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Salvia microphylla 추출물이 각질형성세포의 피부상처 및 피부장벽 기능에 미치는 영향

Effects of Salvia microphylla Extract on the Skin Wound and Barrier Function in Human Keratinocytes

최인호|조현미
한국피부과학연구원 (Korea Institute of Dermatological Sciences)|2024.12|아시안뷰티화장품학술지 (Asian Journal of Beauty and Cosmetology)|제22권 제4호|pp. 619-630

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키워드

각질형성세포재상피화Salvia microphylla피부장벽상처치유角质形成细胞，上皮再生，小叶鼠尾草，皮肤屏障，伤口愈合
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초록

목적: Salvia microphylla (SM)은 항돌연변이성, 리파아제 억제 및 살충 효과를 포함한 다양한 생물학적 활성이 연구 보고되었으나 SM에 대한 피부재생 또는 상처치유와 같은 인간 피부 생리 활성에 미치는 연구보고는 미비하다. 본 연구는 피부 각질형성세포에서 SMW의 상처치유 및 피부장벽 개선 효과를 연구하였다. 방법: SMW의 항산화 활성은 DPPH 및 ABTS radical 소거능 방법을 이용하여 측정하였다. 증식은 MTT 분석을 사용하여 측정하였으며, 이동은 스크래치 방법을 사용하여 측정하였다. 증식과 이동 관련 신호전달체계 확인을 위하여 Weston blot을 통해 확인하였다. Type Ⅰ collagen 합성 및 MMP-1 생성은 ELISA에 의해 확인하였다. 또한, filaggrin, involucrin 및 loricrin의 mRNA 발현은 RT-qPCR로 측정하였다. 결과: SMW의 DPPH 및 ABTS radical 소거 활성은 농도 의존적으로 증가하였다. SMW은 각질형성세포(HaCaTs)의 증식 및 이동을 유도하였으며, 특히 400 μg/mL에서 133.03%의 증식과 213.43%의 이동 유도 활성을 나타내었다. 또한, SMW는 각질형성세포에서 ERK 1/2, p38 MAPK 및 AKT의 인산화에 대한 효과를 나타내었으며, Type I Collagen 합성을 자극하고 MMP-1 생성을 감소시켰다. 피부장벽의 구조와 기능에 중요한 역할을 하는 단백질인 filaggrin 및 involucrin의 mRNA 발현이 유의적으로 증가하는 것을 확인하였다. 결론: 이러한 결과들은 SMW가 정상적인 각질형성세포의 재상피화 과정과 상처치유 및 피부장벽 개선에 긍정적인 영향을 미칠 수 있음을 보여줍니다. 향후 SMW는 화장품산업에서 피부재생 및 피부장벽 개선 소재로서 응용가능성을 검증하였다.

Abstract

Purpose: Salvia microphylla (SM) has variety bioactivities including antimutagenic, lipase-inhibitory and insecticidal effects. However, its biological activities on skin remain unclear. In this study, we examined the effect of SM water extract (SMW) of SM on skin wound healing- and skin barrier-linked responses in human keratinocytes (HaCaT cells). Methods: The antioxidant activities were measured using 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Proliferation was confirmed by MTT assay, and migration was measured using a scratch wound healing assay. In order to confirm the proliferation and migration-related signal pathway, it was confirmed by Western blot. Type Ⅰ collagen synthesis and MMP-1 production were confirmed by enzyme-linked immunosorbent assay (ELISA). In addition, the expression of filaggrin, involucurin, and loricin were measured by RT-qPCR. Results: DPPH and ABTS radical scavenging activity of SMW increased a concentration-dependent manner. SMW induced proliferation and migration of HaCaTs, especially at 400 μg/mL(proliferation-inducing activity=133.03%; migration-inducing activity=213.43%). Furthermore, SMW significantly enhanced the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2, p38 MAPK and serine/threonine-specific protein kinase (AKT) in HaCaTs, stimulated Type I collagen synthesis and reduced MMP-1 production. It was confirmed that mRNA expression of filaggrin and involucrin, proteins that play important roles in the structure and function of the skin barrier, was significantly increased. Conclusion: These results demonstrate that SMW has skin regeneration and wound healing activity and skin barrier improvement in HaCaTs. In the future, SMW was verified for its applicability as a material for skin regeneration and skin barrier improvement material in the cosmetics industry.

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