황칠나무 잎 추출물의 세포 항산화 활성과 미백활성 측정
Cellular Antioxidant Activity and Whitening Effects of Dendropanax morbifera Leaf Extracts.
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초록
본 연구에서는 황칠나무 잎 추출물의 HaCaT 세포에서의 항산화 활성과 B16F1 melanoma 세포에서의 미백활성을 측정하였다. HaCaT 세포를 이용한 실험에서, 황칠나무 잎 추출물의 에틸아세테이트 분획과 아글리콘 분획은 각각 50 및 25 μg/ml의 농도 이하에서 독성을 나타내지 않았다. UVB 800 mJ/cm2를 HaCaT 세포에 조사하였을 때, 황칠나무 잎추출물은 농도 의존적으로 자외선으로부터 세포를 보호하였다. 10 mM의 H2O2 및 4 μM의 rose bengal을 HaCaT 세포에 처리하였을 때, 에틸아세테이트 분획(6.25~50 μg/ml) 및 아글리콘 분획(6.25~25 μg/ml)은 우수한 세포 보호 효과를 나타내었다. 황칠나무 잎 추출물의 B16F1 melanoma 세포에서의 미백활성을 측정한 결과, 미백제로 알려진 알부틴 보다 더 우수한 멜라닌 합성 저해 활성을 보였다. 이상의 결과 들은 황칠나무 잎 추출물이 ROS에 대항하여 세포를 보호함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 세포보호제 및 천연항산화제로서 작용할 수 있음을 가르키며, α- MSH로 유도된 멜라닌 생합성 저해 효과로부터 황칠나무 잎추출물이 새로운 미백 화장품의 원료로써 이용 가능함을 알 수 있다.
Abstract
In this study, we investigated the antioxidant activities on HaCaT and the whitening effects on B16F1 melanoma cells of Dendropanax morbifera leaf extract. In an antioxidative activity assay using HaCaT cells, the ethyl acetate (50 μg/ml) and aglycone fractions (25 μg/ml) of the D. morbifera leaf extract didn't exhibit any characteristics of cytotoxicity. When HaCaT cells were exposed to a single large dose (800 mJ/cm2) of UVB, the extracts protected the cells against UVB radiation. When HaCaT cells were treated with 10 mM H2O2 and 4 μM rose bengal, the ethyl acetate (6.25~50 μg/ml) and aglycone (6.25~25 μg/ml) fractions protected the cells against oxidative damage in a concentration dependent manner. When the whitening effects of D. morbifera leaf extract were tested in melanoma B16/F1 cells treated with the a-melanocyte stimulating hormone (α-MSH), the extracts inhibited α-MSH-stimulated intra/extracellular melanogenesis in a concentration dependent manner. The inhibitory effects of the ethyl acetate and aglycone fractions of D. morbifera leaf extract were 21% and 44% at 25 μg/ml, respectively. Both are more effective than arbutin (15% at 25 μg/ml) which is known as a whitening agent. These results indicate that fractions of the D. morbifera leaf can function as cell protectants and natural antioxidants in biological systems, particularly skins exposed to UV radiation by quenching and/or scavenging 1O2 and other ROS, and protecting cells against ROS. In addition,fractions of the D. morbifera leaf can be applied to new whitening cosmetics because of their inhibitory effects on α-MSH stimulated melanogenesis in B16F1 melanoma cells.
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