목형 잎 추출물의 항산화 활성과 멜라닌 생합성에 대한 저해활성
Antioxidative, and Inhibitory Activities on Melanogenesis of Vitex negundo L. Leaf Extract
0
조회
0
다운로드
12
인용
0
참고문헌
키워드
초록
본 연구에서는 목형 잎 추출물의 항산화 활성, 성분분석그리고 mushroom tyrosinase 저해활성 측정, α-MSH로 유도된 세포 내 멜라닌 생합성에 대한 저해활성에 관한 연구를 수행하였다. 목형 잎 추출물의 자유라디칼 (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성 (FSC50)은 에틸아세테이트 분획에서 14.51 μg/ml, 아글리콘 분획에서 13.96 μg/ml로측정되었다. Luminol-의존성 화학발광법을 이용한 Fe3+-EDTA/H2O2 계에서 생성된 활성산소종 (reactive oxygen species, ROS)에 대한 목형 잎 추출물의 총항산화능은 아글리콘 분획에서 0.22 μg/ml로, 아글리콘 분획에서 가장 큰 활성을 나타내었다. 목형 잎 추출물에 대하여 rose-bengal로 증감된 1O2에 의한 적혈구 파괴에 대한 세포보호 효과는 모든분획에서 농도 의존적(1~50 μg/ml)으로 증가하였으며, 특히아글리콘 분획에서 지용성 항산화제인 (+)-α-tocopherol 보다우수한 세포보호 활성이 있는 것으로 나타났다. Mushroom tyrosinase의 활성 저해 효과(IC50)를 측정한 결과 에틸아세테이트 분획(IC50 = 48.58 μg/ml)에서 우수한 효과를나타났고, α-MSH로 유도된 세포 내 멜라닌 합성 저해 활성을 측정한 결과 에틸아세테이트 분획물의 50 μg/ml 농도에서 41.80% 저해활성을 나타내었다. TLC, HPLC 및 LC/ESIMS를이용한 성분분석 결과 에틸아세테이트 분획 중에luteolin, isoorientin이 존재함을 확인하였다. 이상의 결과들로부터 목형 잎 추출물은 활성산소종을 소거하는 항산화제로 이용가능하며, 특히 mushroom tyrosinase 저해효과와 α-MSH로 유도된 멜라닌 생합성 저해 효과로부터 에틸아세테이트 분획 중 luteolin 및 isoorientin을 새로운 미백 화장품의 원료로써 이용가능성을 알 수 있었다.
Abstract
The aim of this study was to evaluate various aspects of Vitex negundo L. leaf extract, such as the antioxidative activity, tyrosinase inhibitory effects, and inhibitory activities on α-MSH induced melanogenesis, and active component analysis. The DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging activities (FSC50) of the ethyl acetate fraction and aglycone fraction of V. negundo L. leaf extract were 14.51 μg/ml and 13.96 μg/ml, respectively. A luminol-dependent chemiluminescence assay revealed that the reactive oxygen species (ROS) scavenging activity (OSC50) of the aglycone fraction of V. negundo L. leaf extract on ROS generated in an Fe3+-EDTA/H2O2 system was the most prominent at 0.22 μg/ml. The protective effects of the extracts fractions of V. negundo L. leaf against the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner (1~50 μg/ml). In particular, there were greater protective effects of the aglycone fraction on the cellular membrane than that of the fat-soluble antioxidant (+)-α-tocopherol. The inhibitory effects (IC50) on mushroom tyrosinase were the highest for the ethyl acetate fraction (IC50 = 48.58 μg/ml). The inhibitory effect on α-MSH induced melanogenesis in B16 melanoma cells was 41.80% at 50 μg/ml of ethyl acetate fraction. Active component analyses by TLC, HPLC and LC/ESI-MS revealed luteolin and isoorientin. These results indicate that V. negundo L. leaf extract can be used as an antioxidant for ROS scavenging. Particularly, the luteolin and isoorientin of the ethyl acetate fraction may be applicable to new whitening cosmetics because of its inhibitory effect on mushroom tyrosinase and α-MSH induced melanogenesis in B16 melanoma cells.
참고문헌 (37)
- 박수남, "라벤더 발효추출물의 항산화 활성과 성분 분석", 대한화장품학회지, 35(2), pp.125~134 (2009), 대한화장품학회
- Ando, H., "Melanosomes are transferred from melanocyte to keratinocytes through the processes of packaging, release, uptake, and dispersion", J. Invest. Dermatol, 132, pp.1222~1229 (2012)
- Aruoma, O. I., "Damage to the bases in DNA induced by hydrogen peroxide and ferric ion chelates", J. Biol. Chem, 264, pp.20509~20512 (1989)
- Choi, B. W., "Screening of the tyrosinase inhibitorys from marine algae and medicinal plants", Korean J. Pharmacogn, 29, pp.237~242 (1998)
- 최미영, "Whitening Activity of Luteolin Related to the Inhibition of cAMP Pathway in α-MSH-stimulated B16 Melanoma Cells", Archives of Pharmacal Research, 31(9), pp.1166~1171 (2008), 대한약학회
- Ciapetti, G., "In vitro evaluation of cell", biomaterial interaction by MTT assay, 1993, pp.14, 359~364
- Es-Safi, N. E., "Application of ESI", MS, CID, MS to the fragmentation study of eriodictyol 7-O-glucosyl-(1→2)-glucoside and luteolin 7-O-glucosyl-(1→2)-glucoside, pp.Int. J. Mass Spec, 2005[DOI]
- Foote, C. S, "Photosensitizes oxidation and singlet oxygen: consquences in biological system Vol. 2", pp.85~133 (1976), Free radicals in biology, Academic Press
- Foote, C. S., "Definition of type and type photosensitized oxidation", Photochem. Photobiol, 54, pp.659~662 (1991)
- Hill, H. Z., "Melanin : a two edged sword?", Pigment. Cell. Res, 10, pp.158~161 (1997)
- Hosoi, J., "Regulation of melanin synthesis of B16 mouse melanoma cell by 1α,25- dihydroxyvitamin D3 and retinoic acid", Cancer Res, 45, pp.1474~1478 (1985)
- Ito, S., "Quantitative analysis of eumelanin and pheomelanin in humans, mice, and other animals: a comparative review", Pig. Cell Res, 16, pp.523~531 (2003)
- Kahl, R., "Toxicology of the synthetic antioxidants BHA and BHT in comparison with the natural antioxidant vitamin E", Z. Lebensm. Unters. Forsch, 196, pp.329~338 (1993)
- Kim, K. H., "Synthesis of novel kojic acid perivative and its anti-pigmentation effect", J. Soc. Cos. Sci. Kor, 30, pp.409~414 (2004)
- Kochanek, K. S., "Photoaging of the skin from phenotype to mechanisms", Exp. Gerontol, 35, pp.307~316 (2000)
- Kochanek, K. S., "Photoaging of the connective tissue of skin: Its prevention and therapy, In: Antioxidant in disease mechanisms and therapy", Adv. Pharmacol, 38, pp.639~655 (1996)
- Kyrtopoulos, S., "A, N-nitroso compoud formation in human gastric juice", Cancer Surv, 8, pp.423~442 (1989)
- Ling, T. J., "Antiseptic activity and phenolic constituents of the aerial parts of Vitex negundo var. cannabifolia", Molecules, 15, pp.8469~8477 (2010)
- Magdalena, L. C., "Reactive oxygen species, cellular redox system, and apoptosis", Free Radic. Biol. Med, 48, pp.749~762 (2010)
- Marmol, V., "Tyrosinase and related proteins in memmalian pigmentation", FEBS Letters, 381, pp.165~168 (1996)
- Matsusukami, M, "Evaluation of antimelanogenic effect", Frangrance, 19, pp.14~19 (1995)
- Muramatsu, H., "Superoxide dismutase in SAS human tongue carcinoma cell line is a factor defining invasiveness and cell motility", Cancer Res, 55, pp.6210~6214 (1995)
- Park, I. H., "An antioxidant hispidin from the mycelial cultures of Phellinus linteus", Arch. Pharm. Res, 27, pp.615~618 (2004)
- Park, S. N., , pp.1989
- Park, S. N., "Skin aging and antioxidant", J. Soc. Cosmet. Sci. Kor, 23, pp.75~132 (1997)
- Park, S. N., "Antioxidant properties of baicalein, component from Scutellaria Baicalensis Georgi and its application to cosmetics (I)", J. Kor. Ind. Eng. Chem, 14, pp.657~665 (2003)
- 박수남, "O2으로 유도된 사람 적혈구의 광용혈에 있어서 대두의 아이소플라본인 제니스테인의 보호작용", 한국식품과학회지, 35(3), pp.510~518 (2003), 한국식품과학회
- 박수남, "상수리나무 잎 추출물의 항균 및 항산화 활성과 활성 물질 분리", 대한화장품학회지, 35(2), pp.159~169 (2009), 대한화장품학회
- Pereira, C. A. M., "Distinction of the C-glucosylflavone isomer paors orientin", isoorientin and vitexin, Phytochem. Anal, pp.2005, 16[DOI]
- Seiberg, M., "Keratinocyte-melanocyte interation during melanosome transfer", Pigment. Cell Res, 14, pp.236~242 (2001)
- Sharov, V. S., "Selective sensitization of chemiluminescence resulted from lipid and oxygen radical reactions", Free Radical Biology & Medicine, 7, pp.237~242 (1989)
- Sohala, R. S., "The redox stress hypothesis of aging", Free Radic. Biol. Med, 52, pp.539~555 (2012)
- Takashi, Y., "Studies on inhibition mechanism of autoxidation by tannins and flavonoids. V. radical- scavenging effects of tannins and related polyphenols on 1,1-diphenyl-2-picrylhydrazyl radical", Chem. Pharm. Bull, 37, pp.1919~1921 (1989)
- Tang, Y., "Chemical composition and the regional effect of Vitex negundo Linn. var. cannabifolia (Sieb.et Zucc.) Hand.-Mazz. essential oils and the regional effect on it", J. Chem. Soc. Pak, 33, pp.744~746 (2011)
- Tripath, Y. B., "Antioxidant properties of different fractions of Vitex negundo Linn", Food Chemistry, 100, pp.1170~1176 (2007)
- Yaar, M., "Photoageing : mechanism, prevention and therapy", Br. J. Dermatol, 157, pp.874~887 (2007)
- Yamakoshi, J., "Lightening effect on ultravioletinduced pigmentation of guinea pig skin by oral administration of a proanthocyanidin-rich extract from grape seeds", Pig. Cell Res, 16, pp.629~638 (2003)