數種 한약재의 콜라겐 생합성 효과에 대한 실험적 연구
Experimental study about the effect of several herbs on collagen synthesis
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Background & Objective : This study was performed to find a new anti-aging, anti-wrinkle agent. Ten kinds of hers were evaluated by proliferation, cytotoxicity, collagen synthesis tests in cultured human dermal fibroblast. Methods : MTT assay was carried out to check cell proliferation, cytotoxic effect of each herbal medicine. Collagen synthesis was investigated by the amount of propeptide using procollagen type-Ⅰ C peptide(PIP)ELISA kit(Takara). Results and Conclusions : There are no cytotoxicity in all group herbs by MTT assay. Collagen synthetic ability was greatest in LYCII FRUCTUS(LF) and was excellent in order of Rehmanniae Radix Preparat(RRP) >Nelumbinis Semen(NS) > Asparai Radix(AR) > Schizandrae Frucus(SF).
Abstract
Background & Objective : This study was performed to find a new anti-aging, anti-wrinkle agent. Ten kinds of hers were evaluated by proliferation, cytotoxicity, collagen synthesis tests in cultured human dermal fibroblast. Methods : MTT assay was carried out to check cell proliferation, cytotoxic effect of each herbal medicine. Collagen synthesis was investigated by the amount of propeptide using procollagen type-Ⅰ C peptide(PIP)ELISA kit(Takara). Results and Conclusions : There are no cytotoxicity in all group herbs by MTT assay. Collagen synthetic ability was greatest in LYCII FRUCTUS(LF) and was excellent in order of Rehmanniae Radix Preparat(RRP) >Nelumbinis Semen(NS) > Asparai Radix(AR) > Schizandrae Frucus(SF).
참고문헌 (26)
- Branchet MC, "Skin thickness changes in normal aging skin", Gerontology, 36, pp.28~35 (1990)
- Green LJ, "Photoaging and the skin: The effects of tretinoin", Dermatol Clin, 11, pp.97~105 (1993)
- Yaar M, "Aging versus photoaging: Postulated Mechanisms and effectors", J Invest Dermtol Symp Proc, 3, pp.47~51 (1998)
- Jerome S. P, "Idedntification of Collagen Fibrils in scleroderma skin", J. Invest. Dermatol, 90(1), pp.48~54 (1998)
- 성은진, "주름 치료의 한의학적 고찰", 한방안이비인후피부과학회지, 21(3), pp.184~199 (2008), 대한한방안이비인후피부과학회
- Parfitt AM, "Procollagen type Ⅰ carboxy-terminl extension peptide in serum as a marker of collagen biosynthesis in bone. Correlation with iliac formation rates and compasison with total alkaline phosphatase", J. Bone Miner Res, 2, pp.427~436 (1987)
- Loosdrecht AA, "Cell mediated cytotoxicity against U937 cells by human monocytes and macrophages in a modified colorimetric MTT assay. A methodological study", J Immunol Method, 141, pp.15~22 (1991)
- Wilhelm KP, "Skin aging. Effect on transepidermal water loss, stratum corneum hydration, skin surface pH, and casual sebum content", Arch Dermatol, 127, pp.1806~1806 (1991)
- Gilchrest BA, "Skin aging and photoaging:an overview", J Am Acad Dermatol, 21, pp.610~613 (1989)
- Bernstein EF, "Enhanced elastin and fibrillin gene expression in chronically photodamaged skin", J Invest Dermatol, 103(2), pp.182~186 (1994)
- Chiba K, "Characteristics of skin wrinkling and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin", Skin Pharmacology and Applied Skin Physiology, 16, pp.242~251 (2003)
- 朴贊國, "黃帝內經素問注釋", pp.7~97 (2005), 集文堂
- Burgeson RE, "Collagen types : Molecular structure and tissue distribution", Clin Orthop, 282, pp.250~272 (1992)
- Ihn, H, "Scleroderma, fibroblast, signaling and excessive extracellular matrix", Curr Rheumatol, 7, pp.156~162 (2005)
- Wulf H. C, "Skin aging and natural photoprotection", Micron, 35, pp.85~191 (2004)
- 대한피부과학회 교과서 편찬위원회, "피부과학", pp.17~18 (1994), 麗文閣
- Giacomoni PU, "Factors of skin aging share common mechanisms", Biogerontology, 2(4), pp.210~229 (2001)
- Parfitt AM, "Procollagen type Ⅰ carboxyterminal extention peptide in serum as a marker of collagen biosynthesis in bone. Correlation with iliac bone formation rates and comparison with total alkaline phosphatase", J. Bone Miner Res, 2, pp.427~436 (1987)
- Bonte F, "Influence of asiatic acid, madecassic acid, and aisiaticoside on human collagen Ⅰ synthesis", Planta Med, 60(2), pp.133~135 (1994)
- 김나미, "홍삼성분이 섬유아세포의 콜라겐 생합성과 MMP-I 활성에 미치는 영향", Journal of Ginseng Research, 31(2), pp.86~92 (2007), 고려인삼학회
- 정택규, "황기추출물의 보습 및 항산화 효과", 대한화장품학회지, 32(3), pp.193~200 (2006)
- 황성주, "유백피 추출물을 함유한 하이드로갤 패치의 주름 억제 효과", Journal of Pharmaceutical Investigation, 34(3), pp.193~199 (2004), 한국약제학회
- 진무현, "백지의 콜라겐 생성 촉진 물질", 생약학회지, 35(4), pp.315~319 (2004), 한국생약학회
- 구본석, "사상자(Torilis Fructus)가 섬유아세포의 Procollagen 생합성과Matrix metalloproteinase-I(MMP-1)의 활성에 미치는 영향", 생약학회지, 38(4), pp.349~353 (2007), 한국생약학회
- 강성례, "삼백초 열수추출물의 피부주름 억제효과", 한국미용학회지, 15(4), pp.1389~1398 (2009), 한국미용학회
- Ukeda H, "Spectrophotometric assay for superoxide dismutase based on tetrazolium salt 3'-1-(phenylamino)-carbonyl-3, 4-tetrazolium-bis (4-methoxy-6-nitro benzensulfonic acid hydrate reduction by xanthine-xanthine oxidase)", Anal Biochem, 5, pp.206~210 (1997)