Promoting Effects of Soybean Methanol Extract on the Melanogenesis and Tyrosinase Activity
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The promoting effect of soybean (Glicine maxim) methanol extract (GMME) on the melanogenesis and tyrosinase activity was investigated in melan-a cells, and 3-isobuty-1-methylxanthine (IBMX) was used as positive control (PC). In antioxidative ability analysis, GMME was found to contain 53.7 mg/g of polyphenol and 62.7 mg/g of flavonoid in total. Electron-donating abilities of BHT and GMME at 1,000 μg/mL were 68.4% and 14.4%, respectively. In morphological observation of melan-a cells, as the treatment concentration increased, more dendritic development and melanin accumulation observed. Melanin contents of GMME and IBMX treatment groups were significantly (p⁄0.001) higher by 51.2% and 31.6% at the concentration of 12.5 μg/mL, respectively, compared to the control group. Intra-cellular tyrosinase activities of GMME and PC treatment groups were significantly (p⁄0.001) higher by 90.0% and 54.1% at the concentration of 12.5 μg/mL, respectively, compared to the control group. Cell-extracted tyrosinase activities of GMME and PC treatment groups were higher by 12.5% (p⁄0.01) and 27.2% (p⁄0.001) at the concentration of 12.5 μg/mL,respectively, compared to the control group. GMME showed a promoting effect on melanogenesis and tyrosinase activity. From these results, it can be speculated that GMME could be applied to improve hair graying.
Abstract
The promoting effect of soybean (Glicine maxim) methanol extract (GMME) on the melanogenesis and tyrosinase activity was investigated in melan-a cells, and 3-isobuty-1-methylxanthine (IBMX) was used as positive control (PC). In antioxidative ability analysis, GMME was found to contain 53.7 mg/g of polyphenol and 62.7 mg/g of flavonoid in total. Electron-donating abilities of BHT and GMME at 1,000 μg/mL were 68.4% and 14.4%, respectively. In morphological observation of melan-a cells, as the treatment concentration increased, more dendritic development and melanin accumulation observed. Melanin contents of GMME and IBMX treatment groups were significantly (p⁄0.001) higher by 51.2% and 31.6% at the concentration of 12.5 μg/mL, respectively, compared to the control group. Intra-cellular tyrosinase activities of GMME and PC treatment groups were significantly (p⁄0.001) higher by 90.0% and 54.1% at the concentration of 12.5 μg/mL, respectively, compared to the control group. Cell-extracted tyrosinase activities of GMME and PC treatment groups were higher by 12.5% (p⁄0.01) and 27.2% (p⁄0.001) at the concentration of 12.5 μg/mL,respectively, compared to the control group. GMME showed a promoting effect on melanogenesis and tyrosinase activity. From these results, it can be speculated that GMME could be applied to improve hair graying.
참고문헌 (32)
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