LC-MS/MS를 이용한 넙치(Paralichthys olivaceus)시료의 톨트라주릴 및 톨트라주릴 설폰 분석
Determinations of Toltrazuril and Toltrazuril Sulfone Levels in Olive Flounder Paralichthys olivaceus Samples Using Liquid Chromatography- Electrospray Ionization Tandem Mass Spectrometry
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Several studies investigating the prevention and treatment of external parasites in farmed olive flounder Paralichthys olivaceus have found that the anticoccidial agent toltrazuril sulfone is an effective antiparasitic. Prior to undertaking a full-scale study, we developed analytical methods to detect the levels of toltrazuril and toltrazuril sulfone in farmed flounder samples using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS). This analysis showed that LC-MS/MS changed the mobile phase and collision energy of toltrazuril and toltrazuril sulfone. This was validated using established conditions. Sample pre-treatment for this process involved extraction with dichloromethane and purification by liquid–liquid extraction in formic acid, acetonitrile, and h-hexane, followed by determination of all compounds by LC-MS/MS. Separation was achieved within 10 min by gradient elution using a Capcell Pak C18 (3.0 μm, 100×2.0 mm) analytical column (Shiseido UG 120V) with a mixture of 0.1% (v/v) formic acid and acetonitrile. Multiple reaction monitoring was used for selective detection of toltrazuril and toltrazuril sulfone. This method yields satisfactory results for linearity, precision, and limits of quantification. Therefore, the method established in our study will serve as a basis for further research on parasite control by toltrazuril and toltrazuril sulfone.
Abstract
Several studies investigating the prevention and treatment of external parasites in farmed olive flounder Paralichthys olivaceus have found that the anticoccidial agent toltrazuril sulfone is an effective antiparasitic. Prior to undertaking a full-scale study, we developed analytical methods to detect the levels of toltrazuril and toltrazuril sulfone in farmed flounder samples using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS). This analysis showed that LC-MS/MS changed the mobile phase and collision energy of toltrazuril and toltrazuril sulfone. This was validated using established conditions. Sample pre-treatment for this process involved extraction with dichloromethane and purification by liquid–liquid extraction in formic acid, acetonitrile, and h-hexane, followed by determination of all compounds by LC-MS/MS. Separation was achieved within 10 min by gradient elution using a Capcell Pak C18 (3.0 μm, 100×2.0 mm) analytical column (Shiseido UG 120V) with a mixture of 0.1% (v/v) formic acid and acetonitrile. Multiple reaction monitoring was used for selective detection of toltrazuril and toltrazuril sulfone. This method yields satisfactory results for linearity, precision, and limits of quantification. Therefore, the method established in our study will serve as a basis for further research on parasite control by toltrazuril and toltrazuril sulfone.
참고문헌 (14)
- Ai L, "Determination of diclazuril, toltrazuril and its two metabolites in poultry tissues and eggs by gel permeation chromatography-liquid chromatography-tandem mass spectrometry", J Chromato B, 879, pp.1757~1763 (2011)[DOI]
- Buiarelli F, "Liquid chromatography tandem mass spectrometry analysis of synthetic coccidiostat in eggs", Separations, 4, pp.1~12 (2017)[DOI]
- Causon R, "Validation of chromatographic methods in biomedical analysis viewpoint and discussion", J Chromatogr B, 689, pp.175~180 (1997)[DOI]
- Clarke L, "A review of coccidiostats and the analysis of their residues in meat and other food", Meat Sci, 97, pp.358~374 (2014)[DOI]
- Committee for veterinary medicinal products, toltrazuril, Summary report (1), "The European Agency for the Evaluation of Medicinal Products Veterinary Medicines Evaluation Unit", EMEA, ~, pp.EMEA (1998), MRL[DOI]
- Frank RP, "Effect of a single dose of ponazuril on neural infection and clinical disease in Sarcocystis neurona-challenged interferon-gamma knock-out mice", Vet Parasitol, 114, pp.23~130 (2003)[DOI]
- Furr M, "Efficacy of ponazuril 15% oral paste as a treatment for equine protozoal Myeloencephalitis", Vet Therapeu, 2, pp.215~222
- Myoung-Seok Kim, "Plasma disposition of toltrazuril and its metabolites, toltrazuril sulfoxide and toltrazuril sulfone, in rabbits after oral administration", Veterinary Parasitology, 169(1-2), pp.51~56 (2010), Elsevier BV[DOI]
- "Korea food code. Chart 8. General methods 8.3.3 Nicarbazine, decoquinate, dicyclanil, diaveridine, diclazuril, robenidine, roxarsone, amprolium, ethopabate, imidocarb, zoalene, clopidol, halofuginone, toltrazuril" (2019)[URL]
- Moloney M, "Determination of 20 coccidiostats in egg and avian muscle tissue using ultra high performance liquid chromatography-tandem mass spectrometry", J Chromato A, 1253, pp.94~104 (2012)[DOI]
- Li H, "Analysis of veterinary drug residues in shrimp : A multi-class method by liquid chromatography-quadrupole ion trap mass spectrometry", J Chromato B, 836, pp.22~38 (2006)[DOI]
- Lindsay DS, "Determination of the activity of ponazuril against Sarcocystis neuron in cell cultures", Vet Parasitol, 92, pp.165~169 (2000)[DOI]
- Maes D, "Effects of toltrazuril on the growth of piglets in herds without clinical isosporosis", Vet J, 173, pp.197~199 (2007)[DOI]
- Olsen J, "Development of an analytical methodology for the determination of the antiparasitic drug toltrazuril and its two metabolites in surface water, soil and animal manure", Analytica Chimica Acta, 755, pp.69~76 (2012)[DOI]