울릉 섬바디 추출물의 라디칼 소거 및 α-glucosidase 저해 활성
Free radical scavenging and α-glucosidase inhibitory activities of the extracts of Dystaenia takesimana from Ulleung Island
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초록
울릉도에 자생하는 섬바디를 70% MeOH로 침지 추출하여 얻어진 추출물에 대해 각종 유기용매를 활용하여 순차 분획을 실시하였고, 얻어진 추출물 및 분획물을 대상으로 총 페놀 함량, DPPH, ABTS+ 라디칼 소거활성 및 α-glucosidase 저해활성 평가를 수행하였다. DPPH 라디칼 소거활성 평가에서 페놀성 화합물이 가장 많이 함유되어 있는 EtOAc 분획물의 IC50 값이 127.3±2.1 μg/mL을 나타냈으며, ABTS+ 라디칼 소거활성 평가 역시 EtOAc 분획물의 IC50 값이 72.7±1.2 μg/mL으로 가장 우수한 라디칼 소거활성을 나타내었다. 항 당뇨활성과 관련된 α-glucosidase 저해활성 평가에서는 n-hexane 분획물의 IC50 값이 55.5±0.4 μg/mL로 가장 우수한 저해활성을 나타내었으며, 이전 연구 결과와 비교해볼 때 n-hexane 분획물에 우수한 α-glucosidase 저해활성을 나타내는 polyacetylene과 같은 저해활성이 강한 화합물이 다량 함유되어 있을 것으로 추정한다. 다양한 화합물이 함유된 상태의 추출물 시료를 단일물질로 정제할 경우 더욱 우수한 활성의 화합물이 존재할 가능성을 확인하였으며, 향후 이들 추출물의 활성물질 동정을 통한 활성 기작에 대한 연구가 필요하며, 본 연구결과로 보다 우수한 라디칼 소거활성 및 α-glucosidase 저해활성을 가지는 새로운 천연 기능성 소재 발굴을 위한 기초자료로 활용 가능할 것으로 사료된다.
Abstract
In this study, we investigated the free radical scavenging and α-glucosidase inhibitory activities of an aqueous methanolic extract from Dystaenia takesimana sourced from Ulleungdo. Seventy percent of the methanolic extract from D. takesimana was further divided into n-hexane, EtOAc and n-BuOH fractions, which yielded four solvent-soluble portions, namely n-hexane-soluble, EtOAc-soluble, n-BuOH-soluble, and H2O residues. Antioxidant properties were evaluated using the common 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radicals scavenging assays, while the anti-diabetic efficacy of the D. takesimana extract and the solvent-soluble portions were tested using an α-glucosidase assay. All samples exhibited dose-dependent radical scavenging and α-glucosidase inhibitory activities. Among the tested extracts and solvent-soluble fractions, the EtOAc-soluble portion exhibited a much higher radical scavenging activity compared to the other solvent-soluble portions. α-Glucosidase inhibitory IC50 values of 55.5±0.4 and 229.3±2.3 μg/mL were determined for the n-hexane-soluble and EtOAc-soluble portions, respectively. These results suggest that the D. takesimana extract is a potential new source of natural antioxidants and anti-diabetic agents.
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