추출방법에 따른 핑거루트 추출물의 라디칼 소거 및 α-glucosidase 저해 활성 비교
Comparison of the radical scavenging and α-glucosidase inhibitory activities of fingerroot extracts based on different extraction methods
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초록
핑거루트는 동남아시아가 주요 원산지이지만, 최근에는 국내에서도 많이 재배되고 있다. 본 연구에서는 국내에서 재배된 핑거루트를 80% MeOH, EtOH, acteone 및 열수를 이용하여 추출한 후, 각 추출물에 대해 총페놀성 화합물 함량 평가와 항산화 활성과 관련된 hydoxyl 및 ABTS+ 라디칼 소거능, 항당뇨 활성과 관련된 α-glucosidase 저해능 평가를 수행하였다. 총페놀성 화합물의 함량이 상대적으로 가장 많이 함유되어 있는 핑거루트 80% MeOH 추출물에서 IC50 값이 82.3±2.8 μg/mL로 다른 용매 추출방법에 비해 우수한 hydroxyl 라디칼 소거활성을 확인하였다. ABTS+ 라디칼 소거능 평가에서도 역시 80% MeOH 추출물에서 IC50 값이 75.0±2.4 μg/mL의 가장 우수한 라디칼 소거활성을 확인하였다. 또한, α-glucosidase 저해능 평가에서도 IC50 값이 151.6±3.1 μg/mL의 α-glucosidase 저해활성을 나타내었으며, 다른 추출방법에 비해 우수한 저해활성임을 확인하였다. 이상의 연구 결과, 국산 핑거루트를 효율적으로 추출하기 위해서는 열수보다 유기용매 추출이 효과적이며, 특히 80% MeOH 추출물에서 가장 우수한 라디칼 소거활성 및 α-glucosidase 저해활성을 나타내었다. 또한, 이 활성은 복합적인 추출물 상태의 활성으로 향후 각종 칼럼크로마토그래피 및 기기분석을 이용하여 활성을 나타내는 화합물의 구조 동정이 필요하다고 생각된다. 또한, 현재 동남아산 핑거루트에 대한 연구가 주로 이루어져 있으며, 국산 핑거루트의 대한 세포 및 동물실험 등의 추가적인 활성 기작에 연구를 통하여 천연 기능성 소재 발굴을 위한 기초자료로 활용 가능할 것으로 사료된다.
Abstract
The roots of Boesenbergia rotunda are prominent ingredients in the cuisine of several Asian countries, including Thailand, Malaysia, Indonesia, India, and China. Recently, fingerroot (Boesenbergia rotunda) was successfully cultivated in South Korea. In this study, the radical scavenging and α-glucosidase inhibitory activities of Korean fingerroot extracts obtained using different extraction methods (i.e., organic solvent and hot water extractions) were investigated. More specifically, the antioxidant activities were evaluated using the hydroxyl and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical scavenging assays, while the anti-diabetic effects of the various solvent extracts of fingerroot were tested using the α-glucosidase inhibitory assay. Among the tested samples the 80% methanolic (MeOH) extract showed the most potent activities, with IC50 values of 82.3±2.3 and 75.0±2.4 μg/mL, respectively for the hydroxyl and ABTS+ radical scavenging activities. Also, the 80% MeOH extract exhibited the greatest α-glucosidase inhibitory effects, with an IC50 value of 151.6±3.6 μg/mL. Finally, the total phenolic content of 80% MeOH extract was found to be 106.0±1.7 mg equivalent of gallic acid per g of extract. These results suggest that the 80% MeOH extract of fingerroot can be considered as a new effective source of natural antioxidant and anti-diabetic materials.
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