국내 및 미얀마에서 재배된 삼채뿌리의 항산화, α-Glucosidase 저해 및 Acetylcholinesterase 저해 활성
Evaluation of antioxidant, α-glucosidase inhibition and acetylcholinesterase inhibition activities of Allium hookeri root grown in Korea and Myanmar
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This study was conducted to compare the functionality (antioxidant, anti-diabetic, and anti-dementia activities) of the methanol extract of Allium hookeri root grown in Korea (KR) and Myanmar (MR). The total polyphenol and flavonoid contents of KR and MR were 5.27 and 4.80 mg GAE/g, and 0.35 and 0.24 mg QE/g, respectively. KR contained significantly higher levels of total polyphenols and total flavonoids than those of MR (p<0.05). The IC50 values of KR and MR were 6.53 and 5.31 mg/mL, respectively, for DPPH radical scavenging activity. However, KR had a significantly higher ABTS radical scavenging activity, Fe2+ chelating ability, and reducing power compared with those of MR (p<0.05). In the evaluation of anti-diabetic activity, KR showed significantly higher α-glucosidase inhibition activity than acarbose and MR at whole concentrations (p<0.05). KR and MR had acetylcholinesterase inhibition activities that of 51.44% and 44.33%, respectively, at a 50 mg/mL concentration. These results suggested that roots of A. hookeri, especially KR, could be useful in improving diabetic and dementia disorders due to their high antioxidant, anti-diabetic, and anti-dementia activities.
Abstract
This study was conducted to compare the functionality (antioxidant, anti-diabetic, and anti-dementia activities) of the methanol extract of Allium hookeri root grown in Korea (KR) and Myanmar (MR). The total polyphenol and flavonoid contents of KR and MR were 5.27 and 4.80 mg GAE/g, and 0.35 and 0.24 mg QE/g, respectively. KR contained significantly higher levels of total polyphenols and total flavonoids than those of MR (p<0.05). The IC50 values of KR and MR were 6.53 and 5.31 mg/mL, respectively, for DPPH radical scavenging activity. However, KR had a significantly higher ABTS radical scavenging activity, Fe2+ chelating ability, and reducing power compared with those of MR (p<0.05). In the evaluation of anti-diabetic activity, KR showed significantly higher α-glucosidase inhibition activity than acarbose and MR at whole concentrations (p<0.05). KR and MR had acetylcholinesterase inhibition activities that of 51.44% and 44.33%, respectively, at a 50 mg/mL concentration. These results suggested that roots of A. hookeri, especially KR, could be useful in improving diabetic and dementia disorders due to their high antioxidant, anti-diabetic, and anti-dementia activities.
참고문헌 (36)
- Ayam VS, "Allium hookeri, Thw. Enum. A lesser known terrestrial perennial herb used as food and its ethnobotanical relevance in manipur", Afr J Food Agric Nutr Dev, 11, pp.5389~5412 (2011)
- 김창현, "삼채 뿌리 메탄올 추출물이 LPS가 유도된 RAW264.7 세포에 대한 항염증 효과", 한국식품영양과학회지, 41(11), pp.1645~1648 (2012), 한국식품영양과학회
- 배기춘, "국내산 삼채 에탄올 추출물의 항염증 효과", 대한본초학회지, 27(6), pp.55~61 (2012), 대한본초학회
- 권은진, "양파 재배 중 식이유황 methylsulfonlymethane 처리가 양파의 품질 특성에 미치는 영향", 한국식품과학회지, 45(2), pp.213~220 (2013), 한국식품과학회[DOI]
- 박주영, "국내 및 미얀마에서 재배된 삼채뿌리의 영양성분 및 품질특성 비교", 한국식품과학회지, 46(5), pp.544~548 (2014), 한국식품과학회[DOI]
- Oh JY, "The growth characteristics of Allium hookeri as altitudes in Gyeongnam", Korean J Hor Sci Technol, 32(sup), pp.79~80 (2014)
- 원준연, "노지 및 시설재배 삼채 뿌리 및 잎의 이화학 성분, DPPH 라디칼 소거능 및 Nitric Oxide 생성 억제효과", 한국식품영양과학회지, 42(9), pp.1351~1356 (2013), 한국식품영양과학회
- 유보람, "삼채뿌리를 첨가한 김치의 품질 특성", 한국식품영양과학회지, 42(10), pp.1649~1655 (2013), 한국식품영양과학회
- Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", J Biol Chem, 12, pp.239~243 (1912)
- Moreno MIN, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", J Ethnopharmacol, 71, pp.109~114 (2000)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Bio Med, 26, pp.1231~1237 (1999)
- Dinis TC, "Action of phenolic derivatives(acetaminophen, salicylate, and 5-aminosalicylate)as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys", Arch Biochem Biophys, 315, pp.161~169 (1994)
- Mau JL, "Antioxidant properties of several specialty mushrooms", Food Res Int, 35, pp.519~526 (2002)
- Phan MAT, "Evaluation of α-glucosidase inhibition potential of some flavonoids from Epimedium brevicornum", LWT-Food Sci Technol, 53, pp.492~498 (2013)
- Ellman GL, "A new and rapid colorimetric determination of acetylcholinesterase activity", Biochem Pharmacol, 7, pp.88~95 (1961)
- 유미희, "생대추(풋대추) 과육 및 씨 추출물의 항산화 효과 및 항산화 성분", 한국식품과학회지, 38(1), pp.128~134 (2006), 한국식품과학회
- 최선영, "식용 및 약용자원의 에스트로젠 활성과 항산화능 평가", 한국식품과학회지, 37(4), pp.549~556 (2005), 한국식품과학회
- Wang M, "Antioxidative phenolic compounds from sage(Salvia officinalis)", J Agr Food Chem, 46, pp.4869~4873 (1998)
- 김주석, "붉은장목수수 추출물의 생리활성", 한국자원식물학회지, 26(1), pp.111~118 (2013), 한국자원식물학회[DOI]
- 권하나, "산겨릅나무 추출물의 항산화 및 간 기능 보호효과", 한국식품영양과학회지, 37(11), pp.1389~1394 (2008), 한국식품영양과학회
- Kang YH, "Studies on the physiological functionality of pine needle and mugwort extracts", Korean J Food Sci Technol, 27, pp.978~984 (1995)
- Labuza TP, "Kinetics of lipid oxidation in foods", Crit Rev Food Sci, 2, pp.355~405 (1971)
- Pasko P, "Anthocyanins, total polyphenols and antioxidant activity in amaranth and quinoa seeds and sprouts during their growth", Food Chem, 115, pp.994~998 (2009)
- Dlamini NR, "The effect of sorghum type and processing on the antioxidant properties of African sorghum-based foods", Food Chem, 105, pp.1412~1419 (2007)
- 최혜정, "사방오리나무 추출물의 α-amylase 및 α-glucosidase 저해활성", 생명과학회지, 18(7), pp.1005~1010 (2008), 한국생명과학회
- 정인경, "제2형 당뇨병 환자에서 Voglibose와 Acarbose의 비교임상연구", Diabetes and Metabolism Journal, 26(2), pp.134~145 (2002), 대한당뇨병학회
- Tsujimoto T, "Pneumatosis cystoides intestinalis following alpha-glucosidase inhibitor", World J Gastroenterol, 14, pp.6087~6092 (2008)
- 정현진, "약용식물의 추출방법에 따른 항산화 및 항당뇨 활성", 한국식품과학회지, 42(5), pp.571~577 (2010), 한국식품과학회
- Talesa VN, "Acetylcholinesterase in alzheimer's disease", Mech Ageing Dev, 122, pp.1961~1969 (2001)
- Houghton PJ, "Acetylcholinesterase inhibitors from plants and fungi", Nat Prod Rep, 23, pp.181~199 (2006)
- Muir JL, "Acetylcholine, aging, and alzheimer's disease", Pharmacol Biochem Behav, 56, pp.687~696 (1997)
- Berkov S, "Rapid TLC", GC-MS identification of acetylcholinesterase inhibitors in alkaloid extracts, 2008, pp.19, 411~419
- Mahinda Senevirathne, "Enzymatic Extracts from Edible Red Algae, Porphyra tenera, and Their Antioxidant, Anti-acetylcholinesterase, and Anti-inflammatory Activities", Food Science and Biotechnology, 19(6), pp.1551~1557 (2010), 한국식품과학회
- Jung MK, "Acetylcholinesterase inhibition by flavonoids from Agrimonia pilosa", Molecules, 12, pp.2130~2139 (2007)
- 정희록, "생마늘 추출 숙성물의 항산화 및 아세틸콜린에스터라아제 저해 효과", 농업생명과학연구, 45(4), pp.113~120 (2011), 농업생명과학연구원