Anti-Oxidant Property and Inhibition of Melanin Synthesis of Eight Plant Extracts
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초록
Plants extracts are good resources to find functional compounds for human health. The following eight plants were collected and total phenolic contents were determined. Acer psedo-siebolianum showed the highest phenolic contents, 16.4 mg/g, whereas Cercidiphyllum japonica showed the lowest contents, 1.9 mg/g. The DPPH free radical scavenging capacities of the plant extracts showed high activity in following order : Acer ginnala (21.3 μg/mL) > Cornus walteri (23.9 μg/mL) > Distylum racemosum (29.2 μg/mL) > Castanopsis cuspidata var. Thunbergii (31.7 μg/mL) > Acer psedo-siebolianum (34.6 μg/mL) > Thuijopsis dolabrata cv. Aurea (53.1μg/mL) > Cercidiphyllum Japonica (115.2 μg/mL). Also the mushroom tyrosinase inhibitory activities of total extracts were determined at different concentration. D. racemosum extract showed highest (49.1% at 1,000mg) in inhibitory activity than other seven extracts. The ethanol fraction (IC50 value: 118.1 μg/mL) from D. racemosum showed more inhibitory activity than ethyl acetate fraction (IC50 value: 203 μg/mL). The ethanol fraction on showed no significant cytotoxicity in B16/F1 cells line up to 60 μg/mL. Over 80 μg/mL of ethanol fraction showed cytotoxicity in B16/F1 cells. The melanin contents of cells were significantly attenuated by ethanol fraction in a dose-dependent manner. The IC50 value of ethanol fraction was 75.4 μg/mL.
Abstract
Plants extracts are good resources to find functional compounds for human health. The following eight plants were collected and total phenolic contents were determined. Acer psedo-siebolianum showed the highest phenolic contents, 16.4 mg/g, whereas Cercidiphyllum japonica showed the lowest contents, 1.9 mg/g. The DPPH free radical scavenging capacities of the plant extracts showed high activity in following order : Acer ginnala (21.3 μg/mL) > Cornus walteri (23.9 μg/mL) > Distylum racemosum (29.2 μg/mL) > Castanopsis cuspidata var. Thunbergii (31.7 μg/mL) > Acer psedo-siebolianum (34.6 μg/mL) > Thuijopsis dolabrata cv. Aurea (53.1μg/mL) > Cercidiphyllum Japonica (115.2 μg/mL). Also the mushroom tyrosinase inhibitory activities of total extracts were determined at different concentration. D. racemosum extract showed highest (49.1% at 1,000mg) in inhibitory activity than other seven extracts. The ethanol fraction (IC50 value: 118.1 μg/mL) from D. racemosum showed more inhibitory activity than ethyl acetate fraction (IC50 value: 203 μg/mL). The ethanol fraction on showed no significant cytotoxicity in B16/F1 cells line up to 60 μg/mL. Over 80 μg/mL of ethanol fraction showed cytotoxicity in B16/F1 cells. The melanin contents of cells were significantly attenuated by ethanol fraction in a dose-dependent manner. The IC50 value of ethanol fraction was 75.4 μg/mL.
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