재래종 대파(Allium fistulosum L.)의 생리 활성 평가
Evaluation of Physiological Activity of Traditional Welsh Onion (Allium fistulosum L.)
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목적: 본 연구는 재래종 대파(Allium fistulosum L.)의 부위별(꽃, 잎과 위경, 줄기, 뿌리) 열수추출물에 대한 생리 활성(항산화 활성및 효소 활성)을 분석하여 재래종 대파 연구의 기초자료를 제시하고자 하였다. 방법: 생리활성 분석을 위해 4 등분한 재래종 대파를부위별로 나누어 80℃에서 열수추출 하였다. 항산화 활성(total polyphenol 및 total flavonoid 함량, DPPH 및 ABTS radical 소거 활성)과 효소 활성(α-glucosidase, tyrosinase, elastase 저해 활성)을 측정하였다. 결과: 재래종 대파의 부위별 열수추출물의 항산화는 꽃(AFF)이 가장 높았다. 잎과 위경(AFLP), 줄기(AFS), 뿌리(AFR) 중에서는 뿌리의 total polyphenol 함량과 DPPH radical 소거활성이 높았으며, total flavonoid 함량과 ABTS radical 소거 활성에서는 줄기가 높았다. 효소 활성의 경우 α-glucosidase와 elastase 저해 활성은 뿌리에서, tyrosinase 저해 활성은 줄기가 가장 높았다. 결론: 재래종 대파인 구조파의 부위별 열수추출물의 생리 활성을 확인함으로써 대파 연구의 기초자료로 사용되길 바라며 향후 기능성 소재로의 활용가능성을 확인할 수 있었
Abstract
Purpose: This study was conducted to evaluate physiological activities, such as antioxidant activity and enzyme activity, of hot water extract of the four parts (flower, leaf and pseudostem, stem, and root) of traditional Welsh onion (Allium fistulosum L.) and then to provide basic data on traditional Welsh onion to support future research. Methods: Four parts of traditional Welsh onion plant were extracted with water heated at 80℃. Antioxidative and enzymatic activities, such as total polyphenol content, total flavonoid content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-bis-3-ethylbenzothiazoline–6-sulfonic acid (ABTS) radical scavenging activity, and the inhibitory activity on α-glucosidase, tyrosinase, and elastase enzymes, were measured. Results: Out of the hot water extract of the different parts, Allium fistulosum flower (AFF) extract had the highest content of antioxidants. In the Allium fistulosum leaf and pseudostem (AFLP), Allium fistulosum stem (AFS), and Allium fistulosum root (AFR) extracts, the total polyphenol content and DPPH radical scavenging activity of the root were high, and AFS had high total flavonoid content and ABTS radical scavenging activity. Regarding enzyme activity, α-glucosidase and elastase inhibitory activities were the highest in AFR, and tyrosinase inhibitory activity was the highest in AFS. Conclusion: We confirmed the physiological activity of the hot water extract of four parts of traditional Welsh onion. Our findings provide basic data for research into traditional Welsh onion, and can facilitate its future development as a functional material.
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