본문 바로가기
KERD
논문KCI 등재

질병의 증상을 보이는 해수 양식 어류에서 분리한 비브리오속 세균

Vibrios isolated from diseased marine culturing fishes in Korea

원경미|조미영|이화|김명석|최광진|박수일|우승호|김은전|김수미
수산생명의학회 (Aquatic Life Medical Science)|2005.08|한국어병학회지 (JOURNAL OF FISH PATHOLOGY)|제18권 제2호|pp. 133-145

2

조회

0

다운로드

12

인용

0

참고문헌

초록

본 연구에서는 2002년에서 2004년간 우리 나라 해수 어류 양식장에서 분리되는 세균 중 비브리오 속에 속하는 세균의 종 조성을 조사하였다. 질병의 증상을 보이는 어류로부터 166개의 비브리오속 세균 균주를 수집하였으며, 이들 균주는 넙치 (133 균주), 조피볼락 (8 균주), 참돔 (6 균주), 대하 (5 균주), 감성돔 (4 균주), 전복 (3 균주) 및 기타 해수 어류 (7 균주) 등에서 분리한 균주를 대상으로 하였다. 모든 분리 균주에 대하여 각종 생화학적 성상을 조사하고 그 특성에 따라 분리 균주를 구분하였다. 각 생화화적 성상 group에서 대표 균주를 선정하여 16S rRNA 및 16S-23S rRNA 유전자 서열을 분석하고 이들 결과를 종합하여 분리 균주을 동정하였다. 그 결과 14종 이상의 비브리오 종으로 동정할 수 있었으며, 그 종 조성은 V. ichthyoenteri (45 strains), V. alginolyticus (34 strains), V. harveyi (32 strains), Ph. damselae subsp. damselae (Formerly V. damsela, 10 strains), V. campbellii (6 strains), V. costicola-like (6 strains), V. fisheri (5 stains), V. fluvialis (4 strains) 및 Vibrio spp. (24 strains) 등이었다.

Abstract

A bacteriological survey in maricultured fish farms was conducted in Korea from 2002 to 2004. A total number of 166 Vibrio isolates were collected from diseased fishes, including olive flounder (133 isolates), black rock fish (8 isolates), red sea bream (6 isolates), shrimp (5 isolates), black sea bream (4 isolates), abalone (3 isolates) and other fishes (7 isolates). All isolates were phenotypically characterized and then groups were obtained using the traditional biochemical test. Representative isolates of each group were genotypically characterized with sequencing the 16S rRNA genes or 16S-23S intergenic space genes. Above 14 species of Vibrio were identified as V. ichthyoenteri (45 strains), V. alginolyticus (34 strains), V. harveyi (32 strains), Ph. damselae subsp. damselae (Formerly V. damsela, 10 strains), V. campbellii (6 strains), V. costicola-like (6 strains), V. fisheri (5 stains), V. fluvialis (4 strains) and others Vibrio sp. (24 strains) by combining of biochemial and genetic characteristics.

참고문헌 (47)

  1. "Improvement and update of a set of keys for biochemical identification of Vibrio species", pp.719~721 (1994)
  2. "Phylogenetic identification and in situ detection of individual microbial cells without cultivation", 59, pp.143~169 (1995)
  3. "Vibrio pelagius associated with mortalities in farmed turbot", pp.129~133 (1992)
  4. "Diseases of farmed and wild fish" (1999), Springer-Praxis Publishing Ltd
  5. "A probiotic strain of Vibrio alginolyticus effective in reducing disease caused by Aeromonas salmonicida", 18, pp.93~96 (1995)
  6. "Studies in chemical control of luminous bacteria Vibrio harveyi and Vibrio splendidus isolated from diseased Penaeus monodon larvae and rearing water", pp.133~139 (1990)
  7. "Survey of bacterial pathologies affecting farmed gilt-head sea bream in southwestern Spain from 1990 to 1996", 166, pp.19~35 (1998)
  8. "Numerical classification of species of Vibrio and related genera", pp.437~467 (1986)
  9. "Development of monoclonal antibodies that identify vibrio species commonly isolated from infections of humans" (1992)
  10. "Microbiology of summer flounder Paralichthys dentatus fingerling production at a marine fish hatchery", 211, pp.9~28 (2002)
  11. "List of bacterial names with standing in nomenclature a folder available on the Internet", 47, pp.590~592 (1997)
  12. "Dominant intestinal microflora of sea bream and sea bass larvae", 155, pp.387~399 (1997)
  13. "New approaches to typing and identification of bacteria using the 16S-23S rDAN spacer region", 142, pp.3~6 (1996)
  14. "Studies on the chemical control of a Vibrio campbellii-like bacterium affecting hatchery-reared Penaeus indicus larvae", pp.1~9 (1994)
  15. "Bergey's manual of determinative bacteriology 9th ed", pp.260~289 (1994), Williams and Wilkins Co
  16. "Vibrios in the marine and estuarine environments", 3, pp.60~63 (1995)
  17. "a pathogen of Japanese flounder", 46, pp.155~159 (1996)
  18. "A study on the characteristics of bacteria isolated from cultured flounder showing disease symptoms in Jeju area of Korea" (2003), Cheju National University
  19. "Mannual of clinical microbiology 5th ed", pp.384~395 (1991)
  20. "Organization of the bacterial chromosome", pp.502~539 (1990)
  21. "Bacterial enteritis and the development of the larval digestive tract in olive flounder", 27, pp.497~505 (2004), Temminck & Schlegel
  22. "Visceral neoplasia and epidermal papillomas in cultured turbot Scophthalmus maximus", pp.179~187 (1990)
  23. "Occurrence of luminous bacterial disease of Penaeus monodon larvae in the Philippines", pp.1~13 (1990)
  24. "Virulence of Vibrio alginolyticus isolated from diseased tiger prawn", 32, pp.229~231 (1996)
  25. "Isolation of Vibrio species from cultured flounder with ulcers and Ascites in the Southern coast of Korea during the winter season", pp.319~328 (1991)
  26. "Microbial flora in ascitic cultivated olive flounder in Koe-je Island in Korea during 2002-2003", 40, pp.133~138 (2004)
  27. "Disease of cultured penaeid shrimp CRC Handbook of Mariculture", pp.393~486 (1993), CRC Press
  28. "Survival of Vibrio splendius biotype I in seawater Bulletin of the European Association of Fish Pathologists", 15, pp.70~72 (1995)
  29. "causes skin lesions on the damselfish Chromis punctipinnis", pp.1139~1140 (1981)
  30. "Individual biochemical tests.Biochemical tests for identification of medical bacteria", pp.1~456 (2000), Lippincott Williams and Wikins
  31. "Detection of Vibrio anguillarum from water", pp.641~647 (1986)
  32. "Vibrio ordalii isolated from diseased ayu", pp.239~242 (1986)
  33. "Isolation of Vibrio anguillarum from juvenile red sea-bream", pp.211~214 (1982)
  34. "Vibrio anguillarum infection in juveniles of tiger puffer", pp.29~30 (1987)
  35. "Bacterial problems associated with Scallop Pacten maximus larval culture", 27, pp.67~76 (1996)
  36. "Taxonomic evidence that Vibrio carchariae Grimes et al 1985 is junior synonym of Vibrio harveyi", 48, pp.749~758 (1998)
  37. "Molecular characterization of Vibrio anguillarum biotype 2", pp.1011~1018 (1981)
  38. "a causative agent of vibriosis in fish", (v), pp.343~348 (1981)
  39. "Microbial origin of the abdominal swelling affecting farmed larvae of gilt-head seabream", 27, pp.323~333 (1996)
  40. "Bacterial flora of East China sea and Yosu costal sea areas Horizontal distributions of bacteria isolated from the sea area", 29, pp.17~25 (1996)
  41. "Evaluation of the genus Listonella and reassignment of Listonella damsela MacDonell and Colewell to the genus photobacterium as Photobacterium damsela comb with an emended description", (v), pp.529~534 (1991)
  42. "Infectious necrotizing enteritis and mortality caused by Vibrio carchariae in summer flounder Paralichthys dentatus during intensive culture", 38, pp.201~210 (1999)
  43. "Occurrence of a pathogenic bacteria causing luminescence in penaeid larvae in Indonesian hatcheries", pp.105~112 (1986)
  44. "Improving the sensitivity of progressive multiple sequence alignment through sequence weighing", pp.4673~4680 (1994)
  45. "Vibrio anguillarum infection in flounder", yama yasu(i bu), pp.69~70 (1988)
  46. "Isolation and characterization of Vibrio carchariae a causative agent of gastroenteritis in the groupers", 35, pp.109~115 (1997)
  47. "Bacterial recovered from diseased cultured gilthead sea bream", 218, pp.11~20 (2003)

관련 논문AI추천

미성어 양식 넙치, Paralichthys olivaceus에서 분리한 Vibrio icthyoenteri의 표현형 및 유전자형 특성이화, 김수미, 박수일수산생명의학회 · 한국어병학회지2006 · 제19권 제2호넙치, Paralichthys olivaceus에서 병원성 Photobacterium damselae subsp. damselae의 분리권문경, 박상언, 박수일, 방종득수산생명의학회 · 한국어병학회지2005 · 제18권 제3호해산어류에서 분리된 Vibrio harveyi의 혈청학적 특성과 항원성김명석, 김진우, 박명애, 오윤경, 정현도, 조지영수산생명의학회 · 한국어병학회지2009 · 제22권 제1호Identification of Vibrio species isolated from cultured olive flounder (Paralichthys olivaceus) in Jeju Island, South Korea김정은, 손한창, 이제희, Changnam Jin한국수산과학회 · Fisheries and Aquatic Sciences2019 · 제22권 제3호2005년부터 2006년 사이 우리나라 양식 넙치, Paralichthys olivaceus의 세균성 질병에 대한 역학 조사권문경, 김명석, 김진도, 박경현, 박명애, 박신후, 오윤경, 이덕찬, 이주석, 이창훈, 조미영, 지보영, 최동림, 최혜승수산생명의학회 · 한국어병학회지2007 · 제20권 제1호양식 전복 (Haliotis discus hannai)으로부터 분리된 Vibrio harveyi의 생화학적 특 성 및 병원성김명석, 김진도, 도정완, 원경미, 이남실, 이덕찬, 정승희, 조미영, 진세윤한국환경생물학회 · 환경생물2017 · 제35권 제4호

이 간행물의 다른 논문 (8건)

어류 빈혈에 관한 병리학적 고찰이무근, 허민도, 송나영pp. 105-118Co-infection of two myxosporean parasites - Parvicapsula anisocaudata and an unidentified myxosporean - in the kidney of cultured olive flounder, Paralichthys olivaceus허민도, 김기홍, Mu-Kun Lee, 조재범pp. 119-124Neoparamoeba sp. infection on gills of olive flounder, Paralichthys olivaceus in Korea조재범, 김형준, 김기홍, 허민도, Mu-Kun Leepp. 125-131한국산 해조류의 어류병원성세균에 대한 항균활성신종암, 오명주, 강소영pp. 147-156Nocardia 감염증에 의한 양식 가물치의 대량 폐사박명애, 이덕찬, 조미영, 최희정, 김진우pp. 157-165
원문 보기