본문 바로가기
KERD
논문KCI 등재

Vibrio harveyi의 미생물학적 특성

Microbiological characteristics of Vibrio harveyi

2

조회

0

다운로드

3

인용

0

참고문헌

초록

본 연구에서는 우리 나라에서 분리된 Vibrio harveyi의 미생물학적 특성을 조사하여 V. harveyi 연구의 기초적인 정보를 제공하고자 하였다. V. harveyi는 염분 농도 3%, 배양 온도 30℃에서 가장 잘 증식하였다. Swarming 운동은 1.5% NaCl 첨가 TSA에서 가장 활발하였으며, swarming 운동시 균체는 길어지며, 짧고 곧은 다수의 lateral flagella가 생성된 것을 확인하였다. 우리나라에서 분리된 V. harveyi는 luminometer로 직접 검출하는 방법과 luciferase gene을 검출하는 방법 모두에서 음성으로 나타나, 발광능이 없는 것으로 조사되었다. V. harveyi의 균체 단백질은 50 kDa 부근의 major protein을 가지며, 균주에 따라 40 kDa 근처의 상이한 band pattern을 보였다.

Abstract

In 1999, Vibrio harveyi infection occurred among cultured marine finfishes including olive flounder (Paralichthys olivaceus), black rockfish (Sebastes schlegeli), and turbot (Scophtalmus maximus) in the province of Gyeongsang, Korea. We examined the various microbiological characteristics of this V. harveyi strains. V. harveyi was grown well in the 3% NaCl at 30℃. The swarming activity appeared to be an one of the characteristic properties of the V. harveyi was the highest in the 2% NaCl concentration at TSA medium. These cells were elongated and had the several lateral flagella which is not waved and shorter than polar flagella. It was not luminescent in the all isolated strains. Whole cell of the V. harveyi had a major protein of 50 kDa and presented various band around of 40 kDa in strains.

참고문헌 (25)

  1. "Luminous and non-luminous Vibiro harveyi associated with shell disease in cultured Penaeus indicus", 10, pp.273~276 (1995)
  2. "Biology of the marine enterobacteria", pp.39~61 (1977)
  3. "Scanning electron microscope observation of the swarming phenomenon of Vibrio parahaemolyticus. J. Bacteriol.", pp.956~959 (1982)
  4. "An electron microscope study of Vibrio flagella. J. Gen. Microbiol.", pp.235~239 (1968)
  5. "Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators", 50, pp.727~751 (1996)
  6. "Mass mortality of Penaeus monodon larvae due to antibiotic-resistant Vibrio harveyi infection. Aquaculture", pp.203~209 (1994)
  7. "Bacteria that express lateral flagella enable dissection of the multifunctional roles of flagella in pathogenesis", 224, pp.151~159 (2003)
  8. "Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature", pp.680~685 (1970)
  9. "A comparison of three methods for assaying hydrophobicity of pathogenic Vibrios", 23, pp.343~346 (1996)
  10. "Atlas of Bacterial Flagellation", pp.1960~, New York
  11. "Environmental induced mortality of cultured grouper Epinephelus malabaricusinfected with high density of monogeneans and vibrios. In Programme and Abstracts of the second symposium on diseases in Asian aquaculture Health Section of the Asian Fisheries Society" (1993)
  12. "Infectious gastroenteritis caused by Vibrio harveyi (V. carchariae) in cultured red drum, Sciaenops ocellatus", 19, pp.59~61 (2003)
  13. "Isolation of Vibrio harveyi from diseased kuruma prawns Penaeus japonicus", 33, pp.129~132 (1996)
  14. "Dual flagellar systems enable motility under different circumstances", 7, pp.18~29 (2004)
  15. "Vibrio carchariae causes mass mortalities in Japanese abalone, Sulculus diversicolor supratexta", 33, pp.495~502 (1998)
  16. "Numerical taxonomy of Vibrionaceae isolated from oysters and seawater along an annual cycle. System. Appl. Microbiol.", pp.216~225 (1994)
  17. "Taxonomic evidence that Vibrio carchariae Grimes et al. 1985 is junior synonym of Vibrio harveyi (Johnson and Shunk 1936) Baumann et al. 1981", 48, pp.749~758 (1998)
  18. "Classification of isolates of Vibrio harveyi virulent to Penaeus monodon larvae by protein profile analysis and M13 DNA fingerprinting", 21, pp.61~68 (1995)
  19. "Association of Vibrio harveyi with mortalities in cultured marine fish in Kuwait", 136, pp.21~29 (1995)
  20. "Infectious necrotizing enteritis and mortality caused by Vibrio carchariae in summer flounder Paralichthys dentatus during intensive culture", 38, pp.201~210 (1999)
  21. "Characterization and ecological implication of luminous Vibrio harveyi isolated from tiger shrimp", penaeus mo(don), pp.217~220 (1993)
  22. "Giant flagellar bundles of Vibrio alginolyticus", pp.331~339
  23. "Development of species-specific hybridization probes for marine luminous bacteria by using in vitro DNA amplification. Appl. Environ. Microbiol. 57", pp.1319~1324 (1991)
  24. "Characterization of Vibrio harveyi, the causal agent of Vibriosis in cultured marine fishes in Korea", 9(3), pp.123~128 (2006)
  25. "Isolation and characterization of Vibrio carchariae, a causative agent of gastroenteritis in the groupers, Epinephelus coioides", 35, pp.109~115 (1997)

관련 논문AI추천

이 간행물의 다른 논문 (12건)