넙치, Paralichthys olivaceus를 이용한 toltrazuril의 안전성 평가: 급성독성, 약물 대사, 사이토카인 유전자 발현 및 생리학적 지표 분석
Effects of toltrazuril on olive flounder (Paralichthys olivaceus): Acute toxicity, Drug metabolism, Cytokine gene expression and Biochemical parameters
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The aim of this study was to examine the effect of toltrazuril on olive flounder Paralichthys olivaceus. Sixty flounder (average weight 107.75 ± 8.48 g; average length 21.01 ± 1.13 cm) was ran-domly allocated into six groups, namely, 5, 10, 20, 50, 100 and 200 mg/kg. As measure of acute toxicity the LD50 value of 108.904 mg/kg was obtained. Based on the results of the acute toxicity test, a range-finding test was conducted by intramuscularly injecting toltrazuril once daily for three consecutive days. The doses were as follows: [1] 20, 40, 60, 80 mg/kg (low-dose group) and [2] 100, 120, 150 mg/kg (high-dose group). Subsequently, gene expression analysis and biochemical test were performed, leading to the determination of the no observed adverse effect level (NOAEL) as 80 mg/kg. In conclusion, toltrazuril has a high toxicity in olive flounder, and further studies are required to more evaluate the toxicological mechanisms of toltrazuril. This study will provide essential data for assessing the safety and ecological risks of toltrazuril.
Abstract
The aim of this study was to examine the effect of toltrazuril on olive flounder Paralichthys olivaceus. Sixty flounder (average weight 107.75 ± 8.48 g; average length 21.01 ± 1.13 cm) was ran-domly allocated into six groups, namely, 5, 10, 20, 50, 100 and 200 mg/kg. As measure of acute toxicity the LD50 value of 108.904 mg/kg was obtained. Based on the results of the acute toxicity test, a range-finding test was conducted by intramuscularly injecting toltrazuril once daily for three consecutive days. The doses were as follows: [1] 20, 40, 60, 80 mg/kg (low-dose group) and [2] 100, 120, 150 mg/kg (high-dose group). Subsequently, gene expression analysis and biochemical test were performed, leading to the determination of the no observed adverse effect level (NOAEL) as 80 mg/kg. In conclusion, toltrazuril has a high toxicity in olive flounder, and further studies are required to more evaluate the toxicological mechanisms of toltrazuril. This study will provide essential data for assessing the safety and ecological risks of toltrazuril.
참고문헌 (81)
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