장수상황버섯 균사체를 이용한 한약재의 고체발효 및 메탄올 추출물의 트롬빈 저해 활성과 항산화 활성
Solid Fermentation of Medicinal Herb Using Phellinus baumii Mycelium and Anti-thrombin and Antioxidation Activity of its Methanol Extract.
2
조회
0
다운로드
11
인용
0
참고문헌
초록
To produce bioactivitystrengthen medicinal herbs, the 36 medicinal herbs which have antioxidation or blood circulation activity, were solid fermented using Phellinus baumii mycelium. Most of medicinal herbs, except Chrysanthemum indicum (flower), Zizyphus jujuba Miller (fructus), Aconitum koreanum R. Raymond (root), Magnolia denudata(flower), and Polygonatum sibiricum Redt (root bark), showed good fermentation at 25oC for 20 days under 90% of relative humidity. The poor fermentations of the herbs could be explained by lack of nutrient, structural rigidity, and the content of antifungal substance. After fermentation, the average water content of herbs were increased to 67.21±11.43% from 30.84±15.67%, but the average pH and average methanol extraction ratio were slightly decreased to 11.16±7.06% and 4.83±0.73 from 13.91±12.22% and 5.06±0.87, respectively. The analysis of thrombin inhibition and DPPH scavenging activity of the methanol extracts of herbs showed that thrombin inhibition activities of the fermented Drynaria fortunei Kunze, Melia azedarach var. japonica, Prunus persica and Orostachys japonicus, and DPPH scavenging activities of the fermented Polygala tenuifolia, Scrophularia buergeriana, Angelica dahurica, Drynaria fortunei Kunze, Cyperus rotundus, and Boschniakia rossica were increased as compared with those activities of non-fermented its cognate herbs. Our results suggest that the production of bioactivity-strengthen medicinal herbs is possible by solid fermentation of Phellinus baumii mycelium, as fermented Drynaria fortunei Kunze showed increased antioxidant and thrombin inhibitory activities than those of non-fermented herbs.
Abstract
To produce bioactivitystrengthen medicinal herbs, the 36 medicinal herbs which have antioxidation or blood circulation activity, were solid fermented using Phellinus baumii mycelium. Most of medicinal herbs, except Chrysanthemum indicum (flower), Zizyphus jujuba Miller (fructus), Aconitum koreanum R. Raymond (root), Magnolia denudata(flower), and Polygonatum sibiricum Redt (root bark), showed good fermentation at 25oC for 20 days under 90% of relative humidity. The poor fermentations of the herbs could be explained by lack of nutrient, structural rigidity, and the content of antifungal substance. After fermentation, the average water content of herbs were increased to 67.21±11.43% from 30.84±15.67%, but the average pH and average methanol extraction ratio were slightly decreased to 11.16±7.06% and 4.83±0.73 from 13.91±12.22% and 5.06±0.87, respectively. The analysis of thrombin inhibition and DPPH scavenging activity of the methanol extracts of herbs showed that thrombin inhibition activities of the fermented Drynaria fortunei Kunze, Melia azedarach var. japonica, Prunus persica and Orostachys japonicus, and DPPH scavenging activities of the fermented Polygala tenuifolia, Scrophularia buergeriana, Angelica dahurica, Drynaria fortunei Kunze, Cyperus rotundus, and Boschniakia rossica were increased as compared with those activities of non-fermented its cognate herbs. Our results suggest that the production of bioactivity-strengthen medicinal herbs is possible by solid fermentation of Phellinus baumii mycelium, as fermented Drynaria fortunei Kunze showed increased antioxidant and thrombin inhibitory activities than those of non-fermented herbs.
참고문헌 (29)
- 박승춘, "Phellinus linteus, Phellinus baumii 및 Phellinus gilvus의형태, 일반성분, 원소분석 및 무기성분 함량의 비교", 대한수의학회지, 43(3), pp.423~428 (2003), 대한수의학회
- Han, Y. S., "Acute oral toxicity studies of extract of sanghwang mushroom (Phellinus linteus)", J. Appl. Pharmacol, 9, pp.46~50 (2001)
- 김순동, "귤피 및 녹차추출물에서 배양한 소나무잔나비버섯(Fomitopsis pinicola) 균사체의 유리아미노산 및 다당류 함량", 한국식품저장유통학회지, 12(4), pp.379~386 (2005), 한국식품저장유통학회
- Ji, J. H., "Antimutagenic and cytotoxicity effects of Phellinus linteus extracts", J. Kor. Soc. Food Sci. Nutr, 30, pp.331~337
- Kang, I. J., "Biochemical and histological effects of Phellinus linteus methanol extract on liver lipid metabolism of rats fed CC14 and high fat", J. Kor. Soc. Food Sci. Nutr, 30, pp.331~337 (2001)
- Kim, D. H., "Effect of artificially cultured Phellinus linteus on harmful intestinal bacteria enzymes and rat intestinal aglucosidase", J. Fd. Hyg. Safety, 13, pp.20~23 (1998)
- Kim, J. S., "Blood anticoagulation active substance from Phellinus linteus mycelia", J. Kor. Soc. Food Sci. Nutr, Spring symposium, pp.224~ (1998)
- 이재동, "Development of Species-specific Primers for Rapid Detection of Phellinus linteus and P. baumii", Mycobiology, 33(2), pp.104~108 (2005), 한국균학회
- Kim S. H., "Anti-inflammatory and related pharmacological activities of the n-BuOH subfraction of mushroom Phellinus linteus", J. Ethnopharmacol, pp.141~146
- 권대준, "추출방법에 따른 상황버섯 추출물의 항산화활성 및 생물학적 특성", Journal of the Korean Society for Applied Biological Chemistry, 49(2), pp.91~96 (2006), 한국응용생명화학회
- 권석형, "버섯 균사체에 의한 암세포 성장억제 효과", 한국식품영양학회지, 16(1), pp.15~21 (2003), 한국식품영양학회
- 이창환, "감귤 주스 착즙박을 이용하여 재배된 버섯균사체의 성분", 한국식품과학회지, 39(2), pp.128~132 (2007), 한국식품과학회
- 강영주, "감귤 주스 착즙박을 이용하여 재배된 버섯균사체의 용매추출에 의한 휘발성 성분", 한국식품저장유통학회지, 14(4), pp.351~355 (2007), 한국식품저장유통학회
- Lee, J. H., "Effects of mycelial culture of Phellinus linteus on ethanol-induced gastric ulcer in rats", Phytother. Res, 20, pp.396~402 (2006)
- Lee. S. Y., "Optimization in bioconversion of starch processing waste to Phellinus linteus mycelia using response surface analysis", J. Kor. Soc. Env. Eng, Autumn symposium, pp.562~565 (2006)
- 류하나, "식용 식물자원으로부터 활성물질의 탐색-XVIII. 상황버섯(Phellinus linteus) 자실체로부터 Ergosterol 유도체의 분리", Journal of the Korean Society for Applied Biological Chemistry, 50(1), pp.57~62 (2007), 한국응용생명화학회
- 문형철, "마늘 첨가 복합배지에서 배양된 영지 균사체의 면역 증진 효과", 한국약용작물학회지, 12(1), pp.24~30 (2004), 한국약용작물학회
- 박은미, "인삼박으로 배양된 버섯 균사체 추출물이 암세포 증식에 미치는 영향", 한국식품영양과학회지, 34(3), pp.323~329 (2005), 한국식품영양과학회
- Park. J. H., "An antioxidant hispidin from the mycelial cultures of Phellinus linteus", Arch. Pharm. Res, 27, pp.615~618 (2004)
- Park, J. H., "A ß-secretase (BACE1) inhibitor hispidin from the mycelial cultures of Phellinus linteus", Planta Med, 70, pp.143~146 (2004)
- Pyo, M. Y., "Effects of Phellinus linteus extracts on the humoral immune response in normal and cyclophosphamide-treated mice", J. Appl. Pharmacol, 9, pp.194~200 (2001)
- Rhee, Y. K., "In vitro and in vivo antitumor activity of the fruit body of Phellinus linteus", Kor. J. Food Sci. Technol, 32, pp.477~480 (2000)
- 류희영, "시판 전통주의 항균, 항산화 및 항혈전 활성 평가", 한국미생물·생명공학회지, 35(3), pp.238~244 (2007), 한국미생물·생명공학회
- 손호용, "생마 신선편이 제조를 위한 천연 향신료의 항균, 항산화 및 항혈전 활성 평가", 생명과학회지, 17(5), pp.652~657 (2007), 한국생명과학회
- 손호용, "산사자 추출물의 트롬빈 저해활성", 생명과학회지, 17(4), pp.535~539 (2007), 한국생명과학회
- Tae-Yong Shin, "Phellinus baumii Inhibits Immediate-type Allergic Reactions", Natural Product Sciences, 12(4), pp.232~236 (2006), 한국생약학회
- 손호용, "시판 쌀 메탄올 추출물의 항혈전 및 항산화 활성", 한국식품영양과학회지, 34(5), pp.593~598 (2005), 한국식품영양과학회
- 손호용, "메밀 종자의 항트롬빈 활성과 혈전증 예방효과", 한국식품영양과학회지, 35(2), pp.132~138 (2006), 한국식품영양과학회
- Song. K. S., "Secondary metabolites from the mycelial culture broth of Phellinus linteus", Agri. Chem. Biotechnol, 37, pp.100~104 (1994)