Trehalose에 의한 Rahnella aquatilis AY2000 균주가 생산하는 항효모성 물질의 활성보호
Trehalose Protects Activity of Anti-Yeast Substance Produced by Rahnella aquatilis Strain AY2000
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Rahnella aquatilis strain AY2000 produces an anti-yeast substance (AYS), however activity of the AYS tends to be decreased by heat. To investigate whether trehalose can protect AYS activity against heat, comparative studies on the AYS with and without trehalose were performed. After heat treatment at high temperatures (50℃-70℃), the AYS with trehalose had higher activity than the AYS without trehalose had. In case of AYS with trehalose (0.3-0.9M), activity of the AYS could be determined at ranging from 7.8 μg/mL to 31.3 μg/mL on S. cerevisiae by MTT method. Consequently, activity of the AYS after heating was well maintained by trehalose.
Abstract
Rahnella aquatilis strain AY2000 produces an anti-yeast substance (AYS), however activity of the AYS tends to be decreased by heat. To investigate whether trehalose can protect AYS activity against heat, comparative studies on the AYS with and without trehalose were performed. After heat treatment at high temperatures (50℃-70℃), the AYS with trehalose had higher activity than the AYS without trehalose had. In case of AYS with trehalose (0.3-0.9M), activity of the AYS could be determined at ranging from 7.8 μg/mL to 31.3 μg/mL on S. cerevisiae by MTT method. Consequently, activity of the AYS after heating was well maintained by trehalose.
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