Bacillus cereus를 억제하는 Bacillus subtilis HH28의 항균물질정제와 특성규명
Purification and Characterization of an Antimicrobial Substance from Bacillus subtilis HH28 Antagonistic to Bacillus cereus
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초록
A bacterium producing antimicrobial substance was isolated from cheonggukjang. The bacterium was identified as a strain of Bacillus subtilis by 16S rDNA sequencing and designated as Bacillus subtilis HH28. The antimicrobial substance produced from Bacillus subtilis HH28 was purified by 0-80% ammonium sulfate precipitation, DEAE-sepharose FF column chromatography, and Sephacryl S-200 HR gel chromatography. The molecular weight of the purified antimicrobial substance was estimated to be approximately 3,500 Da using Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis and direct detection analysis. Antimicrobial substance from B. subtilis HH28 not only inhibited B. cereus, but also Listeria monocytogenes and Vibrio parahaemolyticus. The purified antimicrobial substance was stable at 40-80oC, and between pH 2 and 8. Antimicrobial activity of the purified substance was completely destroyed by treatment of protease, proteinase K, and pronase E, indicating that it is proteinaceous.
Abstract
A bacterium producing antimicrobial substance was isolated from cheonggukjang. The bacterium was identified as a strain of Bacillus subtilis by 16S rDNA sequencing and designated as Bacillus subtilis HH28. The antimicrobial substance produced from Bacillus subtilis HH28 was purified by 0-80% ammonium sulfate precipitation, DEAE-sepharose FF column chromatography, and Sephacryl S-200 HR gel chromatography. The molecular weight of the purified antimicrobial substance was estimated to be approximately 3,500 Da using Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis and direct detection analysis. Antimicrobial substance from B. subtilis HH28 not only inhibited B. cereus, but also Listeria monocytogenes and Vibrio parahaemolyticus. The purified antimicrobial substance was stable at 40-80oC, and between pH 2 and 8. Antimicrobial activity of the purified substance was completely destroyed by treatment of protease, proteinase K, and pronase E, indicating that it is proteinaceous.
참고문헌 (32)
- Andersson A, "What problems does the food industry have with the spore-forming pathogens Bacillus cereus and Clostridium perfringens?", Int. J. Food Microbiol, 28, pp.45~155 (1995)
- Abriouel H, "Diversity and applications of Bacillus bacteriocins", FEMS Microbiol. Rev, 35, pp.201~232 (2011)
- Bhunia AK, "Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulfate-polyacrylamide gel electrophoresis", J. Ind. Microbiol, 2, pp.319~322 (1987)
- Edman P, "A protein sequenator", Eur. J. Biochem, 1, pp.80~91 (1967)
- Yaoqi Guo, "Identification of a New Bacillus licheniformis Strain Producing a Bacteriocin-Like Substance", The Journal of Microbiology, 50(3), pp.452~458 (2012), 한국미생물학회
- He Z, "Isolation and identification of a Paenibacillus polymyxa strain that coproduces a novel lantibiotic and polymyxin", Appl. Environ. Microbiol, 73, pp.168~178 (2007)
- Mah JH, "Bacteriocin with a broad antimicrobial spectrum, produced by Bacillus sp. isolated from Kimchi", J. Microbiol. Biotechnol, 11, pp.577~584 (2001)
- Jack RW, "Bacteriocin of Gram-positive bacteria", Microbiol. Rev, 59, pp.171~200 (1995)
- 정우진, "Stability and Antibacterial Activity of Bacteriocins Produced by Bacillus thuringiensis and Bacillus thuringiensis ssp. kurstaki", Journal of Microbiology and Biotechnology, 18(11), pp.1836~1841 (2008), 한국미생물·생명공학회
- Salum Kindoli, "Properties of a Bacteriocin from Bacillus subtilis H27 Isolated from Cheonggukjang", Food Science and Biotechnology, 21(6), pp.1745~1751 (2012), 한국식품과학회[DOI]
- Kindoli, Salum, "Properties of Bac W42, a Bacteriocin Produced by Bacillus subtilis W42 Isolated from Cheonggukjang", Journal of Microbiology and Biotechnology, 22(8), pp.1092~1100 (2012), 한국미생물·생명공학회[DOI]
- Klaenhammer TR, "Genetics of bacteriocins produced by lactic acid bacteria", FEMS Microbiol. Rev, 12, pp.39~85 (1993)
- Lawton EM, "Two-peptide lantibiotics: a medical perspective", Mini. Rev. Med. Chem, 7, pp.1236~1247 (2007)
- Lee NK, "Isolation and characterization of a novel analyte from Bacillus subtilis SC-8 antagonistic to Bacillus cereus", J. Biosci. Bioeng, 110, pp.298~303 (2010)
- Marx R, "Structure of the Bacillus subtilis peptide antibiotic subtilosin A determined by 1H-NMR and matrix assisted laser desorption", ionization timeof-flight mass spectrometry, 2001, pp.20, 501~506
- Nicolas GG, "Production, purification, sequencing, and activity spectra of mutacins D-123.1and F-59.1", BMC. Microbiol, 11, pp.69~ (2011)
- Ohnishi ST, "A simplified method of quantitation protein using the biuret and phenol reagents", Anal. Biochem, 86, pp.193~200 (1978)
- Paik SH, "Identification and characterization of the structural and transporter genes for, and the chemical and biological properties of sublancin 168, a novel lantibiotic produced by Bacillus subtilis 168", J. Biol. Chem, 273, pp.23134~23142 (1998)
- Pattnaik P, "Purification and characterization of a bacteriocin-like compound (lichenin) produced anaerobically by Bacillus licheniformis isolated from water buffalo", J. Appl. Microbiol, 91, pp.636~645 (2001)
- Sass P, "The lantibiotic mersacidin is a strong inducer of the cell wall stress response of Staphylococcus aureus", BMC. Microbiol, 8, pp.186~197 (2008)
- Schägger H, "Tricine-SDS-PAGE", Nature protocols, ~ (2006)
- Sonenshein AL, "Bacillus subtilis and other gram positive bacteria: biochemistry, physiology and molecular genetics", pp.120~142 (1993), ASM press
- Stein T, "Two different lantibiotic like peptides originate from the ericin gene cluster of Bacillus subtilis A1", 3, 2002, pp.184, 1703~1711
- Stein T, "Whole-cell matrix-assisted laser desorption", ionization mass spectrometry for rapid identification of bacteriocin, Rapid Commun Mass Spectrom, pp.2008, 22[DOI]
- Sutyak KE, "Spermicidal activity of the safe natural antimicrobial peptide subtilosin", Infect Dis. Obstet. Gynecol, 8, pp.1~6 (2008)
- Sirtori LR, "Mode of action of antimicrobial peptide P45 on Listeria monocytogenes", J. Basic Microbiol, 48, pp.393~400 (2008)
- Sirtori LR, "Purification and partial characterization of an antimicrobial peptide produced by Bacillus sp. strain P45, a bacterium from the Amazon basin fish Piaractus mesopotamicus", J. Gen. Appl. Microbiol, 52, pp.357~363 (2006)
- Turnbull PC, "Properties and production characteristics of vomiting, diarrheal, and necrotizing toxins of Bacillus cereus", Am. J. Clin. Nutr, 32, pp.219~228 (1979)
- Tagg J, "Assay system for bacteriocin", Appl. Environ. Microbiol, 21, pp.943~948 (1971)
- Xie J, "Isolation and characterization of a bacteriocin produced by an isolated Bacillus subtilis LFB112 that exhibits antimicrobial activity against domestic animal pathogens", Afr. J. Biotechnol, 8, pp.5611~5619 (2009)
- Xu D, "Inhibitory activity of a novel antibacterial peptide AMPNT-6 from Bacillus subtilis against Vibrio parahaemolyticus in shrimp", Food Control, 30, pp.58~61 (2013)
- 양은주, "Bacillus subtilis MJP1이 생산하는 Bacteriocin-Like Substances", 한국미생물·생명공학회지, 35(4), pp.339~346 (2007), 한국미생물·생명공학회
