본문 바로가기
KERD
논문KCI 등재

Phytotherapy of experimentally induced gill inflammation with Aeromonas hydrophila infection in goldfish, Carassius auratus

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Goldfish, Carassius auratus (wt 13 g) was intramuscularly infected with Aeromonas hydrophila (4.3x106 cfu / ml). Infected gills showed edematous lamellae with bacterial invasion into the capillaries and gill congestion on 12th day. By 24th day post-infection, histological analysis revealed irregular aggregates of macrophages in gill lamellae, large amount of mucus cells, gill lamellae edematous with bacterial invasion into capillaries, gill congestion and damaged gill epithelium with hyperplasia. Inflammation of the gill filament and hemorrhage globe was associated with the development of severe necrosis on the 36th day in the infected fishes. In infected and herbal treated fish the regenerative responses like fibrosis and infiltration of the leucocytes (neutrophils and monocytes) occurred on 12th day; moderate hypertrophy in the gills was noticed on the 36th day. These results suggest that phytotherapy ensures better protection and regenerative response against A. hydrophila infection in goldfish, C. auratus.

Abstract

Goldfish, Carassius auratus (wt 13 g) was intramuscularly infected with Aeromonas hydrophila (4.3x106 cfu / ml). Infected gills showed edematous lamellae with bacterial invasion into the capillaries and gill congestion on 12th day. By 24th day post-infection, histological analysis revealed irregular aggregates of macrophages in gill lamellae, large amount of mucus cells, gill lamellae edematous with bacterial invasion into capillaries, gill congestion and damaged gill epithelium with hyperplasia. Inflammation of the gill filament and hemorrhage globe was associated with the development of severe necrosis on the 36th day in the infected fishes. In infected and herbal treated fish the regenerative responses like fibrosis and infiltration of the leucocytes (neutrophils and monocytes) occurred on 12th day; moderate hypertrophy in the gills was noticed on the 36th day. These results suggest that phytotherapy ensures better protection and regenerative response against A. hydrophila infection in goldfish, C. auratus.

참고문헌 (76)

  1. Abutbul, S., "Use of Rosmarinus officinalis as a treatment against Streptococcus iniae in tilapia (Oreochromis sp.)", Aquaculture, 238, pp.97~105 (2004)
  2. Adams, M.B., "Amoebic gill disease: sequential pathology in cultured Atlantic salmon, Salmo salar L.", J. Fish Dis, 26, pp.601~614 (2003)
  3. Adams, M.B., "Distribution and structure of lesions in the gills of Atlantic salmon, Salmo salar L., affected with amoebic gill disease", J. Fish Dis, 24, pp.535~542 (2001)
  4. Angka, S.L., "The pathology of the walking catfish, Clarias batrachus(L.), infected intraperitoneally with Aeromonas hydrophila", Asian Fish. Sci, 3, pp.343~351 (1990)
  5. Anto, R.J., "Antiinflammatory activity of natural and synthetic curcuminoids", Pharm. Pharmacol. Commun, 4, pp.103~105 (1998)
  6. Bell, S.C., "Immunochemical detection and characterization of pregnancy-associated endometrial α1-and α2-globulins secreted by human endometrium and decidua", J. Reprod. Fertil, 74, pp.261~270 (1985)
  7. Bhadauria, M., "Effect of propriety herbal formulation against chronic carbon tetrachloride induced hepatotoxicity", Indian J. Exp. Biol, 40, pp.1254~1259 (2002)
  8. Bouic, P.J.D., "Beta-sitosterol and Beta-sitosterol glucoside stimulate human peripheral blood lymphocyte proliferation: implications for their use as an immunomodulatory vitamin combination", Int. J. Immunopharmacol, 18, pp.693~700 (1996)
  9. Branson, E.J., "Description of a new disease condition occurring in farmed coho salmon, Oncorhynchus kisutch (Walbaum), in South America", J. Fish Dis, 14, pp.147~156 (1991)
  10. Campbell, R.E., "The use of natural products in the treatment of EUS (Epizootic Ulcerative Syndrome)", Proceedings of the International Symposium on Aquatic Animal Health. Baltimore (1998)
  11. Candan, A.A., "A study on the histopathology of Aeromonas hydrophila infectious of rainbow trout (Salmo gairdneri R.) kept under experimental conditions and the effect of chloramphenicol", J. Aquat. Prod, 4, pp.5~20 (1990)
  12. Chopra, R.N., "Glossary of Indian medical plants. New Delhi" (1956), Council of Scientific and Industrial Research
  13. Citarasu, T., "Influence of the antibacterial herbs, Solanum trilobatum, Andrographis paniculata and Psoralea corylifolia on the survival, growth and bacterial load of Penaeus monodon post larvae", Aquacult. Int, 1, pp.583~595 (2003)
  14. Colorni, A., "Histopathology of sea bass (Dicentrarchus labrax) experimentally infected with Mycobacterium marinum and treated with streptomycin and garlic (Alium sativum) extract", Aquaculture, 160, pp.1~17 (1998)
  15. Crespo, S., "Utraltrastructural alterations of the dogfish (Scyliorhinus canicula) gill filament related to experimental aquatcz inc pollution Dis", Aquat.. Org, 1, pp.99~104 (1986)
  16. Dalmo, R., "Non-specific defence-mechanisum in fish, with particular reference to the reticuloendothelial system (RES)", J. Fish Dis, 20, pp.241~273 (1997)
  17. Davis, J.F., "Pathogenic bacteria associated with cultured American eels Anguilla rostrata Le Sueur", J. Fish Dis, 23, pp.557~564 (1983)
  18. Dey, R.K., "Preliminary studies to raise disease resistant seed (fry) of Indian major carp, Catla catla (Ham.) through herbal treatment of spawn", Fish Chimes, 23(5) (1995)
  19. Dey, R.K., "On the use of herbal materials for managing diseases and health conditions of fish during sustainable aquaculture practices", Abstract, National workshop on fish and shellfish health management (1997)
  20. Donta, S.T., "Cytotoxic activity of Aeromonas hydrophila", Infect. Immun, 21, pp.989~993 (1978)
  21. Edahiro, T., "Effect of Glycyrrhizine against Streptococcal infection of young yellowtail, Seriola quinqueradiata", Suisan Zoshoku, 38, pp.239~243 (1990)
  22. Ellis, A.E., "The anatomy and physiology of teleosts, Fish Pathology", pp.13~55 (1989), Bailliere Tindall
  23. Evans, M., "A Guide to Herbal Remedies", pp.9~12 (1994), Orient paper back, Delhi
  24. Fanta, E., "Histopathology of the fish Corydoras paleatus contaminated with sublethal levels of organophosphorus in water and food", Ecotoxicol. Environ. Safety, 54, pp.119~130 (2003)
  25. Fujiki, K., "Protective effect of sodium alginates against bacterial infection in common carp, Cyprinus carpio L.", J. Fish Dis, 17, pp.349~355 (1994)
  26. Gernhofer, M., "Ultrastructural biomarkers as tools to characterize the health status of fish in contaminated streams", J. Aquat. Ecosystem Stress and Recov, 8, pp.241~260 (2001)
  27. Govindan, S., "Further studies on the clinical efficacy of Solanum xanthocarpum and Solanum trilobatum in bronchial asthma", Phytothera. Res, 18, pp.805~809 (2004)
  28. Grizzle, J. M., "Histopathology of gill, liver and pancreas and serum enzyme levels of channel catfish infected with Aeromonas hydrophila complex", J. Aquat. Animal Health, 5, pp.36~50 (1993)
  29. Gupta, S.K., "Validation of traditional claim of Tulsi, Ocimum sanctumLinn as a medicinal plant", Indian J. Exp. Biol, 40, pp.765~773 (2002)
  30. Harikrishnan, R., "Herbal treatment for ulcerative disease induced by Aeromonas hydrophila in goldfish (Carassius auratus)", 박사, pp.2003
  31. Harikrishnan, R., "Modern trends in Aeromonas hydrophila disease management with fish", Rev. Fish. Sci, 13, pp.281~320 (2005)
  32. Harikrishnan, R., "Restorative effect of Azadirac hta indica aqueous leaf extract dip treatment on haematological parameter changes in Cyprinus carpio (L.) experimentally infected with Aphanomyces invadans fungus", J. Appl. Ichthyol, 21, pp.410~414 (2005)
  33. Harikrishnan, R., "Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromo nas hydrophila infection", Aquaculture, 221, pp.41~50 (2003)
  34. Hemapriya, V. S., "Immunostimulation by leaf extracts of a few south Indian medicinal plants in Oreochromis mossambicus (Peters)", Dev. Comp. Immunol, 21, pp.175~179 (1997)
  35. Hinton, D.E., "Liver structural alterations accompanying chronic toxicity in fishes: potentioal biomarkers of exposure", pp.51~65 (1990), Biomarkers of Environmental Contamination. Boca Raton, Lewis Publishers
  36. Hocutt, C.H., "Changes in gill morphology of Oreochromis rnossambicus subjected to heat stress", Environ. Biol. Fish, 14, pp.107~114 (1985)
  37. Huizinga, H.W., "Histopathology of red-sore disease (Aeromonas hydrophila) in naturally and experimentally infected largemouth bass Micropterus salmoides (Lacepeda)", J. Fish Dis, 2, pp.263~277 (1979)
  38. Ilori, M.O., "Antidiarrhoeal activities of Ocimum gratissimum (Lamiaceae)", J. Diarrhoeal Dis. Res, 14, pp.283~285 (1996)
  39. Iwalokun, B.A., "Epidemiology of shigellosis in Lagos, Nigeria: trends in antimicrobial resistance", J. Health Popul. Nutr, 19, pp.183~190 (2001)
  40. Janssen, D.B., "Cloning of 1, 2 - Dichloroethane Degradation genes of Xanthobacter autotrophicus GJ10 and Expression and Sequencing of the dhlA Gene", J. Bact, 171, pp.6791~6799 (1989)
  41. Joseph, S.W., "The Isolation, Identification, and Systematic of the motile Aeromonas species", Annual Rev. Fish Dis, 4, pp.31~343 (1994)
  42. Kongtorp, R.T., "Heart and skeletal muscle inflammation in Atlantic salmon, Salmo salar L.: a new infectious disease", J. Fish Dis, 27, pp.351~358 (2004)
  43. Kovacs-Gayer, E., "Fish, pathogens and environment In European polyculture", pp.219~232 (1984), Akademiai & Ado, Budapest
  44. Laglar, K.F., "Ichthyology" (1963), John Wiley and Sons Inc.
  45. Logambal, S.M., "Azadirachtin- An immunostimulant in Oreochromis mossa mbicus (Peters)", Abstract, National workshop on fish and shellfish health management, CIFA, Bhubaneswar (1994)
  46. Mitra, C.R., Garg, H.S. and Pandey, G.N., "Constituents of Azadirachta indica 3. identification of nimbidic acid and nimbidinin from Azadirachta indica", Phytochemistry, 10, pp.857~864 (1971)
  47. Miyazaki, T., "Histopathology of viremia-associated ana-aki-byo in combination with Aeromonas hydrophila in color carp Cyprinus carpio in Japan", Dis. Aquat. Org, 44, pp.100~120 (2001)
  48. Miyazaki, M., "Forms and morphs of aphids, Aphids: Their biology, natural enemies and control", pp.27~50 (1987), Elsevier Science Publisher
  49. Morrison, R.N., "The histopathological effects of a levamisoleadjuvanted Vibrio anguillarum vaccine on Atlantic salmon (Salmo salar L.)", Aquaculture, 195, pp.23~33 (2001)
  50. Nadkarni, K.M., "Indian Materia Medica", pp.1260~1262 (1976), Popular Prakashan, Bombay
  51. Nakamura, C.V., "Antibacterial activity of Ocimum gratissmum L. essential oil", Mem Inst Oswaldo Cruz Rio deJaneiro, 94, pp.675~678 (1999)
  52. Ortuno, J., "High dietary intake of α-tocopherol acetate enhances the non-specific immune response of gilthead seabream (Sparus auratus L.)", Fish Shellfish Immunol, 10, pp.293~307 (2000)
  53. Pazhanisamy, K., "Studies on the impact of arsenic trioxide on a freshwater fish, Labeo rohita (Hamilton)", 박사, pp.2002
  54. Peters, G., "Environmentalinduced gill disease of cultured rainbow trout (Salmo gairdneri)", Aquaculture, 38, pp.105~126 (1984)
  55. Peters, G., "Gill structure and blood electrolyte levels of European eels under stress, Fish and shellfish pathology", pp.183~198 (1985), Academic Press
  56. Rao, Y.V., "Effect of Achyranthes aspera on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila", Fish Shellfish Immunol, 20, pp.263~273 (2006)
  57. Rath, R.K., "Freshwater Aquaculture", pp.282~312 (1993), Scientific Publishers
  58. Ray, A., "Modulation of humoral and cell mediated immune responses by Azadirachta indica (Neem) in mice", Indian J. Exp. Biol, 34, pp.698~701 (1996)
  59. Rojanapo, W., "Screening of antioxidants from some Thai vegetables and herbs", J. Sci. Thailand, 11, pp.177~188 (1985)
  60. Sadekar, R.D., "Immunopotentiating effects of Azadirachta indica (Neem) dry leaves powder in broilers, naturally infected with IBD virus", Indian J. Exp. Biol, 36, pp.1151~1153 (1998)
  61. Schwaiger, J., "The use of histopatological indicators to evaluate contaminant- related stress in fish", J. Aquat. Ecossystem Stress and Recovery, 6, pp.75~86 (1997)
  62. Selvam, R., "The anti-oxidant activity of turmeric (Curcuma longa)", J. Ethanopha macol, 47, pp.59~63 (1995)
  63. Sen, P., "Machanisum of antistress activity of Ocimum sanctum Linn, eugenol and Tinospora malabarica in experimental animals", Indian J. Exp. Biol, 30, pp.596~605 (1992)
  64. Sen, P., "An experimental evaluation of Azadirachta indica in normal and stressed rats and adaptogenic effect", Abstract, World Neem Conference (1993)
  65. Shao, J-Z., "Aeromonas hydrophila induced apoptosis in Carassius auratus lymphocytes in vitro", Aquaculture, 229, pp.11~23 (2004)
  66. Shotts, E.B., "Medium for the isolation of Aeromonas hydrophila", App. Microbiol, 26, pp.550~553 (1973)
  67. Singh, K.P., "Experimental and clinical studies on Tinospora coradifolia", J. Int. Med. Res, 10, pp.9~12 (1999)
  68. Singh, S., "Evaluation of antiinflammatory activity of fatty acids of O. sanctum fixed oil", Indian J. Exp. Biol, 35, pp.380~383 (1997)
  69. Soltani, M., "Occurrence of a motile Aeromonas septicaemia in the imported ornamental fish, oscar Astronotus ocellatus: Isolation characterization and pathogenicity", J. Fac. Med. Uni. Tehran, 53, pp.63~65 (1998)
  70. Teh, S.J., "Histopathological biomarkers in feral freshwater fish populations exposed to different types of contaminant stress", Aquat. Toxicol, 37, pp.51~70 (1997)
  71. Thophon, S., "Histopathological alterations of white seabass, Lates calcarifer in acute and subchronic cadmium exposure", Environ. Poll, 121, pp.307~320 (2003)
  72. Thuy, N.T.T., "Studies on the immunostimulatory effect of certain plant extracts on fish", The Sixth Indian Fisheries Forum, CIFE, Mumbai, India, Abstracts AH-13, 통권 : 153 (2002)
  73. Van der Nat, J.M., "Immuomodulatory activity of an aqueous extract of Azadirachta indica stem bark", J. Ethanopharmacol, ㅍ, pp.125~131 (1987)
  74. Ventura, M.T., "Lesions associated with natural and experimental infections of Aeromonas hydrophila in channel catfish, Ictalurus puntatus Rafinesque", J. Fish Dis, 11, pp.397~407 (1988)
  75. Whittington, R.J., "Epizootic mortality in the pilchard Sardinops sagax neopilchardus in Australia and New Zealand in 1995", I. Pathology and epizootiology. Dis. Aquat. Org, 28, pp.1~16 (1997)
  76. Yadav, M., "Cytotoxin elaboration by Aeromonas hydrophila isolated from fish with epizootic ulcerative syndrome", J. Fish Dis, 15, pp.183~189 (1992)

관련 논문AI추천

Assessment of the Risk of Microplastics on Gill and Gut Health and Subsequent Pathogen Susceptibility in the Goldfish ModelHwang Sungmin, Hyun Jimin, Kim Ho Sung, Moon Ki Hwan, Park Jeong Woo, Ryu BoMi, Yerke Aaron M., Yoon Yongjoon, Yun Bohyun한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권 Effects of Prunella vulgaris labiatae extract on specific and non-specific immune responses in tilapia (Oreochromis niloticus)박관하, 최상훈한국축산학회 · 한국축산학회지2014 · 제56권 제1호Efficacy of dietary propolis and its nanoparticles on immune- response, stress indicators, and prevention of Pseudomonas aeruginosa infection in Oreochromis niloticusAhmed Farghali, Asmaa N. Mohammed, Fatma M. M. Korni, Nour El-Houda y. Hassan, Rehab K. Mahmoud, Usama K. Moawad수산생명의학회 · 한국어병학회지2023 · 제36권 제1호The dietary effect of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus강인성, 강태윤, 박관하, 방석진, 전세영, 정상목, 최상훈, 최재혁, 황요셉수산생명의학회 · 한국어병학회지2019 · 제32권 제1호Antimicrobial and antioxidant activity of some Indian medicinal plants for the protection against fish pathogenic bacteria김만철, 김주상, 장익수, 허문수, Chellam Balasundaram, Ramasamy Harikrishnan, Sundaram Jawahar수산생명의학회 · 한국어병학회지2009 · 제22권 제3호천심련(Andrographis paniculata) 추출물이 넙치(Paralichthys olivaceus)의 장에 미치는 조직학적 영향 평가김성현, 박영진, 이연경, 이주노, 임새연, 한소리, 홍지민수산생명의학회 · 한국어병학회지2024 · 제37권 제2호

이 간행물의 다른 논문 (5건)