해산어류에서 분리된 Vibrio harveyi의 혈청학적 특성과 항원성
Serological characteristics and antigenicities of Vibrio harveyi isolated from marine cultured fish
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초록
V. harveyi는 양식산업에서 중요한 해산 양식 어류와 새우류에 많은 폐사를 일으키는 병원체 로 알려져 있다. 본 연구에서는 해산 양식어류 에서 분리된 11개 strain의 Vibrio harveyi의 생화 학적, 생리학적, 혈청학적, 면역학적 특성을 조사 하였다. 생화학적 특성과 TCBS agar에서 집락의 색깔이 달라 표현형의 차이가 있었고 V. harveyi 분리균주들은 편모항원에 의해 4개 이상의 혈청 형으로 구분되었다. 어류에서 분리된 V. harveyi 는 western blotting으로 26~34 kDa 사이에 동일 한 크기의 major protein을 갖는 group A로 구분 되었고 넙치에 대하여 표준균주인 V. harveyi KCCM40866 보다 강한 병원성을 갖고 있었다. V. harveyi C05011로 면역 유도된 넙치는 다른 group A 균주를 인위감염 시켰을 때 높은 항병력을 나타내었다.
Abstract
Vibrio harveyi was a significant pathogenic agent and cause a high mortality of cultured fish and shrimp in the aquaculture industry. In this study, we have investigated biochemical, physiological, serological and immunological characteristics of V. harveyi isolated from marine cultured fish. The enotypes of V. harveyi were differentiated with their own biochemical characteristics and colors of colony on the TCBS agar. V. harveyi were classified into more than four serogroups by agglutination test. Most isolates were classified into a group A which is categorized with the same band pattern generated by western blotting. Group A was characterized by a major protein, which is ranged from 26 and 34 kDa in size, and has a virulence to oliver flounder more than reference strain KCCM40866. Oliver flounders vaccined with FKC of V. harveyi C05011 were highly resistant to infection by other strains of group A.
참고문헌 (23)
- Abbott, J.D., "Serotyping of Campylobacter jejuni", coli, 1980, pp.33, 762~766
- Bachrach, G., "Recovery of Streptococcus iniae from diseased fish Previously vaccinated with a Streptococcus vaccine", Applied and Environmental Microbiology, 67, pp.3756~3758 (2001)
- Balebone, M.C., "Effectiveness of a divalent vaccine for sole, Solea senegalensis (Kaup), against Vibrio harveyi and Photobacterium damselae subsp. piscicida", J. Fish Dis., 28, pp.33~38 (2005)
- Chiou, C.S., "Vibrio parahaemolyticus serovar O3:K6 as cause of unusually high incidence of food-borne disease outbreaks in Taiwan from 1996 to 1999", J. Clinical Microbiology, 38, pp.4621~4625 (2000)
- Fouz, B., "Isolation of a new serovar of Vibrio vulnificus pathogenic for eels cultured in freshwater farms", Aquaculture, 217, pp.677~682 (2003)
- Gil, A., "O3:K3 Serotype of Vibrio parahaemolyticus identical to the global panademic clone associated with diarrha in Peru", Int. J. Infect. Dis., 11, pp.324~328 (2007)
- Karunasagar, I., "Mass mortality of Penaeus monodon larvae due to antibiotic resistant Vibrio harveyi infection", Aquaculture, 128, pp.203~209 (1994)
- Kerry, J., "Fish feed as a source of oxytetracycline resistant bacteria in the sediments under fish farm", Aquaculture, 131, pp.101~113 (1995)
- Kirov, S.M., "Aeromonas flagella (polar and lateral) are enterocyte adhesins that contribute to biofilm formation on surfaces", Infect. Im-mun., 72, pp.1939~1945 (2004)
- Lavilla-Pitogo, C. R., "Occurrence of luminous bacterial disease of Penaeus monodon larvae in the Philippines", Aquaculture, 91, pp.1~13 (1990)
- Obata-Yasuoka, M., "Vaginal Escherichia coli share common virulence factor profiles, serotypes and phylogeny with other extrain testinal E. coli", Microbiol, 148, pp.2745~2752 (2002)
- Ortigosa, M., "Numerical taxonomy of Vibrionaceae isolated from oysters and seawater along an annual cycle", System. Appl. Microbiol., 17, pp.216~225 (1994)
- Park, S.I., "Serotype and virulence of Edwardsiella tarda isolated from eel and their environment", Fish Pathol., 18, pp.85~89 (1983)
- Pizzutto, M., "Classification of isolates of Vibrio harveyi virulent to Penaeus monodon larvae by protein profile analysis and M13 DNA fingerprinting", Dis. Aquat. Org., 21, pp.61~68 (1995)
- Santos, Y., "Analysis of antigens present in the extracellular products and cell surface of Vibrio anguillarum serotypes O1, O2, and O3", Appl. Environ. Microbiol., 61, pp.2493~2498 (1995)
- Shinoda, S., "Lateral flagellar antigen of Vibrio algionlyticus and Vibrio harveyi: Existence of serovars common to the two species", Microbiol. Immunol., 29, pp.173~182 (1985)
- Shinoda, S., "Lateral flagella of vibrios: serological classification and genetical similarity", Microbiol. Immunol., 36, pp.303~309 (1992)
- Soffientino, B., "Go′mez-Chiarri, M", Dis. Aquat. Org., 38, pp.201~210 (1999)
- Sunaryanto, A., "Occurrence of pathogenic bacteria causing luminosense in penaeid larvae in Indonesian hatcheries", Bull. Brackishwater Aquaculture, 8, pp.105~112 (1986)
- Wimpee, C.F., "Development of Species-Specific Hybridization Probes for Marine Luminous Bacteria by Using In Vitro DNA Amplification", Appl. Environ. Microbiol., 57, pp.1319~1324 (1991)
- 정승희, "2000년~2006년 하절기 양식어류의 병원체 감염현황", 한국어병학회지, 19(3), pp.207~214 (2006), 한국어병학회
- 박수일, "배양 조건에 따른 Vibrio harveyi extracellular products (ECPs)의 특성", 한국어병학회지, 19(2), pp.119~126 (2006), 한국어병학회
- 최정현, "조피볼락에 대한 Vibrio harveyi의 병원성", 한국어병학회지, 20(2), pp.99~108 (2007), 한국어병학회