Antimicrobial and antioxidant activity of some Indian medicinal plants for the protection against fish pathogenic bacteria
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This study has shown the screening of anti-bacterial activity of three Indian medicinal plant choloroform : methanol (50:50) solvent leaf extracts (i.e. Azadirachta indica, Ocimum sanctum, and Curcuma longa) with different concentrations (10, 5, 2.5, 1.25, 0.625, 0.312, and 0.156 mg/ml) under in vitro conditions against fish pathogenic bacteria, Aeromonas hydrophila, Streptococcus iniae, Vibrio harveyi, V. anguillarum, and Edwardsiella tarda isolated from olive flounder farms, Jeju Island, South Korea. The anti-microbial activity of the A. indica and O. sanctum extracts yielded the zones of growth inhibition (ZI) was 3 and 1mm against A. hydrophila at concentration of 0.156 mg/ml when compared to that of tetracycline standard (3 mm). At highest concentration (10 mg/ml) of A. indica, O. sanctum, and C. longa, high inhibition was 9, 7, and 6mm when compared to that of tetracycline (11 mm) against A. hydrophila. The minimum inhibitory concentration (MIC) of A. indica, O. sanctum, and C. longa at 0.156 mg/ml that yield 9, 10, and 13 CFU/ml for A. hydrophila, 16, 22, and 25 CFU/ml for S. iniae and 18, 22, and 23 CFU/ml for E. tarda compared to the tetracycline. At highest concentration (10 mg/ml) of the three extracts was better inhibiting the growth of A. hydrophila, S. iniae and E. tarda. A. indica, O. sanctum, and C. longa were determined to the potential antioxidant activityon the basis of their scavenging activity of the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. A. indica extract was 0.625 mg/ml which indicated that the strong anti-oxidant activity. However, O. sanctum and C. longa extracts showed weak anti-oxidant activity at this concentration. Hence, in vitro assay among the pathogens, A. hydropila is better inhibitory activity of the extracts. It is evident that the Indian medicinal plants extracts were subjected to its effectiveness against A. hydrophila, S. iniae, and E.tarda at low concentrations. The obtained results in the present study suggested that the Indian plant extracts is a prevention tools for Korean olive flounder aquaculture pathogens and its need further advance investigation.
Abstract
This study has shown the screening of anti-bacterial activity of three Indian medicinal plant choloroform : methanol (50:50) solvent leaf extracts (i.e. Azadirachta indica, Ocimum sanctum, and Curcuma longa) with different concentrations (10, 5, 2.5, 1.25, 0.625, 0.312, and 0.156 mg/ml) under in vitro conditions against fish pathogenic bacteria, Aeromonas hydrophila, Streptococcus iniae, Vibrio harveyi, V. anguillarum, and Edwardsiella tarda isolated from olive flounder farms, Jeju Island, South Korea. The anti-microbial activity of the A. indica and O. sanctum extracts yielded the zones of growth inhibition (ZI) was 3 and 1mm against A. hydrophila at concentration of 0.156 mg/ml when compared to that of tetracycline standard (3 mm). At highest concentration (10 mg/ml) of A. indica, O. sanctum, and C. longa, high inhibition was 9, 7, and 6mm when compared to that of tetracycline (11 mm) against A. hydrophila. The minimum inhibitory concentration (MIC) of A. indica, O. sanctum, and C. longa at 0.156 mg/ml that yield 9, 10, and 13 CFU/ml for A. hydrophila, 16, 22, and 25 CFU/ml for S. iniae and 18, 22, and 23 CFU/ml for E. tarda compared to the tetracycline. At highest concentration (10 mg/ml) of the three extracts was better inhibiting the growth of A. hydrophila, S. iniae and E. tarda. A. indica, O. sanctum, and C. longa were determined to the potential antioxidant activityon the basis of their scavenging activity of the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. A. indica extract was 0.625 mg/ml which indicated that the strong anti-oxidant activity. However, O. sanctum and C. longa extracts showed weak anti-oxidant activity at this concentration. Hence, in vitro assay among the pathogens, A. hydropila is better inhibitory activity of the extracts. It is evident that the Indian medicinal plants extracts were subjected to its effectiveness against A. hydrophila, S. iniae, and E.tarda at low concentrations. The obtained results in the present study suggested that the Indian plant extracts is a prevention tools for Korean olive flounder aquaculture pathogens and its need further advance investigation.
참고문헌 (49)
- Adams, A., "The potency of adjuvanted vaccines in rainbow trout, Salmo gairdneri Richardson, with whole cell bacterins by oral and intraperitoneal routes", J. Fish Dis., 6, pp.129~134 (1998)
- Alves, T.M.A., "Biological screening of Brazilian medicinal plants", The J Memorias do Instituto Oswaldo Cruz, 95, pp.367~373 (2000)
- Anderson, D.P., "Immunostimulants, adjuvants, and vaccine carriers in fish: applications in aquaculture", Ann. Rev. Fish Dis., 2, pp.281~301 (1992)
- Anto, R.J., "Antimutagenic and anticarcinogenic activity of natural and synthetic curcuminoids", Mutation Res., 370, pp.127~131 (1996)
- Babu, B., "Antibacterial activity of different plant extracts", Indian J. Microbiol., 42, pp.361~363 (2000)
- Se Chang Park, "Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island", Journal of Veterinary Science, 7(1), pp.53~58 (2006), 대한수의학회
- Bauer, A.W., "Antibiotic susceptibility testing by a standardized single disk method", Am. J. Clim. Pathol., 45, pp.493~497 (1966)
- Bowden, T.J., "Long-term study of antibody response and injection-site effects of oil adjuvants in Atlantic halibut (Hippoglossus hippoglossus L.)", Fish Shellfish Immunol., 14, pp.363~369 (2003)
- Collins, C.H., "Microbiological methods, 6th edition" (1989), Butterworth
- Cox, J.C., "Adjuvantsda classification and review of their modes of action", Vaccine, 15, pp.248~256 (1997)
- Cuendet, M., "Iridoid glucosides with free radical-scavenging properties from Fagraea blumei", Helvetica Chimica Acta., 80, pp.1144~1152 (1997)
- Devakumar, C., "Chemistry, In: Neem", pp.77~110 (1996), Society of Pesticide Science
- Evans, W.C., "Trease and Evans Pharmacognosy, 14th edition" (1996), W.B. Saunders Co. Ltd.
- Gupta, S.K., "Validation of traditional claim of Tulsi Ocimum sanctum Linn. as a medicinal plant", Indian J. Exp. Biol., 40, pp.765~773 (2002)
- Harikrishnan, R., "Antimicrobial activity of medicinal herbs in vitro against fish pathogen, Aeromonas hydrophila", Fish Pathol., 40, pp.187~189 (2005)
- Harikrishnan, R., "Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophila infection", Aquaculture, 221, pp.41~50 (2003)
- Hunter, P.A., "The current status of surveillance of resistance to antimicrobial agents: report on a meeting", J. Antimicrob. Chemother, 49, pp.17~23 (2002)
- Iwalokun, B.A., "Shigellocidal properties of three Nigerian medicinal plants: Ocimum gratissimum, Terminalia avicennoides, and Momordica balsamina", J. Health Popul. Nutr., 19, pp.331~335 (2001)
- Janssen, A.M., "Antimicrobial acitivities of some Ocimum species grown in Rwanda", J. Ethnopharmacol., 26, pp.57~63 (1989)
- Joseph, S.W., "The isolation, identification, and systematics of the motile Aeromonas species", Ann. Rev. Fish Dis., 4, pp.315~343 (1994)
- Kikuzaki H., "Antioxidant Effects of Ginger Constituents", J. Food Sci., 58, pp.1407~1410 (1993)
- Kim, D.H., "Bacterial enteritis and the development of the larval digestive tract in olive flounder, Paralichthys olivaceus (Temminck & Schlegel)", J. Fish Dis., 27, pp.497~505 (2004)
- Larson, R.A., "The antioxidants of higher plants", Phytochemistry, 27, pp.969~978 (1988)
- Lawrence, G.W., "Phase I trial in humans of an oil-based adjuvant SEPPIC MONTANIDE ISA 720", Vaccine, 15, pp.176~178 (1997)
- 이훈구, "2002-2003년도 복수증 양식산 넙치로부터 동정된 미생물상", 미생물학회지, 40(2), pp.133~138 (2004), 한국미생물학회
- Logambal, S.M., "Immunostimulatory effect of Azadirachtin in Oreochromis mossambicus (Peters)", Indian J. Exp. Biol., 38, pp.1092~1096 (2000)
- Mandal, S., "R factor in Proteus vulgaris from ulcerative disease of fish, Channa punctatus", Indian J. Exp. Biol., 40, pp.614~616 (2002)
- Mitra, C.R., "Identification of nimbidic acid and nimbidinin from Azadirachta indica", Photochemistry, 10, pp.857~860 (1971)
- Mukherjee, D., "lmpairment of steroidgenesis and reproduction in sexually mature Cyprinus carpio by phenol and sulfide under laboratory conditions", Aquat. Toxicol., 21, pp.29~40 (1991)
- Nakamura, C.V., "Antibacterial activity of Ocimum gratissimum L essential oil", J Memorias do Instituto Oswaldo Cruz, 94, pp.675~678 (1999)
- National Committee for Clinical Laboratory Standards, "Methods for dilution antimicrobial susceptibilitytests for bacteria that grow aerobically. 3rd ed", Natl. Committee Clin. Lab. Stand., ~ (1997)
- National Committee for Clinical Laboratory Standards, "Performance standards for antimicrobial susceptibility testing: tenth informational supplement M100-S10", NCCLS, ~ (2000)
- Njiro, S. M., "Effect of an aqueous extract of Azadirachta indica on the immune response in mice", Onderstepoort. J. Vet. Res., 66, pp.59~62 (1999)
- Oh, M.J., "Change of pathogenicity in olive flounder Paralichthys olivaceus by co-infection of Vibrio harveyi, Edwardsiella tarda and marine birnavirus", Aquaculture, 257, pp.156~160 (2005)
- Pike, J., "Effect of vitamin and trace element supplementation on immune indices in healthy elderly", Int. J. Vitam. Nutr. Res., 65, pp.117~120 (1995)
- Rasadah, M.A., "Antimicrobial activity of some species of Bignoniaceae", ASEAN Review of Biodiversity and Environmental Conservation (ARBEC), ~ (2007), www.arbec.com.my[DOI]
- Reddy, R.C., "Curcumin for malaria therapy", Biochem. Biophys. Res. Commun., 326, pp.472~474 (2005)
- Rojanapo, W., "Carcinogenicity of an oxidation product of pphenylenediamine", J. Sci. Thailand, 11, pp.177~188 (1985)
- Sadhu, S.K., "Seperartion of leucas, aspara amedicinal plant of Bangladesh, guided by prostaglandin inhibitory and anti oxidant activities", Chem. Pharm. Bull., 51, pp.595~598 (2003)
- Satoskar, R.R., "Evaluation if anti-inflammatory properties of curcumin (diferuloylmethane) in patients with postoperative inflammation", Int. J. Clim. Pharmacol. Ther. Toxicol., 24, pp.651~654 (1986)
- Skaltsa, H., "Flavonoids from Lippia citriodora", Planta Med., 54, pp.465~ (1988)
- Soliman, K.M., "Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi", Food Chem. Toxicol., 40, pp.1669~1675 (2002)
- Uddin, S.N., "Antioxidant and Antibacterial Activities of Senna tora Roxb", Am. J. Plant Physiol., 3, pp.96~100 (2008)
- Vaidyanathan, P.S.V., "Indian Medicinal plants Arya Vaidya Sala, Kottaail" (1995), Orient Longman Ltd.
- Valentao, P., "Studies on the antioxidant activity of Lippia citriodora infusion: scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid", Biol. Pharm. Bull., 25, pp.1324~1327 (2002)
- Venkatalakshmi, S., "Immunostimulation by leaf extract of Ocimum sanctum Linn. in Oreochromis mossambicus (Peters)", J. Aquacultu. Tropics, 16, pp.1~10 (2001)
- Wan, J., "The effect of essential oils of basil on the growth of Aeromonas hydrophila and Pseudomonas fluorescens", J. Appl. Microbiol., 84, pp.152~158 (1998)
- World Health Organization, "Traditional Medicine Strategy 2002-2005", World Health Organization, ~ (2002)
- Zhang, X., "Traditional medicine: its importance and protection In: Protecting and Promoting Traditional Knowledge: Systems, National Experiences and International Dimensions. Part 1. The Role of Traditional Knowledge in Healthcare and Agriculture", pp.3~6 (2004), United Nations