항효모성 물질 생산을 위한 Rahnella aquatilis AY2000 균주의 생육특성
Characteristics of Rahnella aquatilis Strain AY2000 for an Anti-Yeast Substance Production.
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초록
Rahnella aquatilis AY2000 has an unique characteristic which produces an anti-yeast substance (AYS). The AYS of the strain AY2000 was always secreted on agar plate, however, its activity in liquid culture was labile upon storage of the medium. In this paper, cultural conditions of the strain AY2000 for the AYS production were investigated in liquid culture, and minimal inhibitory concentration (MIC) against Saccharomyces cerevisiae was determined for the AYS activity. MIC of the AYS cultured in PYG broth at 25oC for 24 hr was 23.5 μg/mL, however, that in MYCS (pH 5.5) broth at the same condition was 15.5 μg/mL. The activity of the AYS had increased rather in MYCS broth excluded NH4-citrate than in the same broth contained NH4-citrate, and MIC of the AYS produced in MYCS broth without NH4-citrate was 15.5 μg/mL. When the strain AY2000 was maintained in MYCS broth without NH4-citrate but added 100 μM FeCl3, the activity of the AYS had increased and its MIC was 7.8 μg/mL. MIC of the AYS was 7.8 μg/mL after the strain AY2000 was cultured in MYCS broth containing 100 μM FeCl3 without NH4-citrate, however, its MIC was 31.3 μg/mL after 48-60 hr culture in the same broth.
Abstract
Rahnella aquatilis AY2000 has an unique characteristic which produces an anti-yeast substance (AYS). The AYS of the strain AY2000 was always secreted on agar plate, however, its activity in liquid culture was labile upon storage of the medium. In this paper, cultural conditions of the strain AY2000 for the AYS production were investigated in liquid culture, and minimal inhibitory concentration (MIC) against Saccharomyces cerevisiae was determined for the AYS activity. MIC of the AYS cultured in PYG broth at 25oC for 24 hr was 23.5 μg/mL, however, that in MYCS (pH 5.5) broth at the same condition was 15.5 μg/mL. The activity of the AYS had increased rather in MYCS broth excluded NH4-citrate than in the same broth contained NH4-citrate, and MIC of the AYS produced in MYCS broth without NH4-citrate was 15.5 μg/mL. When the strain AY2000 was maintained in MYCS broth without NH4-citrate but added 100 μM FeCl3, the activity of the AYS had increased and its MIC was 7.8 μg/mL. MIC of the AYS was 7.8 μg/mL after the strain AY2000 was cultured in MYCS broth containing 100 μM FeCl3 without NH4-citrate, however, its MIC was 31.3 μg/mL after 48-60 hr culture in the same broth.
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