천심련(Andrographis paniculata) 추출물이 넙치(Paralichthys olivaceus)의 장에 미치는 조직학적 영향 평가
Evaluation of the histological effects of Andrographis paniculata extract on the intestine of olive flounder (Paralichthys olivaceus)
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초록
Andrographis paniculata is a medicinal plant with pharmacologically active components. It is widely used in various treatments due to their ability for immune stimulation, anti-inflammation, antibacterial, antioxidant, and liver protection. In this study, olive flounder (length 15±1.5 cm, weight 20±2.3 g) were fed with either commercial flounder feed (Control) or control feed supplemented with 0.2% A. paniculata extract (GBL) for 36 days. Its effects were evaluated under oral infection with E. leei (10 2 cells/fish) through survival rate and histology analysis of intestinal tissues. At the end of experi- ment, survival rate of GBL groups had lower mortality compared to those of control group. For the histological analysis, under infection with E. leei, the count of goblet cells significantly increased in GBL groups compared to the control group. Villus length, width and muscularis thickness were significantly increased in GBL group. In addition, the GBL group had a significant reduction in lamina propria width under the infection condition. These results suggest that the use of A. paniculata extract as a feed additive can improve intestinal health of olive flounder.
Abstract
Andrographis paniculata is a medicinal plant with pharmacologically active components. It is widely used in various treatments due to their ability for immune stimulation, anti-inflammation, antibacterial, antioxidant, and liver protection. In this study, olive flounder (length 15±1.5 cm, weight 20±2.3 g) were fed with either commercial flounder feed (Control) or control feed supplemented with 0.2% A. paniculata extract (GBL) for 36 days. Its effects were evaluated under oral infection with E. leei (10 2 cells/fish) through survival rate and histology analysis of intestinal tissues. At the end of experi- ment, survival rate of GBL groups had lower mortality compared to those of control group. For the histological analysis, under infection with E. leei, the count of goblet cells significantly increased in GBL groups compared to the control group. Villus length, width and muscularis thickness were significantly increased in GBL group. In addition, the GBL group had a significant reduction in lamina propria width under the infection condition. These results suggest that the use of A. paniculata extract as a feed additive can improve intestinal health of olive flounder.
참고문헌 (32)
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