항진균성 활성 Bacillus sp.의 분리 및 생산 조건
Isolation and Cultural Conditions of an with Antifungal Activity Bacillus sp.
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Abstract
An antifungal antibiotic-producing bacterium was isolated from soil and identified as Bacillus sp. CJ-1. The culture supernatant was found to have a strong and stable antifungal activity against Colletotrichum gloeosporioides. Culture conditions for the maximum antifungal activity were examined. Glucose and yeast extract were selected as the best carbon and nitrogen sources. The optimum C/N ratio was 3. The optimum temperature and initial pH were determined as 35℃ and 6.0, respectively. Under these conditions, the production for the antibiotic was maximized at 72 hr at 35℃ after cultivation. Microscopic observation showed that the culture supernatant of Bacillus sp. CJ-1 had a strong inhibitory activity on the mycelial growth of the test strain at above 12.5 μL mL-1 of concentration.
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