배양 조건에 따른 Vibrio harveyi extracellular products (ECPs)의 특성
Characteristics of the extracellular products (ECPs) of Vibrio harveyi grown under various conditions
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초록
본 연구에서는 Vibrio harveyi의 주요 병원성 인자로 알려져 있는 ECPs를 분리하는 적정 조건을 제시하고자 하였다. 5 개의 분리균주 (FF8, FF10, FR1, FR2 and FT1 strain)와 2 개의 참조균주 (ATCC 35084 및 ATCC 14126)의 ECPs를 다양한 조건에서 분리해 본 결과, 1.5% NaCl 첨가 TSA 배지에서 25℃, 24 시간 배양하는 것이 가장 좋으며, ECPs 분리시의 buffer system은 pH 8.0이 적정한 것으로 나타났다. 나아가 우리 나라에서 분리한 5 개의 분리 균주들의 주요 ECPs 성분이 serine protease인 것으로 조사되었다.
Abstract
The characteristics of the extracellular products (ECPs) of Vibrio harveyi grown under various conditions were studied. The extracellular products (ECPs) of Vibrio harveyi is well known as an important pathogenic factor. The optimal isolation condition of the V. harveyi ECPs was incubation with 1.5% NaCl added TSA medium at 25℃ for 24 h. The buffer system for ECPs isolation controlled as pH 8.0 displayed optimal condition. The major protein of ECPs isolated from the five V. harveyi strains originated from Korea (FF8, FF10, FR1, FR2 and FT1 strain) were revealed to serine protease.
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