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톨트라주릴 합성유도체, N-(4-(4-Fluorophenoxy)-3-methylphenyl) acetamide 근육 주사에 따른 넙치의 자연살해세포 (Natural killer cell) 활성 검사

Natural killer cell activity of olive flounder Paralichthys olivaceus following intramuscular injection of toltrazuril derivative N-(4-(4-Fluorophenoxy)-3-methylphenyl) acetamide

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초록

This study assessed the impact of the toltrazuril derivative N-(4-(4-Fluorophenoxy)-3-methylphenyl) acetamide on natural cytotoxic cell (NCC) activity of olive flounder, Paralichthys olivaceus spleen. Five groups of fifteen olive flounder, comprising non-treatment and vehicle control groups, were ran-domly assigned. N-(4-(4-Fluorophenoxy)-3-methylphenyl) acetamide was injected intramuscularly at doses of 120, 150 and 200 mg/kg body weight; a total of ten injections were given over the course of 30 days. The NK activity of flounder splenic cells was evaluated against YAC-1, mouse lymphoma cells or HINAE cells with a choice of co-cultivation times of 4 or 18 hrs. In case of YAC-1 co-culture we observed a significant increase in cytotoxicity at a dose of 200 mg/kg, up to 3.06 times more than that of the control group. Only the trial with the 4 hrs co-culture produced a significant difference in the HINAE cell experiment; the experimental group at the 200 mg/kg dose exhibited the maximum cytotoxicity, demonstrating 2.3 times more cytotoxicity than the control group. Furthermore, the ex- pression level of IL-12b was markedly induced in the group with 200 mg/kg, which was 6.62 times greater than that of the control group. In terms of the altered NK cell activity, the repeated high doses of N-(4-(4-Fluorophenoxy)-3-methylphenyl) acetamide can cause changes in the normal performance of immune function.

Abstract

This study assessed the impact of the toltrazuril derivative N-(4-(4-Fluorophenoxy)-3-methylphenyl) acetamide on natural cytotoxic cell (NCC) activity of olive flounder, Paralichthys olivaceus spleen. Five groups of fifteen olive flounder, comprising non-treatment and vehicle control groups, were ran-domly assigned. N-(4-(4-Fluorophenoxy)-3-methylphenyl) acetamide was injected intramuscularly at doses of 120, 150 and 200 mg/kg body weight; a total of ten injections were given over the course of 30 days. The NK activity of flounder splenic cells was evaluated against YAC-1, mouse lymphoma cells or HINAE cells with a choice of co-cultivation times of 4 or 18 hrs. In case of YAC-1 co-culture we observed a significant increase in cytotoxicity at a dose of 200 mg/kg, up to 3.06 times more than that of the control group. Only the trial with the 4 hrs co-culture produced a significant difference in the HINAE cell experiment; the experimental group at the 200 mg/kg dose exhibited the maximum cytotoxicity, demonstrating 2.3 times more cytotoxicity than the control group. Furthermore, the ex- pression level of IL-12b was markedly induced in the group with 200 mg/kg, which was 6.62 times greater than that of the control group. In terms of the altered NK cell activity, the repeated high doses of N-(4-(4-Fluorophenoxy)-3-methylphenyl) acetamide can cause changes in the normal performance of immune function.

참고문헌 (70)

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관련 논문AI추천

넙치, Paralichthys olivaceus를 이용한 toltrazuril의 안전성 평가: 급성독성, 약물 대사, 사이토카인 유전자 발현 및 생리학적 지표 분석김명석, 김아란, 김이경, 도정완, 박상협, 조미영수산생명의학회 · 한국어병학회지2024 · 제37권 제2호레바미졸이 틸라피아(Oreochromis niloticus)의 자연살해세포 활성에 미치는 영향박관하, 윤종만, 최상훈한국수산과학회 · 한국수산과학회지2012 · 제45권 제6호톨트라주릴(Toltrazuril)을 이용한 제주도 넙치(Paralichthys olivaceus)의 점액포자충성 여윔증에 대한 치료법 연구강미래, 김성현, 김승민, 김예지, 전려진, 정준범, 한소리수산생명의학회 · 한국어병학회지2020 · 제33권 제1호LC-MS/MS를 이용한 넙치(Paralichthys olivaceus)시료의 톨트라주릴 및 톨트라주릴 설폰 분석김나영, 김명석, 김아현, 손광태, 윤민철, 이가정, 정승희, 조미라, 홍도희한국수산과학회 · 한국수산과학회지2019 · 제52권 제5호Amprolium hydrochloride 투여에 따른 넙치(Paralichthys olivaceus)의 독성 영향김이경, 도정완, 박상협, 최영광, 최혜승수산생명의학회 · 한국어병학회지2023 · 제36권 제1호Amprolium hydrochloride가 넙치 Paralichthys olivaceus의 약물대사 유전자 발현에 미치는 영향김이경, 김창환, 도정완, 박상협, 최혜승수산생명의학회 · 한국어병학회지2023 · 제36권 제2호

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