솔잎 추출물이 진피섬유아세포에서 콜라겐 발현 및 생성에 미치는 영향
The Effects of Pini Folium Extract on Collagen Expression and Synthesis in Normal Human Dermal Fibroblasts
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By using Pini Folium of Pinus densiflora Seib. et Zucc. that naturally grow in Gangwon-do area, cytotoxicity resistance effect against NHDF were examined and a study on the mRNA expression of COL1A1 and generation effect of pro-collagen 1 was conducted. After extracting Pini Folium with 80% ethanol and hot water respectively and they were referred to as PEF and PFW. The cytotoxicity resistance effect of pine needles was examined by conducting MTT assay, TNF-α, and H2O2 test regarding NHDF. The amount of COL1A1expression using qRT-PCR and the generation of pro-collagen 1 using Western blot were examined. As a result of the experiment, In MTT assay, PFE, PFW had no cytotoxicity against the hypodermal cell. As a result of cell death resistance effect on TNF-α, PFE displayed a significant result in the concentration of 10 μg/mL and PFW displayed non signifiant result. In the effects on cell death resistance of H2O2, PFE and PFW could be confirmed to have significant results. In regards to the measurement of mRNA expression of COL1A1 , PFE displayed non-significant difference, PFW displayed significant difference. In regards to the generation of procollagen 1, the protein was generated for both PFE, PFW. In conclusion, PFE and PFW of Pini Folium extract can be applied to skin beauty practically and considered as the results to suggest the anti-aging possibility in aging skin in regards to mRNA expression of COL1A1 against NHDF and generation of pro-collagen 1.
Abstract
By using Pini Folium of Pinus densiflora Seib. et Zucc. that naturally grow in Gangwon-do area, cytotoxicity resistance effect against NHDF were examined and a study on the mRNA expression of COL1A1 and generation effect of pro-collagen 1 was conducted. After extracting Pini Folium with 80% ethanol and hot water respectively and they were referred to as PEF and PFW. The cytotoxicity resistance effect of pine needles was examined by conducting MTT assay, TNF-α, and H2O2 test regarding NHDF. The amount of COL1A1expression using qRT-PCR and the generation of pro-collagen 1 using Western blot were examined. As a result of the experiment, In MTT assay, PFE, PFW had no cytotoxicity against the hypodermal cell. As a result of cell death resistance effect on TNF-α, PFE displayed a significant result in the concentration of 10 μg/mL and PFW displayed non signifiant result. In the effects on cell death resistance of H2O2, PFE and PFW could be confirmed to have significant results. In regards to the measurement of mRNA expression of COL1A1 , PFE displayed non-significant difference, PFW displayed significant difference. In regards to the generation of procollagen 1, the protein was generated for both PFE, PFW. In conclusion, PFE and PFW of Pini Folium extract can be applied to skin beauty practically and considered as the results to suggest the anti-aging possibility in aging skin in regards to mRNA expression of COL1A1 against NHDF and generation of pro-collagen 1.
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