Properties of Antimicrobial Substances Produced by Bacillus species Isolated from Rice Straw
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Two hundred bacilli were isolated from rice straw, and 3 strains showing strong inhibitory activities against Bacillus cereus ATCC14579 were selected for further analyses. RSC15 was identified as B. licheniforms, and RSC26 and RSC42 were identified as B. amyloliquefaciens by molecular identification methods. The inhibitory activities were heat stable, and half of the activity was retained for 20 min at 100℃. SDSPAGE analyses of the culture supernatant indicated that 2 different kinds of antibacterial substances were present with sizes below 3.5 kDa. Antibacterial substances produced by B. licheniformis RSC15 were partially purified by column chromatography, and the specific activity increased from 955.0 AU/mg to 6,400 AU/mg.
Abstract
Two hundred bacilli were isolated from rice straw, and 3 strains showing strong inhibitory activities against Bacillus cereus ATCC14579 were selected for further analyses. RSC15 was identified as B. licheniforms, and RSC26 and RSC42 were identified as B. amyloliquefaciens by molecular identification methods. The inhibitory activities were heat stable, and half of the activity was retained for 20 min at 100℃. SDSPAGE analyses of the culture supernatant indicated that 2 different kinds of antibacterial substances were present with sizes below 3.5 kDa. Antibacterial substances produced by B. licheniformis RSC15 were partially purified by column chromatography, and the specific activity increased from 955.0 AU/mg to 6,400 AU/mg.
참고문헌 (22)
- Schallmey M, "Developments in the use of Bacillus species for industrial production", Can. J. Microbiol, 50, pp.1~17 (2004)
- 이재준, "Bacillus subtilis DJI을 이용하여 제조한 된장의 항산화효과", 한국식품저장유통학회지, 16(4), pp.554~561 (2009), 한국식품저장유통학회
- Hwang A Lee, "Isolation of Bacillus amyloliquefaciens Strains with Antifungal Activities from Meju", Preventive Nutrition and Food Science, 17(1), pp.64~70 (2012), 한국식품영양과학회
- Gálvez A, "Bacteriocinbased strategies for food biopreservation", Food Microbiol, 120, pp.51~70 (2007)
- Stein T, "Bacillus subtilis antibiotics: structures, syntheses and specific functions", Mol. Microbiol, 56, pp.845~857 (2005)
- Kindoli, Salum, "Properties of Bac W42, a Bacteriocin Produced by Bacillus subtilis W42 Isolated from Cheonggukjang", Journal of Microbiology and Biotechnology, 22(8), pp.1092~1100 (2012), 한국미생물·생명공학회[DOI]
- 류샤오밍, "Properties of Antimicrobial Substances Produced by Bacillus amyloliquefaciens CJW15 and Bacillus amyloliquefaciens SSD8", 한국미생물·생명공학회지, 44(1), pp.9~18 (2016), 한국미생물·생명공학회[DOI]
- Yu GY, "Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani", Soil Biol. Biochem, 34, pp.955~963 (2002)
- Thaniyavarn J, "Production and characterization of biosurfactants from Bacillus licheniformis F2.2", Biosci. Biotechnol. Biochem, 67, pp.1239~1244 (2003)
- Kalai-Grami L, "Production and identification of iturin A lipopeptide from Bacillus methyltrophicus TEB1 for control of Phoma tracheiphila", J. Basic Microbiol, 56, pp.864~871 (2016)
- From C, "Food poisoning associated with pumilacidin-producing Bacillus pumilus in rice", Int. J. Food Microbiol, 115, pp.319~324 (2007)
- Abdel-Mawgoud AM, "Characterization of surfactin produced by Bacillus subtilis isolate BS5", Appl. Biochem. Biotechnol, 150, pp.289~303 (2008)
- Compaoré CS, "Co-production of surfactin and a novel bacteriocin by Bacillus subtilis subsp. subtilis H4 isolated from Bikalga, an African alkaline Hibiscus sabdariffa seed fermented condiment", Int. J. Food Microbiol, 162, pp.297~307 (2013)
- 김평일, "Production of Biosurfactant Lipopeptides Iturin A, Fengycin, and Surfactin A from Bacillus subtilis CMB32 for Control of Colletotrichum gloeosporioides", Journal of Microbiology and Biotechnology, 20(1), pp.138~145 (2010), 한국미생물·생명공학회
- Hathout Y, "Kurstakins:a new class of lipopeptides isolated from Bacillus thuringiensis", J. Nat. Prod, 63, pp.1492~1496 (2000)
- Spelhaug SR, "Inhibition of foodborne bacterial pathogens by bacteriocins from Lactococcus lactis and Pediococcus pentosaceous", J. Food Protec, 52, pp.856~862 (1989)
- Kamoun F, "Purification, amino acid sequence and characterization of Bacthuricin F4, a new bacteriocin produced by Bacillus thuringiensis", J. Appl. Microbiol, 98, pp.881~888 (2005)
- Martínez AM, "Revision of the taxonomic position of the xylanolytic Bacillus sp. MIR32 reidentified as Bacillus halodurans and plasmid-mediated transformation of B. halodurans", Extremophiles, 6, pp.391~395 (2002)
- Xiaoming Liu, "Properties of a Bacteriocin Produced by Bacillus subtilis EMD4 Isolated from Ganjang (Soy Sauce)", Journal of Microbiology and Biotechnology, 25(9), pp.1493~1501 (2015), 한국미생물·생명공학회[DOI]
- Kwon GH, "Development of a RAPD-PCR method for identification of Bacillus species isolated from cheonggukjang", Int. J. Food Microbiol, 129, pp.282~287 (2009)
- Schägger H, "Tricine–SDS-PAGE", Nature Protocols, 1, pp.16~22 (2006)
- Mora I, "Antimicrobial peptide genes in Bacillus strains from plant environments", Int. Microbiol, 14, pp.213~223 (2011)