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삼채(Allium hookeri) 뿌리 추출물의 항산화 및 항염증 특성

Antioxidant and anti-inflammatory properties of extracts from Allium hookeri root

서혁준|강성국|김보섭|장성매|통타오|김총경|류야쥐안
한국식품저장유통학회 (The Korean Society of Food Preservation)|2015.12|Food Science and Preservation|제22권 제6호|pp. 867-877

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antioxidantAllium hookerianti-inflammatoryphenolicthiosulfinate
주제분류식품과학

초록

본 연구에서는 삼채 뿌리를 물, 에탄올, 메탄올로 추출했다. 추출방법에 따라 추출물의 항산화 및 항염증 효과는 다르게 확인됐다. 그 중에는 에탄올 추출물은 가장 좋은 효과를 확인하였으며 강한 환원력, DPPH 라디칼, superoxide 라디칼 및 아질산염 소거능이 나타났다 (IC50값은 각각 3.22, 4.26, 0.95, 3.22 mg/mL). 물 추출물과 메탄올 추출물은 좀 약한 효과를 나타났는데 좋은 hydroxyl 라디칼과 superoxide 라디칼 소거능을 볼 수 있다. RAW 264.7 세포를 통해서 측정한 결과에 따라 모두 추출물은 유익적인 염증 개성 효과를 나타냈다. 에탄올 추출물은 가장 높은 NO 생성 저해 활성을 나타내고 그 다음은 물 추출물과 메탄올 추출물을 순으로 나타냈다. Total phenolic, flavonoid하고 thiosulfinate의 함량은 다 에탄올 추출물은 제일 높은 농도를 가지고 있다 (각각 24.96 mg GAE/g extract, 4.27 mg RE/g extract, 14.2 μM/g extract). 연구결과에 따라 삼채 뿌리는 높은 total phenolic, total flavonoid하고 total thiosulfinate 함량을 함유하고 좋은 항산화 및 항염증 효과를 볼 수 있다.

Abstract

In this study, antioxidant and anti-inflammatory activities of water, methanol, and ethanol extracts obtained from Allium hookeri root were evaluated. The ethanol extract of A. hookeri was found to possess the strongest reducing power and also exhibited dominant effects on scavenging of nitrites, DPPH radicals, and superoxide radicals. The water extract showed more efficient DPPH and hydroxyl radical-scavenging activities than those of the methanol extract. Furthermore, the inhibitory activity against nitric oxide (NO) production in RAW 264.7 macrophages was evaluated to elucidate the anti-inflammatory properties of the extracts. Results indicated that all the extracts of A. hookeri exerted inhibitory activities against NO production, especially the ethanol extract (IC5029.13μg/mL). Total phenolic and total flavonoid contents were found to be abundant in the ethanol extract, with values of 24.96 mg gallic acid equivalent/g extract and 4.27 mg rutin equivalent/g extract, respectively. Total thiosulfinate content was determined for the first time and a high amount was present in the ethanol extract (14.2 μM/g extract). These results suggest that A. hookeri root has antioxidant and anti-inflammatory properties and could be used as a natural source for the development of pharmaceutical agents or functional foods.

참고문헌 (47)

  1. Kris-Etherton PM, "Bioactive compounds in nutrition and health-research methodologies for establishing biological function: the antioxidant and anti-inflammatory effects of flavonoids on atherosclerosis", Annu Rev Nutr, 24, pp.511~538 (2004)
  2. Halliwell B, "Oxygen toxicity, oxygen radicals, transition metals and disease", Biochem J, 219, pp.1~14 (1984)
  3. Tao Tong, "In vitro Antioxidant Potential and Inhibitory Effect of Seaweed on Enzymes Relevant for Hyperglycemia", Food Science and Biotechnology, 23(6), pp.2037~2044 (2014), 한국식품과학회[DOI]
  4. Corzo-Martı´nez M, "Biological properties of onions and garlic", Trends Food Sci Tech, 18, pp.609~625 (2007)
  5. Jensen GS, "In vitro and in vivo antioxidant and anti-inflammatory capacities of an antioxidant-rich fruit and berry juice blend. Results of apilot and randomized, double-blinded, placebo-controlled, crossoverstudy", J Agric Food Chem, 56, pp.8326~8333 (2008)
  6. Wong JM, "Regulation and function of inducible nitric oxide synthase during sepsis and acute inflammation", Adv Pharmacol, 6, pp.155~170 (1995)
  7. Knowles, RG, "Nitric oxide synthases in mammals", Biochem J, 298, pp.249~258 (1994)
  8. Yu T, "The pivotal role of TBK1 in inflammatory responses mediated by macrophages", Mediators Inflamm, pp.979~105 (2012)
  9. Shin IS, "Diallyl-disulfide, an organosulfur compound of garlic, attenuates airway inflammation via activation of the Nrf-2", HO-1 pathway and NF-kappa B suppression, 2013, pp.62, 506~513
  10. Park YC, "Activity of monomeric, dimeric, and trimeric flavonoids on NO production, TNF-α secretion, and NF-κB-dependent gene expression in RAW 264. 7 macrophages", FEBS Lett, 465, pp.93~97 (2000)
  11. Block E, "Allium Chemistry : HPLC analysis of thiosulfinates from onion, garlic, wild garlic (ramsoms), leek, scallion, shallot, elephant (great-headed) garlic, chive, and Chinese chive. Uniquely high allyl to methyl ratios in some garlic samples", J Agric Food Chem, 40, pp.2410~2430 (1992)
  12. Rose P, "Bioactive S-alk(en)yl cysteine sulfoxide metabolites in the genus Allium : the chemistry of potential therapeutic agents", Nat Prod Rep, 22, pp.351~368 (2006)
  13. 배기춘, "국내산 삼채 에탄올 추출물의 항염증 효과", 대한본초학회지, 27(6), pp.55~61 (2012), 대한본초학회
  14. 김창현, "삼채 뿌리 메탄올 추출물이 LPS가 유도된 RAW264.7 세포에 대한 항염증 효과", 한국식품영양과학회지, 41(11), pp.1645~1648 (2012), 한국식품영양과학회
  15. 이지영, "삼채 뿌리 추출물 및 분획물의 항균 및 항산화 활성", 아시안뷰티화장품학술지, 12(4), pp.533~538 (2014), 한국피부과학연구원
  16. Singleton VL, "Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents", Am J Enol Vitic, 16, pp.144~158 (1965)
  17. Jia ZS, "The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals", Food Chem, 64, pp.555~559 (1999)
  18. Han J, "A spectrophotometric method for quantitative determination of allicin and total garlic thiosulfinate", Anal Biochem, 225, pp.157~160 (1995)
  19. Yang XM, "Antioxidant and immunity activity of water extract and crude polysaccharide from Ficus carica L. fruit", Plant Foods Hum Nutr, 64, pp.167~173 (2009)
  20. Smirnoff N, "Hydroxyl radical scavenging activity of compatible solutes", Phytochem, 28, pp.1057~1060 (1989)
  21. Zhang YL, "Sulphated modification of a polysaccharide obtained from fresh persimmon(Diospyros kaki L. )fruit and antioxidant activities of the sulphated derivatives", Food Chem, 127, pp.1084~1090 (2011)
  22. DuBok Choi, "Effect of bamboo oil on antioxidative activity and nitrite scavenging activity", Journal of Industrial and Engineering Chemistry, 14(6), pp.765~770 (2008), 한국공업화학회
  23. Debnath T, "Antioxidant activity of Gardenia jasminoides Ellis fruit extracts", Food Chem, 128, pp.697~703 (2011)
  24. Yen GC, "Antioxidant activity of various tea extracts in relation to their antimutagenicity", J Agric Food Chem, 43, pp.27~32 (1995)
  25. 이관욱, "물과 주정을 이용한 삼채 뿌리와 잎 추출물의 항산화 활성 및 항염증 효과 비교", 한국식품영양과학회지, 43(12), pp.1808~1816 (2014), 한국식품영양과학회
  26. Moure A, "Natural antioxidants from residual sources", Food Chem, 72, pp.145~171 (2001)
  27. Kallel F, "Garlic (Allium sativum L.) husk waste as a potential source of phenolic compounds : influence of extracting solvents on its antimicrobial and antioxidant properties", Industrial Crops Products, 62, pp.34~41 (2014)
  28. García-Herrera P, "Nutrients, phytochemicals and antioxidant activity in wild populations of Allium ampeloprasum L., a valuable underutilized vegetable", Food Res Int, 62, pp.272~279 (2014)
  29. Levine LH, "Antioxidant capacity reduced in scallions grown under elevated CO2 independent of assayed light intensity", Adv Space Res, 44, pp.887~894 (2009)
  30. Simin N, "Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion(Allium flavum L. subsp. flavum, Alliaceae)", LWT-Food Sci Technol, 54, pp.139~146 (2013)
  31. Demirtas I, "Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction", Food Chem, 136, pp.34~40 (2013)
  32. Muanda FN, "Assessment of polyphenolic compounds, in vitro antioxidant and anti-inflammation properties of Securidaca longepedunculata root barks", C R Biol, 333, pp.663~669 (2010)
  33. 류동영, "Characterization of Alkyl Thiosulfinate in Allium hookeri Root Using HPLC-ESI-MS", Applied Biological Chemistry, 56(4), pp.457~459 (2013), 한국응용생명화학회
  34. Kourounakis PN, "Effect on active oxygen species of alliin and Allium sativum(garlic)powder", Res Commun Chem Pathol Pharmacol, 74, pp.249~252 (1991)
  35. Xiao H, "Antioxidant functions of selected Allium thiosulfinates and S-Alk(en)yl-l-cysteine sulfoxides", J Agric Food Chem, 50, pp.2488~2493 (2002)
  36. Liu KL, "DATS reduces LPS-induced iNOS expression, NO production, oxidative stress, and NF-κB activation in RAW 264. 7 macrophages", J Agric Food Chem, 54, pp.3472~3478 (2006)
  37. Dirsch VM, "Effect of allicin and ajoene, two compounds of garlic on inducible nitric oxide synthase", Atherosclerosis, 139, pp.333~339 (1998)
  38. 원준연, "노지 및 시설재배 삼채 뿌리 및 잎의 이화학 성분, DPPH 라디칼 소거능 및 Nitric Oxide 생성 억제효과", 한국식품영양과학회지, 42(9), pp.1351~1356 (2013), 한국식품영양과학회
  39. Yin MC, "Antioxidant activity of several Allium members", J Agric Food Chem, 46, pp.4097~4101 (1998)
  40. Yin MC, "Non-enzymatic antioxidant activity of four organosulfur compounds derived from garlic", J Agric Food Chem, 50, pp.6143~6147 (2002)
  41. Lip Yong Chung, "The Antioxidant Properties of Garlic Compounds: Allyl Cysteine, Alliin, Allicin, and Allyl Disulfide", Journal of Medicinal Food, 9(2), pp.205~213 (2006), 한국식품영양과학회
  42. Bernaert N, "Antioxidant capacity, total phenolic and ascorbate content as a function of the genetic diversity of leek(Allium ampeloprasum var. porrum)", Food Chem, 134, pp.669~677 (2012)
  43. Cervantes MI, "Comparison of antioxidant activity of hydroethanolic fresh and aged garlic extracts and their effects on cerebral ischemia", Food Chem, 140, pp.343~352 (2013)
  44. 정다솜, "추출방법에 따른 양파껍질 추출물의 품질특성 및 항산화활성 비교", 한국식품저장유통학회지, 22(2), pp.267~274 (2015), 한국식품저장유통학회
  45. Dziri S, "Phenolic constituents, antioxidant and antimicrobial activities of rosy garlic(Allium roseum var. odoratissimum)", J Funct Foods, 4, pp.423~432 (2012)
  46. Shin JH, "Short-term heating reduces the anti-inflammatory effects of fresh raw garlic extracts on the LPS-induced production of NO and pro-inflammatory cytokines by downregulating allicin activity in RAW 264. 7 macrophages", Food Chem Toxicol, 58, pp.545~551 (2013)
  47. Kratchanova M, "Composition and properties of biologically active pectic polysaccharides from leek(Allium porrum)", J Sci Food Agric, 90, pp.2046~2051 (2010)

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