofradiation, here, we performed proteome analysis using A. thaliana in the two vegetative stages [early(10-day-old) and late (21-day-old) vegetative stages] and identified radiation-responsive proteins. This analysis revealed that the proteins involved in the oxidative stress, molecular chaperone,and general stress responses showed an abundant change post γ-radiation exposure. Notably, weidentified four proteins (acryl carrier protein 4, glycine cleavage system H protein 1, peptidylprolylcis-trans isomerase CYP20-3, and calreticulin-1) that showed protein expression changes inboth early and late vegetative stages after γ-radiation exposure. Furthermore, the Gene Ontology(GO) analysis revealed that proteins changed post γ-radiation exposure were mainly involved inprotein binding activities and responses to stress. Collectively, we reported that proteins involvedin oxidative stress and molecular chaperones showed an abundant change in response to radiationexposure in the vegetative stage of the A. thaliana.
참고문헌 (39)
- Ali Khan H, "Protein disulfide isomerase a multifunctional protein with multiple physiological roles", Front Chem, 2, pp.70~ (2014)
- Angelini G, "A device to study the effect of space radiation on photosynthetic organisms", Phys Med, 17(Suppl 1), pp.267~268 (2001)
- Branen JK, "Expression of antisense acyl carrier protein-4 reduces lipid content in Arabidopsis leaf tissue", Plant Physiol, 132, pp.748~756 (2003)
- Cai H, "Overexpressed Glutamine synthetase gene modifies nitrogen metabolism and abiotic stress responses in rice", Plant Cell Rep, 28, pp.527~537 (2009)
- Caverzan A, "Plant responses to stresses : Role of ascorbate peroxidase in the antioxidant protection", Genet Mol Biol, 35, pp.1011~1019 (2012)
- Close DM, "DNA damage by the direct effect of ionizing radiation: products produced by two sequential one-electron oxidations", J. Phys. Chem. A, 117, pp.12608~12615 (2013)
- Dominguez-Solis JR, "A cyclophilin links redox and light signals to cysteine biosynthesis and stress responses in chloroplasts", Proc. Natl. Acad. Sci .U S A, 105, pp.16386~16391 (2008)
- Griffin TJ, "Complementary profiling of gene expression at the transcriptome and proteome levels in Saccharomyces cerevisiae", Mol. Cell. Proteomics, 1, pp.323~333 (2002)
- Guan M, "Cytosolic Glutamine Synthetase Gln1;2 Is the Main Isozyme Contributing to GS1 Activity and Can Be Up-Regulated to Relieve Ammonium Toxicity", Plant Physiol, 171, pp.1921~1933 (2016)
- Gutteridge BHaJ, "Free Radicals in Biology and Medicine" (1999), Oxford University Press
- Gygi SP, "Correlation between protein and mRNA abundance in yeast", Mol. Cell. Biol, 19, pp.1720~1730 (1999)
- Hallahan DE, "The role of intracellular calcium in the cellular response to ionizing radiation", Radiat. Res, 138, pp.392~400 (1994)
- Hanson AD, "One-Carbon Metabolism in Higher Plants", Annu. Rev. Plant. Physiol. Plant Mol. Biol, 52, pp.119~137 (2001)
- Hayashi G, "2D-DIGE-based proteome expression changes in leaves of rice seedlings exposed to low-level gamma radiation at Iitate village", Fukushima. Plant Signal. Behav, 10, pp.e1103406~ (2015)
- Hayashi G, "Unraveling low-level gamma radiation--responsive changes in expression of early and late genes in leaves of rice seedlings at Iitate Village, Fukushima", J. Hered, 105, pp.723~738 (2014)
- Hoshida H, "Enhanced tolerance to salt stress in transgenic rice that overexpresses chloroplast glutamine synthetase", Plant Mol. Biol, 43, pp.103~111 (2000)
- Hwang SG, "Transcriptome analysis of reproductive-stage Arabidopsis plants exposed gamma-ray irradiation at various doses", Int. J. Radiat. Biol, 92, pp.451~465 (2016)
- Jung KW, "Unraveling Fungal Radiation Resistance Regulatory Networks through the Genome-Wide Transcriptome and Genetic Analyses of Cryptococcus neoformans", MBio, 7, pp.e01483~ (2016)
- Kelemen O, "Function of alternative splicing", Gene, 514, pp.1~30 (2013)
- Kim DS, "Antioxidant response of Arabidopsis plants to gamma irradiation: Genome-wide expression profiling of the ROS scavenging and signal transduction pathways", J. Plant Physiol, 168, pp.1960~1971 (2011)
- Kim JH, "Integrated analysis of diverse transcriptomic data from Arabidopsis reveals genetic markers that reliably and reproducibly respond to ionizing radiation", Gene, 518, pp.273~279 (2013)
- Kim JH, "Transcriptomic profile of Arabidopsis rosette leaves during the reproductive stage after exposure to ionizing radiation", Radiat. Res, 168, pp.267~280 (2007)
- Kim JH, "Photosynthetic capacity of Arabidopsis plants at the reproductive stage tolerates gamma irradiation", J. Radiat. Res, 52, pp.441~449 (2011)
- Kim JH, "Loss of all three calreticulins, CRT1, CRT2 and CRT3, causes enhanced sensitivity to water stress in Arabidopsis", Plant Cell Rep, 32, pp.1843~1853 (2013)
- Kovalchuk I, "Transgenic plants are sensitive bioindicators of nuclear pollution caused by the Chernobyl accident", Nat. Biotechnol, 16, pp.1054~1059 (1998)
- Latijnhouwers M, "Arabidopsis stromal 70-kDa heat shock proteins are essential for chloroplast development", Planta, 232, pp.567~578 (2010)
- Liu SG, "Disrupted actin dynamics trigger an increment in the reactive oxygen species levels in the Arabidopsis root under salt stress", Plant Cell. Rep, 31, pp.1219~1226 (2012)
- Lothier J, "The cytosolic glutamine synthetase GLN1;2 plays a role in the control of plant growth and ammonium homeostasis in Arabidopsis rosettes when nitrate supply is not limiting", J. Exp. Bot, 62, pp.1375~1390 (2011)
- Motohashi K, "Chloroplast cyclophilin is a target protein of thioredoxin. Thiol modulation of the peptidyl-prolyl cistrans isomerase activity", J. Biol. Chem, 278, pp.31848~31852 (2003)
- Nagata T, "Radial expansion of root cells and elongation of root hairs of Arabidopsis thaliana induced by massive doses of gamma irradiation", Plant Cell Physiol, 45, pp.1557~1565 (2004)
- Nagata T, "Levels of active oxygen species are controlled by ascorbic acid and anthocyanin in Arabidopsis", J. Agric. Food Chem, 51, pp.2992~2999 (2003)
- Nagata T, "Microarray analysis of genes that respond to gamma-irradiation in Arabidopsis", J. Agric. Food Chem, 53, pp.1022~1030 (2005)
- Nylander M, "Stress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana", Plant Mol. Biol, 45, pp.263~279 (2001)
- Robertson KL, "Adaptation of the black yeast Wangiella dermatitidis to ionizing radiation: molecular and cellular mechanisms", PLoS ONE, 7, pp.e48674~ (2012)
- Romano PG, "The Arabidopsis cyclophilin gene family", Plant Physiol, 134, pp.1268~1282 (2004)
- Su PH, "Arabidopsis stromal 70-kD heat shock proteins are essential for plant development and important for thermotolerance of germinating seeds", Plant Physiol, 146, pp.1231~1241 (2008)
- Van de Walle J, "Arabidopsis plants exposed to gamma radiation in two successive generations show a different oxidative stress response", J. Environ. Radioact, 165, pp.270~279 (2016)
- Wilkinson B, "Protein disulfide isomerase", Biochim. Biophys. Acta, 1699, pp.35~44 (2004)
- Yi L, "Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays", PLoS ONE, 12, pp.e0182671~ (2017)