Properties of Antimicrobial Substances Produced by Bacillus amyloliquefaciens CJW15 and Bacillus amyloliquefaciens SSD8
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Two Bacillus strains, CJW15 and SSD8, with strong antibacterial activities were isolated from cheonggukjang. Both were identified as B. amyloliquefaciens strains after gene sequencing of rRNA and recA. CJW15 strongly inhibited the growth of B. cereus (ATCC14579), Listeria monocytogenes (ATCC19111), and Lactococcus lactis (ATCC11454). In comparison, SSD8 inhibited the growth of B. cereus (ATCC14579) and Enterococcus faecium (ATCC19953). The antibacterial activities of the two strains were not affected when exposed to a temperature of 100oC for 15 min and were quite stable in acidic (pH 3) and alkaline (pH 12) pH conditions. Enzymatic treatments (trypsin, pepsin, proteinase K, and protease) had no effect on the activity of CJW15, but reduced the activity of SSD8 by half. Both isolates possess genes involved in the synthesis of lipopeptides (e.g. surfactin, fengycin, iturin, and iturin A), and genes encoding subtilin, a bacteriocin. Moreover, both isolates have fibrinolytic activities as well.
Abstract
Two Bacillus strains, CJW15 and SSD8, with strong antibacterial activities were isolated from cheonggukjang. Both were identified as B. amyloliquefaciens strains after gene sequencing of rRNA and recA. CJW15 strongly inhibited the growth of B. cereus (ATCC14579), Listeria monocytogenes (ATCC19111), and Lactococcus lactis (ATCC11454). In comparison, SSD8 inhibited the growth of B. cereus (ATCC14579) and Enterococcus faecium (ATCC19953). The antibacterial activities of the two strains were not affected when exposed to a temperature of 100oC for 15 min and were quite stable in acidic (pH 3) and alkaline (pH 12) pH conditions. Enzymatic treatments (trypsin, pepsin, proteinase K, and protease) had no effect on the activity of CJW15, but reduced the activity of SSD8 by half. Both isolates possess genes involved in the synthesis of lipopeptides (e.g. surfactin, fengycin, iturin, and iturin A), and genes encoding subtilin, a bacteriocin. Moreover, both isolates have fibrinolytic activities as well.
참고문헌 (17)
- Chung S, "Isolation and partial characterization of Bacillus subtilis ME488 for suppression of soilborne pathogens of cucumber and pepper", Appl. Microbiol. Biotechnol, 80, pp.115~123 (2008)
- Elsser-Gravesen D, "Biopreservatives", Adv. Biochem. Eng. Biotechnol, 143, pp.29~49 (2014)
- Gálvez A, "Application of bacteriocins in the control of foodborne pathogenic and spoilage bacteria", Crit. Rev. Biotechnol, 28, pp.125~152 (2008)
- Granum PE, "Bacillus cereus and its food poisoning toxins", FEMS Microbiol. Lett, 157, pp.223~228 (1997)
- Hoover DG, "Bacteriocins of Lactic Acid Bacteria", pp.23~39 (1993), Academic Press
- 정선주, "Cloning of Fibrinolytic Enzyme Gene from Bacillus subtilis Isolated from Cheonggukjang and Its Expression in Protease-deficient Bacillus subtilis Strains", Journal of Microbiology and Biotechnology, 17(6), pp.1018~1023 (2007), 한국미생물·생명공학회
- 정선주, "Characterization of AprE176, a Fibrinolytic Enzyme from Bacillus subtilis HK176", Journal of Microbiology and Biotechnology, 25(1), pp.89~97 (2015), 한국미생물·생명공학회[DOI]
- Kindoli, Salum, "Properties of Bac W42, a Bacteriocin Produced by Bacillus subtilis W42 Isolated from Cheonggukjang", Journal of Microbiology and Biotechnology, 22(8), pp.1092~1100 (2012), 한국미생물·생명공학회[DOI]
- Kwon GH, "Development of a RAPD-PCR method for identification of Bacillus species isolated from Chunggukjang", Int. J. Food Microbiol, 129, pp.282~287 (2009)
- Hwang A Lee, "Isolation of Bacillus amyloliquefaciens Strains with Antifungal Activities from Meju", Preventive Nutrition and Food Science, 17(1), pp.64~70 (2012), 한국식품영양과학회[DOI]
- Xiaoming Liu, "Properties of a Bacteriocin Produced by Bacillus subtilis EMD4 Isolated from Ganjang (Soy Sauce)", Journal of Microbiology and Biotechnology, 25(9), pp.1493~1501 (2015), 한국미생물·생명공학회[DOI]
- Sarangi NP, "Identification of antifungal antibiotics of Bacillus species isolated from different microhabitats using polymerase chain reaction and MALDI-TOF mass spectrometry", Can. J. Microbiol, 55, pp.1021~1032 (2009)
- Schägger H, "Tricine-sodium dodecyl sulphate polyacylamide gel electrophoresis for the separation of protein in the range from 1 to 100 kDa", Anal. Biochem, 166, pp.368~379 (1987)
- Settanni L, "Application of bacteriocins in vegetable food biopreservation", Int. J. Food Microbiol, 121, pp.123~138 (2008)
- Stein T, "Bacillus subtilis antibiotics: structures, syntheses and specific functions", Mol. Microbiol, 56, pp.845~857 (2005)
- Sutyak KE, "Isolation of the Bacillus subtilis antimicrobial peptide subtilosin from the dairy product-derived Bacillus amyloliquefaciens", J. Appl. Microbiol, 104, pp.1067~1074 (2008)
- Tapi A, "New approach for the detection of non-ribosomal peptide synthetase genes in Bacillus strains by polymerase chain reaction", Appl. Microbiol. Biotechnol, 85, pp.1521~1531 (2010)