낙지다리(Penthorum chinense Pursh) 종자 추출물의 항산화 및 α-glucosidase 저해 활성
Antioxidant and α-glucosidase inhibitory effects of Pethorum chinense Purch seed extracts
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초록
낙지다리는 돌나물과에 속하는 식물로 물가나 습지에서 전국적으로 자생하는 대표적인 다년생 초본이다. 본 연구는 낙지다리 종자 추출물의 추출용매에 따른 항산화 및 항당뇨 활성 평가를 통해 야생식물 종자의 경쟁력을 높이는 한편, 종 다양성 보전에 기여하기 위해 진행되었다. 낙지다리 종자 추출물 내 총페놀성 화합물의 함량은 75% EtOH 추출물에서 19.83 mg GAE/g seeds, 총플라보노이드 함량은 75% MeOH 추출물에서 10.89 mg QE/g seeds로 가장 높게 나타났다. 용매에 따른 페놀성 물질은 물 추출물에 비해 유기용매 추출물에서 더 높은 것으로 확인되었다. 낙지다리 종자 추출물의 DPPH 라디컬 소거능의 IC50은 75% MeOH 추출물에서 27.60 μg/mL로 가장 높게 났으며, ABTS 라디컬 소거능의 IC50은 53.54 μg/mL로 75% MeOH 추출물에서 가장 높게 나타났다. 낙지다리 종자 추출물의 총항산화 능력은 30.87 μmol AAE/g으로 75% EtOH 추출물에서 가장 높게 나타났다. 낙지다리 종자 추출물의 α-glucosidase 저해활성 IC50은 75% EtOH 추출물에서 46.47 μg/mL로 가장 높게 나타났다. 본 연구결과를 통해 풍부한 페놀성 물질을 가진 낙지다리 종자의 유기용매 추출물이 항산화와 항당뇨 효과를 나타낸다는 것을 확인하였으며, 이는 효과적인 건강기능식품 소재 및 천연물 의약품으로서 이용할 수 있을 것으로 기대한다.
Abstract
Penthorum chinense Pursh is a plant belonging to the Crassulaceae family and is a representative perennial herb that grows nationwide in watersides and wetlands. This study was conducted to enhance the competitiveness of wild plant seeds and contribute to the conservation of species diversity through the evaluation of antioxidant and antidiabetic activities of P. chinense seed extracts based on extraction solvents. The total phenolics content was 19.83 mg GAE/g seeds in the 75% ethanol extract, whereas the flavonoid content was the highest (10.89 mg QE/g seeds) in the 75% methanol extract. Total phenolicss content was higher in the organic solvent extract than in the water extract. The DPPH radical scavenging activity was the highest (IC50 27.60 μg/mL) in the 75% methanol extract, and the ABTS radical scavenging activity was the highest (IC50 53.54 μg/mL) in the 75% methanol extract. Total antioxidant capacity was the highest (30.87 μmol AAE/g) in the 75% ethanol extract. The α-glucosidase-inhibitory activity was the highest (IC50 46.47 μg/mL) in the 75% ethanol extract. From the results of this study, it was confirmed that P. chinense seed extracts, which possess high phenolics content, exhibit antioxidant and antidiabetic effects, and could be used as an effective health functional food material and natural medicine.
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