In vitro 실험을 통한 벼세포 추출물의 창상 치유 효능 평가
Evaluation of the Wound-healing Activity of Rice Cell Extracts in Vitro
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
In the present study, we evaluated the in vitro wound-healing properties of two types of rice cell extracts (RCEs; prepared using ethanol and pressurized hot water extraction methods), using human dermal fibroblasts and keratinocytes. The effects of the RCEs (at 25–100 μg/ml) on cytotoxicity and cell migration were assessed. Both RCEs were not cytotoxic to the two cell types, instead increasing their proliferation by up to 25% in a dose-dependent manner compared with the controls. Furthermore, both RCEs significantly enhanced the migratory ability of the two cell types (fibroblast, 230–450%; keratinocyte, 170–350%). Additionally, we examined the effect of the RCEs on type I collagen synthesis, which is important in the wound reconstruction process. The RCEs significantly increased collagen type I mRNA and protein levels to a degree comparable to that induced by vitamin C. These results suggest the RCEs to be candidate materials for use in promoting wound healing, through their actions of increasing cell migration and accelerating wound re-epithelialization.
Abstract
In the present study, we evaluated the in vitro wound-healing properties of two types of rice cell extracts (RCEs; prepared using ethanol and pressurized hot water extraction methods), using human dermal fibroblasts and keratinocytes. The effects of the RCEs (at 25–100 μg/ml) on cytotoxicity and cell migration were assessed. Both RCEs were not cytotoxic to the two cell types, instead increasing their proliferation by up to 25% in a dose-dependent manner compared with the controls. Furthermore, both RCEs significantly enhanced the migratory ability of the two cell types (fibroblast, 230–450%; keratinocyte, 170–350%). Additionally, we examined the effect of the RCEs on type I collagen synthesis, which is important in the wound reconstruction process. The RCEs significantly increased collagen type I mRNA and protein levels to a degree comparable to that induced by vitamin C. These results suggest the RCEs to be candidate materials for use in promoting wound healing, through their actions of increasing cell migration and accelerating wound re-epithelialization.
참고문헌 (35)
- Agnihotri S, "An overview on antiinflammatory properties and chemo-profiles of plants used in traditional medicine", Indian J. Nat. Prod. Resour, 1, pp.150~167 (2010)
- Atiyeh BS, "An update on management of acute and chronic open wounds: the importance of moist environment in optimal wound healing", Med. Chem. Rev, 1, pp.111~121 (2004)
- Bajaj P, "3D biofabrication strategies for tissue engineering and regenerative medicine", Annu. Rev. Biomed. Eng, 16, pp.247~276 (2014)
- Boukamp P, "Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line", J. Cell Biol, 106, pp.761~771 (1988)
- Broughton II G, "The basic science of wound healing", Plast. Reconstr. Surg, 117, pp.12S~34S (2006)
- Caley MP, "Metalloproteinases and wound healing", Adv. Wound Care, 4, pp.225~234 (2015)
- 천수환, "Enhanced Delivery of siRNA Complexes by Sonoporation in Transgenic Rice Cell Suspension Cultures", Journal of Microbiology and Biotechnology, 19(8), pp.781~786 (2009), 한국미생물·생명공학회
- 최재철, "Promotion Effects of Ultra-High Molecular Weight Poly-γ-Glutamic Acid on Wound Healing", Journal of Microbiology and Biotechnology, 25(6), pp.941~945 (2015), 한국미생물·생명공학회[DOI]
- Gurtner GC, "Wound repair and regeneration", Nature, 453, pp.314~321 (2008)
- Havsteen BH, "The biochemistry and medical significance of the flavonoids", Pharmacol. Ther, 96, pp.67~202 (2002)
- Huang C, "Are keloid and hypertrophic scar different forms of the same disorder? A fibroproliferative skin disorder hypothesis based on keloid findings", Int. Wound J, 11, pp.517~522 (2014)
- 김애향, "A Mixture of Ethanol Extracts of Persimmon Leaf and Citrus junos Sieb Improves Blood Coagulation Parameters and Ameliorates Lipid Metabolism Disturbances Caused by Diet-Induced Obesity in C57BL", 6J Mice, 2016, pp.26(2), 295~308[DOI]
- 김동균, "Construction of Chimeric Human Epidermal Growth Factor Containing Short Collagen-Binding Domain Moieties for Use as a Wound Tissue Healing Agent", Journal of Microbiology and Biotechnology, 25(1), pp.119~126 (2015), 한국미생물·생명공학회[DOI]
- Kim KO, "Wound healing properties of a 3-D scaffold comprising soluble silkworm gland hydrolysate and human collagen", Colloids Surf. B, 116, pp.318~326 (2014)
- 김지훈, "A Composite Dermal Filler Comprising Cross-Linked Hyaluronic Acid and Human Collagen for Tissue Reconstruction", Journal of Microbiology and Biotechnology, 25(3), pp.399~406 (2015), 한국미생물·생명공학회[DOI]
- Kwon JY, "Bioreactor engineering using disposable technology for enhanced production of hCTLA4Ig in transgenic rice cell cultures", Biotechnol. Bioeng, 110, pp.2412~2424 (2013)
- Lai PKK, "Anti‐inflammatory activities of an active fraction isolated from the root of Astragalus membranaceus in RAW 264.7 Macrophages", Phytother. Res, 28, pp.395~404 (2014)
- Lam KS, "New aspects of natural products in drug discovery", Trends Microbiol, 15, pp.279~289 (2007)
- 이기만, "Chitin from the Extract of Cuttlebone Induces Acute Inflammation and Enhances MMP1 Expression", Biomolecules & Therapeutics, 21(3), pp.246~250 (2013), 한국응용약물학회
- Lu Y, "Polyphenolics of Salvia-a review", Phytochemistry, 59, pp.117~140 (2002)
- Maquart F-X, "Stimulation of collagen synthesis in fibroblast cultures by a triterpene extracted from Centella asiatica", Connect. Tissue Res, 24, pp.107~120 (1990)
- Martin P, "Wound healing-aiming for perfect skin regeneration", Science, 276, pp.75~81 (1997)
- Meda A, "Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity", Food Chem, 91, pp.571~577 (2005)
- Middleton Jr E, "Flavonoids in the Living System", pp.175~182 (1998), Springer US
- Miean KH, "Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants", J. Agric. Food Chem, 49, pp.3106~3112 (2001)
- Niiyama H, "Development of novel wound dressing composed of hyaluronic acid and collagen sponge containing epidermal growth factor and vitamin C derivative", J. Artif. Organs, 17, pp.81~87 (2014)
- Okarter N, "Health benefits of whole grain phytochemicals", Crit. Rev. Food Sci. Nutr, 50, pp.193~208 (2010)
- Reuter J, "Botanical Medicine. From Bench to Beside", pp.33~65 (2009), Mary Ann Liebert Inc
- Robles DT, "Abnormal wound healing: keloids", Clin. Dermatol, 25, pp.26~32 (2007)
- Shi G, "Evaluation of the wound-healing activity and anti-inflammatory activity of aqueous extracts from Acorus calamus L", Pak. J. Pharm. Sci, 27, pp.91~95 (2014)
- 신소림, "추출방법 및 용매에 따른 청나래고사리의 항산화 활성", 생명과학회지, 21(1), pp.56~61 (2011), 한국생명과학회
- Suguna L, "Effects of Centella asiatica extract on dermal wound healing in rats", Indian J. Exp. Biol, 34, pp.1208~1211 (1996)
- Werner S, "Regulation of wound healing by growth factors and cytokines", Physiol. Rev, 83, pp.835~870 (2003)
- Yanagawa H, "Chemical components of callus tissues of rice", Phytochemistry, 11, pp.1893~1897 (1972)
- Xiu J, "Effect of asiaticoside on hypertrophic scar in the rabbit ear model", J. Cutan. Pathol, 36, pp.234~239 (2009)